Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	161	75.5	2581324	99.3%	2563254	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,813,274	T→C	L220P (CTC→CCC)	emrB →	multidrug efflux pump membrane subunit EmrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,813,274	0	T	C	88.9%	178.4 / 10.8	63	L220P (CTC→CCC)	emrB	multidrug efflux pump membrane subunit EmrB
Reads supporting (aligned to +/- strand): ref base T (3/4); new base C (28/28); total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

GTGCCGATTGGCGTGGCGGTGGTGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTTGTT  >  NC_000913/2813138‑2813409

gTGCCGATTGGCGTGGCGGTGGTGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGa                                                                                                                                      <  1:874391/140‑1 (MQ=255)

gTGCCGATTGGCGTGGCGGTGGTGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGa                                                                                                                                      <  1:513673/140‑1 (MQ=255)

 tGCCGATTGGCGTGGCGGTGGGGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGAc                                                                                                                                     >  1:1144214/1‑140 (MQ=255)

   ccGATTGGCGTGGCGGTGGTGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACcg                                                                                                                                   >  2:389892/1‑140 (MQ=255)

           cgtggcgGTGGTGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAag                                                                                                                           >  2:682998/1‑140 (MQ=255)

           cgtggcgGTGGTGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAag                                                                                                                           <  2:115407/140‑1 (MQ=255)

                     gtgtTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGa                                                                                                                    >  2:675268/1‑137 (MQ=255)

                     gtgtTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGa                                                                                                                    <  1:675268/137‑1 (MQ=255)

                     gggtTGATGACACTGCAAACTCTGCGCGGGCGTGGAACCCGCACCGAACGGCGGCGGGTTGATGCCGTGGGGGTGGCACCGGTGGGTATTGGGATCGGCAGCCTGCAGAATATGCCCCACCGCGGGAAAGAGCTGGACTg                                                                                                                 >  2:745765/3‑140 (MQ=255)

                               cacTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGa                                                                                                                    <  2:147755/127‑1 (MQ=255)

                               cacTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGa                                                                                                                    >  1:147755/1‑127 (MQ=255)

                                acTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATcac                                                                                                      >  2:943098/1‑140 (MQ=255)

                                  tGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAg                                                                                                    >  2:1059953/1‑140 (MQ=255)

                                    cAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGa                                                                                                  <  1:482266/140‑1 (MQ=255)

                                    cAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGa                                                                                                  >  2:132452/1‑140 (MQ=255)

                                    cAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGa                                                                                                  <  2:345131/140‑1 (MQ=255)

                                     aaaCTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGaa                                                                                                 >  1:1281958/1‑140 (MQ=255)

                                         tctGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTa                                                                                             >  2:403572/1‑140 (MQ=255)

                                          ctGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTat                                                                                            <  2:356373/140‑1 (MQ=255)

                                          ctGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTat                                                                                            <  1:424684/140‑1 (MQ=255)

                                          ctGCGCGGACGTGAAACCCGCACCGAACGGCAGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTat                                                                                            <  1:913070/140‑1 (MQ=255)

                                            gcgcGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTatca                                                                                          >  2:1105085/1‑140 (MQ=255)

                                             cgcgGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTatcat                                                                                         <  1:1273404/140‑1 (MQ=255)

                                                ggACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCt                                                                                      <  1:1069796/140‑1 (MQ=255)

                                                  aCGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTa                                                                                    >  1:448599/1‑140 (MQ=255)

                                                  aCGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTa                                                                                    >  1:1108334/1‑140 (MQ=255)

                                                    gTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTAcc                                                                                  <  1:943098/140‑1 (MQ=255)

                                                       aaaCCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCgtg                                                                               <  1:1271928/140‑1 (MQ=255)

                                                            cGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGc                                                                          >  2:1016933/1‑140 (MQ=255)

                                                            cGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGc                                                                          >  2:556805/1‑140 (MQ=255)

                                                            cGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGc                                                                          >  1:1103571/1‑140 (MQ=255)

                                                              cACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCgg                                                                        >  2:672792/1‑140 (MQ=255)

                                                                  gAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGATTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCggtggt                                                                    <  1:682998/140‑1 (MQ=255)

                                                                   aaCGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGgtggtg                                                                   >  1:881841/1‑140 (MQ=255)

                                                                     cggcggcggATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGc                                                                 <  2:380304/140‑1 (MQ=255)

                                                                        cggcggATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTAt                                                              <  1:132452/140‑1 (MQ=255)

                                                                        cggcggATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTAt                                                              <  2:881841/140‑1 (MQ=255)

                                                                        cggcggATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTAt                                                              <  2:879640/140‑1 (MQ=255)

                                                                                tGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTcc                                                      >  1:277554/1‑140 (MQ=255)

                                                                                   tGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGa                                                   <  2:277554/140‑1 (MQ=255)

                                                                                   tGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGa                                                   <  2:1281958/140‑1 (MQ=255)

                                                                                       gTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGt                                               >  1:295085/1‑140 (MQ=255)

                                                                                        tGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTc                                              >  2:619478/1‑140 (MQ=255)

                                                                                         ggggCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCt                                             <  1:389892/140‑1 (MQ=255)

                                                                                             cTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGa                                         >  1:400702/1‑140 (MQ=255)

                                                                                              tGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAg                                        <  1:672792/140‑1 (MQ=255)

                                                                                                   ctgctgGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGAc                                   <  2:400702/140‑1 (MQ=255)

                                                                                                      ctgGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACcga                                >  2:716006/1‑140 (MQ=255)

                                                                                                      ctgGTTATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACcga                                >  2:1110935/1‑140 (MQ=255)

                                                                                                          ttATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGAt                            <  1:1059953/140‑1 (MQ=255)

                                                                                                          ttATTGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGAt                            >  2:47103/1‑140 (MQ=255)

                                                                                                              tGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGAt                            <  1:246267/136‑1 (MQ=255)

                                                                                                              tGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGAt                            >  2:246267/1‑136 (MQ=255)

                                                                                                              tGGTATCGGCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAAcc                        <  2:1103571/140‑1 (MQ=255)

                                                                                                                     ggCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGt                 <  2:295085/140‑1 (MQ=255)

                                                                                                                     ggCAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGt                 >  2:921242/1‑140 (MQ=255)

                                                                                                                     ggCAGCCTCCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGt                 >  2:905533/1‑140 (MQ=255)

                                                                                                                       cAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACcgac                               >  1:1201390/1‑124 (MQ=255)

                                                                                                                       cAGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACcgac                               <  2:1201390/124‑1 (MQ=255)

                                                                                                                        aGCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGa              >  2:29986/1‑140 (MQ=255)

                                                                                                                         gCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGAt             <  1:1173293/140‑1 (MQ=255)

                                                                                                                         gCCTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGAt             <  1:1016933/140‑1 (MQ=255)

                                                                                                                           cTGCAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCt           >  2:1110597/1‑140 (MQ=255)

                                                                                                                              cAGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTc        <  1:921242/140‑1 (MQ=255)

                                                                                                                               aGATTATGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCg       >  1:678996/1‑140 (MQ=255)

                                                                                                                                    aTGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCgttgtt  >  1:269091/1‑140 (MQ=255)

                                                                                                                                    aTGCCCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCgttgtt  >  1:948835/1‑140 (MQ=255)

GTGCCGATTGGCGTGGCGGTGGTGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTTGTT  >  NC_000913/2813138‑2813409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A