Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 155 60.3 2039550 99.3% 2025273 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,141,148 C→T E47K (GAG→AAG)  hybD ← putative hydrogenase 2 maturation protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,141,1480CT100.0% 183.9 / NA 55E47K (GAG→AAG) hybDputative hydrogenase 2 maturation protease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/27);  total (28/27)

GGAGAGATTTTGTTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGC  >  NC_000913/3141012‑3141284
                                                                                                                                        |                                                                                                                                        
ggAGAGATTTTGTTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCACCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCAt                                                                                                                                       <  1:755558/140‑1 (MQ=255)
 gagagaTTTTGTTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCAtt                                                                                                                                      >  1:299904/1‑140 (MQ=255)
              ggTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTgccgc                                                                                                                         >  1:169703/1‑140 (MQ=255)
              ggTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTgccgc                                                                                                                         >  1:983742/1‑140 (MQ=255)
                tAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCa                                                                                                                       >  2:794035/1‑140 (MQ=255)
                     aacGCCGGACCTTCTTCATCCCGCAGGATCCTCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAg                                                                                                                  <  2:624864/140‑1 (MQ=255)
                       cGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGa                                                                                                                >  1:379333/1‑140 (MQ=255)
                         ccGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGAtc                                                                                                              >  1:864542/1‑140 (MQ=255)
                         ccGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGAt                                                                                                               >  1:121358/1‑139 (MQ=255)
                         ccGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGAt                                                                                                               <  2:121358/139‑1 (MQ=255)
                          cGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGAtct                                                                                                             <  2:766972/140‑1 (MQ=255)
                             aaCTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCaa                                                                                                          <  2:571485/140‑1 (MQ=255)
                                    tcATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATc                                                                                                   <  2:130931/140‑1 (MQ=255)
                                       tCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCgg                                                                                                >  1:248895/1‑140 (MQ=255)
                                       tCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCgg                                                                                                <  2:253801/140‑1 (MQ=255)
                                       ccccGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCgg                                                                                                <  2:44889/139‑1 (MQ=255)
                                        cccGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGc                                                                                               <  1:447682/140‑1 (MQ=255)
                                            cAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGaa                                                                                           >  1:943588/1‑140 (MQ=255)
                                             aGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAAt                                                                                          >  1:247583/1‑140 (MQ=255)
                                                         gTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGctc                                                                              >  1:865698/1‑140 (MQ=255)
                                                         gTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGctc                                                                              >  2:320188/1‑140 (MQ=255)
                                                          ttCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTAt                                                                                      <  1:894544/131‑1 (MQ=255)
                                                          ttCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTAt                                                                                      >  2:894544/1‑131 (MQ=255)
                                                            cccGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTaa                                                                           >  1:223316/1‑140 (MQ=255)
                                                            cccGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTaa                                                                           <  2:864542/140‑1 (MQ=255)
                                                               ggCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGc                                                                        >  1:311730/1‑140 (MQ=255)
                                                                gcgcGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCt                                                                       >  2:995055/1‑140 (MQ=255)
                                                                  gcgTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTc                                                                     >  2:577286/1‑140 (MQ=255)
                                                                   cgTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCg                                                                    <  1:995055/140‑1 (MQ=255)
                                                                    gTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGa                                                                   >  2:852166/1‑140 (MQ=255)
                                                                     ttCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGAc                                                                  <  2:560957/140‑1 (MQ=255)
                                                                      tCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACa                                                                 <  1:582289/140‑1 (MQ=255)
                                                                           ttCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCg                                                            >  2:493478/1‑140 (MQ=255)
                                                                                  cAATGCCATCCGCAATAACCAAATGATCGGGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCg                                                     <  2:379333/140‑1 (MQ=255)
                                                                                    aTGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGAt                                                   <  2:299904/140‑1 (MQ=255)
                                                                                    aTGGCATCCGCAATAAGAAAATGATCGCGATTTGCCATGTCGCCAAGCATCTTCATCCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGAt                                                   <  2:248895/140‑1 (MQ=255)
                                                                                          tCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTc                                             >  1:158793/1‑140 (MQ=255)
                                                                                             gCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATc                                          >  1:200678/1‑140 (MQ=255)
                                                                                                      aaaTGATCGCGATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCaa                                 >  2:718874/1‑140 (MQ=255)
                                                                                                             tgcgATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCa                                  <  2:279899/131‑1 (MQ=255)
                                                                                                             tgcgATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCa                                  >  1:279899/2‑132 (MQ=255)
                                                                                                             cgcgATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATCATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTg                          >  2:209889/1‑140 (MQ=255)
                                                                                                             cgcgATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTg                          >  2:728860/1‑140 (MQ=255)
                                                                                                              gcgATTTGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGc                         >  2:47913/1‑140 (MQ=255)
                                                                                                                 ttttGCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGa                      <  2:26222/139‑1 (MQ=255)
                                                                                                                     gCCATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGAcccc                  <  2:943588/140‑1 (MQ=255)
                                                                                                                      ccATGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCt                 <  2:247583/140‑1 (MQ=255)
                                                                                                                        aTGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTaa               <  1:718874/140‑1 (MQ=255)
                                                                                                                        aTGTCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTaa               <  1:728860/140‑1 (MQ=255)
                                                                                                                           tCGCCAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGAc            >  1:922141/1‑140 (MQ=255)
                                                                                                                               cAAGCAGCTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTaaa        <  2:200678/140‑1 (MQ=255)
                                                                                                                                    agcTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACg   >  1:299154/1‑140 (MQ=255)
                                                                                                                                    agcTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACg   <  1:320188/140‑1 (MQ=255)
                                                                                                                                    agcTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACg   <  2:597614/140‑1 (MQ=255)
                                                                                                                                    agcTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACg   <  1:852166/140‑1 (MQ=255)
                                                                                                                                     gcTTCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGc  <  1:577286/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GGAGAGATTTTGTTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGC  >  NC_000913/3141012‑3141284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A