Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	99,352	T→C	L317S (TTA→TCA)	ftsW →	putative lipid II flippase FtsW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	99,352	0	T	C	100.0%	125.5 / NA	36	L317S (TTA→TCA)	ftsW	putative lipid II flippase FtsW
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/15); total (21/15)

AAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAAC  >  NC_000913/99238‑99479

aaGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTcgc                                                                                                        <  1:210054/140‑1 (MQ=255)

   gTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTCTcgcgcg                                                                                                     <  2:120121/140‑1 (MQ=255)

           aaCTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGAt                                                                                             <  1:356148/140‑1 (MQ=255)

             cTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGt                                                                                                 <  2:980059/134‑1 (MQ=255)

             cTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGt                                                                                                 >  1:980059/1‑134 (MQ=255)

             cTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTg                                                                                           >  2:41027/1‑140 (MQ=255)

             cTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTg                                                                                           >  1:735133/1‑140 (MQ=255)

                       aaaCTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGc                                                                                 >  2:930998/1‑140 (MQ=255)

                             gAGTATCTGCCGGAAGCGCACACTGAC‑TTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGaa                                                                          <  2:581168/140‑1 (MQ=255)

                               gTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGaaa                                                                         >  1:391800/1‑140 (MQ=255)

                                  tCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCGTTAGAAATTg                                                                      >  1:699411/1‑140 (MQ=255)

                                  tCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTg                                                                      >  2:986546/1‑140 (MQ=255)

                                                 cacTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCg                                                       <  1:89516/140‑1 (MQ=255)

                                                 cacTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCg                                                       <  1:930998/140‑1 (MQ=255)

                                                      aCTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGtttt                                                  >  1:118505/1‑140 (MQ=255)

                                                      aCTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGtttt                                                  >  2:661985/1‑140 (MQ=255)

                                                        tttATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTtct                                                <  2:699411/140‑1 (MQ=255)

                                                        tttATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTtct                                                <  1:661985/140‑1 (MQ=255)

                                                          tATTTTCGCCATTATCGGCGAAGAACCGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCg                                              >  1:842842/1‑140 (MQ=255)

                                                                cGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGtt                                        >  2:689182/1‑140 (MQ=255)

                                                                cGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGtt                                        >  2:996303/1‑140 (MQ=255)

                                                                   cATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTa                                     <  1:654633/140‑1 (MQ=255)

                                                                    aTTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTAt                                    <  1:986546/140‑1 (MQ=255)

                                                                               agaaCTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTAt                                    >  2:182463/1‑129 (MQ=255)

                                                                               agaaCTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTAt                                    <  1:182463/129‑1 (MQ=255)

                                                                                gaaCTGGGGTATGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGtt                        <  2:391800/140‑1 (MQ=255)

                                                                                           tGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAgg             >  1:677131/1‑140 (MQ=255)

                                                                                           tGTCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAgg             >  1:148770/1‑140 (MQ=255)

                                                                                             tCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGgcg           <  2:500653/140‑1 (MQ=255)

                                                                                             tCGGTGTGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGgcg           >  2:558332/1‑140 (MQ=255)

                                                                                                gtgtGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTg        >  1:82702/1‑140 (MQ=255)

                                                                                                gtgtGGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTg        <  2:82702/140‑1 (MQ=255)

                                                                                                 tgtgGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTgg       >  2:512438/1‑140 (MQ=255)

                                                                                                 tgtgGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTgg       >  1:565646/1‑140 (MQ=255)

                                                                                                 tgtgGTGCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTgg       >  1:538769/1‑140 (MQ=255)

                                                                                                      tgCTGGCACTTTCAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAAc  >  2:911078/1‑140 (MQ=255)

AAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAAC  >  NC_000913/99238‑99479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A