Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I1 R1
|
155 |
60.3 |
2039550 |
99.3% |
2025273 |
138.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,350,184 |
(C)7→9 |
coding (276/654 nt) |
elbB ← |
low activity glyoxalase ElbB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,350,177 | 1 | . | C | 100.0%
| 164.3
/ NA
| 48 | F95V (TTT→GTT) | elbB | low activity glyoxalase ElbB |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (26/22); total (26/22) |
| * | NC_000913 | 3,350,177 | 2 | . | C | 97.9%
| 161.9
/ ‑3.3
| 48 | coding (283/654 nt) | elbB | low activity glyoxalase ElbB |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (25/22); total (26/22) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 |
GGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAA‑‑‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAA > NC_000913/3350049‑3350306
||
ggCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAA‑‑‑ccccccc < 1:715845/136‑1 (MQ=255)
ggCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAA‑‑‑ccccccc > 2:715845/1‑136 (MQ=255)
aTAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAAc > 2:427331/1‑140 (MQ=255)
aTAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑‑CCCCCCCCGCACAATCAAcc > 2:373038/1‑139 (MQ=255)
aaaCCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAAc > 2:823342/1‑137 (MQ=255)
aaaCCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAAc < 1:823342/137‑1 (MQ=255)
aCCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAAc < 2:616600/135‑1 (MQ=255)
aCCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAAc > 1:616600/1‑135 (MQ=255)
aCCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATc < 1:519006/140‑1 (MQ=255)
aCCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATc < 1:713880/140‑1 (MQ=255)
aCCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATc > 2:702962/1‑140 (MQ=255)
aaGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAg > 1:395489/1‑140 (MQ=255)
aaGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAg > 1:872452/1‑140 (MQ=255)
aGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGt > 1:970915/1‑140 (MQ=255)
cGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTc < 2:539793/140‑1 (MQ=255)
gTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAg < 1:43142/140‑1 (MQ=255)
cGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCAt < 1:442866/140‑1 (MQ=255)
cGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCAt < 2:730854/140‑1 (MQ=255)
cGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACCGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCAt < 1:241044/140‑1 (MQ=255)
gCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACTGGCAACGGTGCATTCGCTACCAAGACTGGCAAAAGTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCg < 2:531008/140‑1 (MQ=255)
ccTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCgg > 2:131364/1‑140 (MQ=255)
tGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGcc < 2:161662/140‑1 (MQ=255)
aTGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCAt > 2:348394/1‑133 (MQ=255)
aTGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCAt < 1:348394/133‑1 (MQ=255)
aTGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTg > 2:515195/1‑140 (MQ=255)
cTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCagag > 2:583436/1‑140 (MQ=255)
tgtgCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGa > 2:210094/1‑140 (MQ=255)
gtgCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGAc > 2:289435/1‑140 (MQ=255)
tgCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACg > 1:819058/1‑140 (MQ=255)
aGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGAtt > 1:938161/1‑140 (MQ=255)
ccTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCAc < 1:427331/140‑1 (MQ=255)
cTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCAcc < 1:289435/140‑1 (MQ=255)
aCGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTAt > 2:260324/1‑140 (MQ=255)
tCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACCCCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCg > 1:913775/1‑140 (MQ=255)
gTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCt < 2:970915/140‑1 (MQ=255)
gTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCt < 1:702962/140‑1 (MQ=255)
tGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGAGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCtt > 2:132692/1‑140 (MQ=255)
cATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCa > 2:307940/1‑140 (MQ=255)
aTTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCaa < 1:583436/140‑1 (MQ=255)
tCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATc < 1:210094/140‑1 (MQ=255)
tACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGca < 2:462878/140‑1 (MQ=255)
aCCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGcac > 2:130398/1‑140 (MQ=255)
aCCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGcac > 1:347283/1‑140 (MQ=255)
aCCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGCCCAGAGGACGGATTTCACCGCCCGTTATTCGTGCCGCTTCAATCAGcac > 2:189319/1‑140 (MQ=255)
ggCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGt < 2:303228/140‑1 (MQ=255)
gCAAAATTGCTTAAATTCTTCGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGtt > 2:287798/1‑140 (MQ=255)
tcttcGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGc < 1:988135/140‑1 (MQ=255)
tcttcGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGc < 1:131364/140‑1 (MQ=255)
ttcGCCGCGCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCa > 2:811740/1‑140 (MQ=255)
cgcgCCAAACC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTaa > 1:433528/1‑140 (MQ=255)
||
GGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAA‑‑‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAA > NC_000913/3350049‑3350306
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
