Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,046,673	T→C	N790S (AAC→AGC)	gcvP ←	glycine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,046,673	0	T	C	100.0%	193.0 / NA	55	N790S (AAC→AGC)	gcvP	glycine decarboxylase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (36/19); total (36/19)

GCTTGGCAATATCCAGCTCGCTGATGCCGGTTTCTTCTTTCAGCGGGCGAATATCGAGAATACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGTTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACGGTGCCGCAGAAACCGCGCCCTGACGG  >  NC_000913/3046536‑3046801

gcTTGGCAATATCCAGCTCGCTGATGCCGGTTTCTTCTTTCAGCGGGCGAATATCGAGAATACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTg                                                                                                                                <  1:916959/140‑1 (MQ=255)

     gCAATATCCAGCTCGCTGATGCCGGTTTCTTCTTTCAGCGGGCGAATATCGAGAATACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGtt                                                                                                                           <  1:888389/140‑1 (MQ=255)

        atatCCAGCTCGCTGATGCCGGTTTCTTCTTTCAGCGGGCGAATATCGAGAATACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAg                                                                                                                        >  2:714074/1‑140 (MQ=255)

                cTCGCTGATGCCGGTTTCTTCTTTCAGCGGGCGAATATCGAGAATACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGAtt                                                                                                                    >  2:609420/1‑136 (MQ=255)

                cTCGCTGATGCCGGTTTCTTCTTTCAGCGGGCGAATATCGAGAATACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGAtt                                                                                                                    <  1:609420/136‑1 (MQ=255)

                cTCGCTGATGCCGGTTTCTTCTTTCAGCGGGCGAATATCGAGAATACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCa                                                                                                                >  2:91101/1‑140 (MQ=255)

                cTCGCTGATGCCGGTTTCTTCTTTCAGCGGGCGAATATCGAGAATACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCa                                                                                                                >  2:437745/1‑140 (MQ=255)

                cTCGCTGATGCCGGTTTCTTCTTTCAGCGGGCGAATATCGAGAATACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCa                                                                                                                >  1:644619/1‑140 (MQ=255)

                     tGATGCCGGTTTCTTCTTTCAGCGGGCGAATATCGAGAATACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTgg                                                                                                           >  2:969293/1‑140 (MQ=255)

                     tGATGCCGGTTTCTTCTTTCAGCGGGCGAATATCGAGAATACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTgg                                                                                                           >  2:862076/1‑140 (MQ=255)

                                           cGGGCGAATATCGAGAATACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTg                                                                                     >  1:489571/1‑140 (MQ=255)

                                            gggCGAATATCGAGAATACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCtttt                                                                                                  <  2:724786/126‑1 (MQ=255)

                                            gggCGAATATCGAGAATACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCtttt                                                                                                  >  1:724786/1‑126 (MQ=255)

                                             ggCGAATATCGAGAATACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTgcg                                                                                   >  1:347647/1‑140 (MQ=255)

                                             ggCGAATATCGAGAATACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTgcg                                                                                   <  1:969293/140‑1 (MQ=255)

                                               cGAATATCGAGAATACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGcc                                                                                 >  2:584255/1‑140 (MQ=255)

                                                        agaATACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCg                                                                        >  1:619907/1‑140 (MQ=255)

                                                        agaATACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCg                                                                        <  2:781701/140‑1 (MQ=255)

                                                            tACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGAt                                                                     <  1:67318/139‑1 (MQ=255)

                                                            tACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGAt                                                                     >  2:67318/1‑139 (MQ=255)

                                                            tACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATg                                                                    >  1:846843/1‑140 (MQ=255)

                                                               aTTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCca                                                                               >  2:946946/1‑126 (MQ=255)

                                                               aTTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCca                                                                               <  1:946946/126‑1 (MQ=255)

                                                               aTTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTAc                                                                 >  1:815960/1‑140 (MQ=255)

                                                                ttCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGCAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACa                                                                >  2:906506/1‑140 (MQ=255)

                                                                    tGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATg                                                                          <  2:69680/126‑1 (MQ=255)

                                                                    tGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATg                                                                          >  1:69680/1‑126 (MQ=255)

                                                                    tGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCa                                                            >  1:236680/1‑140 (MQ=255)

                                                                    tGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCa                                                            >  2:367460/1‑140 (MQ=255)

                                                                    tGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGCTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCg                                                                        >  1:296578/1‑128 (MQ=255)

                                                                    tGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGCTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCg                                                                        <  2:296578/128‑1 (MQ=255)

                                                                             cgACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTgg                                                   <  1:91101/140‑1 (MQ=255)

                                                                                   tCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCATTATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGAt                                             >  2:101114/1‑140 (MQ=255)

                                                                                        aCCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAgg                                        <  1:906506/140‑1 (MQ=255)

                                                                                        aCCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAgg                                        <  1:367460/140‑1 (MQ=255)

                                                                                        aCCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAgg                                        <  1:862076/140‑1 (MQ=255)

                                                                                                    cACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACg                            >  1:718915/1‑140 (MQ=255)

                                                                                                    cACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACg                            >  1:218599/1‑140 (MQ=255)

                                                                                                    cACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACg                            >  1:106784/1‑140 (MQ=255)

                                                                                                      ccGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACGGt                          >  2:509598/1‑140 (MQ=255)

                                                                                                       cGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACGGTg                         >  1:869496/1‑140 (MQ=255)

                                                                                                       cGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACGGTg                         >  2:701594/1‑140 (MQ=255)

                                                                                                       cGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACGGTg                         >  1:57519/1‑140 (MQ=255)

                                                                                                       cGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACGGTg                         >  2:89340/1‑140 (MQ=255)

                                                                                                       cGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACGGTg                         >  1:83491/1‑140 (MQ=255)

                                                                                                         ggAAGGCATCCTGCAGGCGGCTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACGGTGcc                       >  2:714453/1‑140 (MQ=255)

                                                                                                                        ggcggcTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACGGTGCCGCAGAAACCGCGccc        >  2:752168/1‑140 (MQ=255)

                                                                                                                         gcggcTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACGGTGCCGCAGAAACCGCGCCCt       >  1:742370/1‑140 (MQ=255)

                                                                                                                         gcggcTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACGGTGCCGCAGAAACCGCGCCCt       >  1:976713/1‑140 (MQ=255)

                                                                                                                           ggcTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACGGTGCCGCAGAAACCGCGCCCTGa     <  2:869496/140‑1 (MQ=255)

                                                                                                                           ggcTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACGGTGCCGCAGAAACCGCGCCCTGa     <  1:437745/140‑1 (MQ=255)

                                                                                                                             cTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACGGTGCCGCAGAAACCGCGCCCTg      >  2:129056/1‑137 (MQ=255)

                                                                                                                             cTGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACGGTGCCGCAGAAACCGCGCCCTg      <  1:129056/137‑1 (MQ=255)

                                                                                                                              tGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACGGTGCCGCAGAAACCGCGCCCTGACgg  <  2:846843/140‑1 (MQ=255)

                                                                                                                              tGGCAATATAGCTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACGGTGCCGCAGAAACCGCGCCCTGACgg  <  2:619907/140‑1 (MQ=255)

GCTTGGCAATATCCAGCTCGCTGATGCCGGTTTCTTCTTTCAGCGGGCGAATATCGAGAATACATTCGTGCGCCACGCGACCGTCGCGACCGGTATACAGCACCGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGTTGGCGTTGAGGATTGCCACCTGGCTTGCTTTTTTCAGCCCTTCTGCGCCCATCATGCGGATGTACATCCAGCTGATTGGCAGGATAGAGGCGCTACCGAACGGTGCCGCAGAAACCGCGCCCTGACGG  >  NC_000913/3046536‑3046801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A