Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 155 60.3 2039550 99.3% 2025273 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,737,150 Δ1 bp coding (28/825 nt) bax ← putative glycoside hydrolase Bax

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,737,1470C.98.3% 241.7 / ‑1.8 58coding (31/825 nt)baxputative glycoside hydrolase Bax
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base . (27/30);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.78e-01

TTGCGAGAATACTCTTGTTTACTGCTTACCTGTTTATTACTAGCTTTAGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCA  >  NC_000913/3737008‑3737280
                                                                                                                                           |                                                                                                                                     
ttGCGAGAATACTCTTGTTTACTGCTTACCTGTTTATTACTAGCTTTAGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCGc                                                                                                                                       <  2:571558/140‑1 (MQ=255)
ttGCGAGAATACTCTTGTTTACTGCTTACCTGTTTATTACTAGCTTTAGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCGc                                                                                                                                       >  2:673210/1‑140 (MQ=255)
 tGCGAGAATACTCTTGTTTACTGCTTACCTGTTTATTACTAGCTTTAGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCGcc                                                                                                                                      <  2:128481/140‑1 (MQ=255)
 tGCGAGAATACTCTTGTTTACTGCTTACCTGTTTATTACTAGCTTTAGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCGcc                                                                                                                                      >  1:424887/1‑140 (MQ=255)
   cGAGAATACTCTTGTTTACTGCTTACCTGTTTATTACTAGCTTTAGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCGccca                                                                                                                                    >  1:298474/1‑139 (MQ=255)
   cGAGAATACTCTTGTTTACTGCTTACCTGTTTATTACTAGCTTTAGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCGccca                                                                                                                                    >  2:408112/1‑139 (MQ=255)
      gaATACTCTTGTTTACTGCTTACCTGTTTATTACTAGCTTTAGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCGcccatat                                                                                                                                 <  1:673210/140‑5 (MQ=255)
          aCTCTTGTTTACTGCTTACCTGTTTATTACTAGCTTTAGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATAtcgtc                                                                                                                            >  2:326164/1‑140 (MQ=255)
          aCTCTTGTTTACTGCTTACCTGTTTATTACTAGCTTTAGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATAtcgtc                                                                                                                            >  2:905169/1‑140 (MQ=255)
            tctTGTTTACTGCTTACCTGTTTATTACTAGCTTTAGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATcgtcgt                                                                                                                          >  1:859128/1‑140 (MQ=255)
                             cTGTTTATTACTAGCTTTAGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAatcat                                                                                                         <  1:983007/140‑1 (MQ=255)
                             cTGTTTATTACTAGCTTTAGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAatcat                                                                                                         <  2:258230/140‑1 (MQ=255)
                                tttATTACTAGCTTTAGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAg                                                                                                      >  1:78243/1‑140 (MQ=255)
                                tttATTACTAGCTTTAGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAg                                                                                                      <  2:737335/140‑1 (MQ=255)
                                  tattaCTAGCTTTAGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGt                                                                                                    >  1:44726/1‑140 (MQ=255)
                                          gCTTTAGTTAAGTGGGACTTTTGACTCGCTGTTTTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGACAAAATCATCAGGTCTCCTGGt                                                                                            >  1:242037/1‑140 (MQ=255)
                                          gCTTTAGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGt                                                                                            <  2:859128/140‑1 (MQ=255)
                                          gCTTTAGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGt                                                                                            <  2:50018/140‑1 (MQ=255)
                                            tttAGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCg                                                                                          >  2:407314/1‑140 (MQ=255)
                                               aGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGAt                                                                                       <  2:298474/140‑1 (MQ=255)
                                               aGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGAt                                                                                       >  1:331335/1‑140 (MQ=255)
                                               aGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGAt                                                                                       >  2:109997/1‑140 (MQ=255)
                                                    aGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAAt                                                                                  <  1:41601/140‑1 (MQ=255)
                                                    aGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAAt                                                                                  >  1:586022/1‑140 (MQ=255)
                                                    aGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAAt                                                                                  <  2:879475/140‑1 (MQ=255)
                                                        ggACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAAt                                                                                  <  1:533342/136‑1 (MQ=255)
                                                        ggACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAAt                                                                                  >  2:533342/1‑136 (MQ=255)
                                                           cTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCa                                                                           <  2:331335/140‑1 (MQ=255)
                                                           cTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTAAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCa                                                                           <  2:242037/140‑1 (MQ=255)
                                                            ttttGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCaa                                                                          <  2:1015347/140‑1 (MQ=255)
                                                            ttttGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCaa                                                                          <  1:408112/140‑1 (MQ=255)
                                                                      cTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCa                                                                           <  1:876801/129‑1 (MQ=255)
                                                                      cTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCa                                                                           >  2:876801/1‑129 (MQ=255)
                                                                                 tgCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAAt                                                     >  1:601076/1‑140 (MQ=255)
                                                                                 tgCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAAt                                                     >  2:20730/1‑140 (MQ=255)
                                                                                 tgCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAAt                                                     >  1:44488/1‑140 (MQ=255)
                                                                                 tgCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAAt                                                     >  1:183606/1‑140 (MQ=255)
                                                                                 tgCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAAt                                                     >  2:320287/1‑140 (MQ=255)
                                                                                 tgCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACACAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAAt                                                     >  2:49413/1‑140 (MQ=255)
                                                                                          gCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGtt                                            <  2:601076/140‑1 (MQ=255)
                                                                                          gCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGtt                                            <  2:44726/140‑1 (MQ=255)
                                                                                                  cTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTg                                           <  2:129090/133‑1 (MQ=255)
                                                                                                  cTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTg                                           >  1:129090/1‑133 (MQ=255)
                                                                                                    cACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCaaa                                  >  2:152197/1‑140 (MQ=255)
                                                                                                    cACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCaaa                                  >  1:772879/1‑140 (MQ=255)
                                                                                                        cGAAAACACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCat                              <  2:395143/140‑1 (MQ=255)
                                                                                                          aaaaCACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCatat                            >  1:249216/1‑140 (MQ=255)
                                                                                                           aaaCACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCatat                            >  2:532108/1‑139 (MQ=255)
                                                                                                           aaaCACCAGAGTGAGTAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCatat                            <  1:532108/139‑1 (MQ=255)
                                                                                                                   gagtgagtAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCCCAAATCATATCAAAAATAg                   >  2:159716/1‑140 (MQ=255)
                                                                                                                        agtAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGATCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTc              <  2:424887/140‑1 (MQ=255)
                                                                                                                          tAACATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCCCAAATCATATCAAAAATAGCTGTCaa            <  1:159716/140‑1 (MQ=255)
                                                                                                                            aCATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATTATATCAAAAATAGCTGTCAaga          <  1:109997/140‑1 (MQ=255)
                                                                                                                             cATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAagag         <  2:183606/140‑1 (MQ=255)
                                                                                                                             cATAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAagag         <  2:595796/140‑1 (MQ=255)
                                                                                                                              aTAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAg                   <  1:536318/129‑1 (MQ=255)
                                                                                                                              aTAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAagag         >  2:552378/1‑139 (MQ=255)
                                                                                                                              aTAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAagag         <  1:552378/139‑1 (MQ=255)
                                                                                                                              aTAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATACTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAg                   >  2:536318/1‑129 (MQ=255)
                                                                                                                               tAAGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCa       <  2:234277/140‑1 (MQ=255)
                                                                                                                                aaGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCa       >  2:290518/1‑140 (MQ=255)
                                                                                                                                aaGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCAc      >  2:973660/1‑140 (MQ=255)
                                                                                                                                 aGAATCATCG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCAcc     <  1:152197/140‑1 (MQ=255)
                                                                                                                                    atcatcG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCa  <  1:20730/140‑1 (MQ=255)
                                                                                                                                    atcatcG‑CCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCa  <  1:49413/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                     
TTGCGAGAATACTCTTGTTTACTGCTTACCTGTTTATTACTAGCTTTAGTTAAGTGGGACTTTTGACTCGCTGTTGTTGTGTGCGTCTTCGCTAACACCTCACTCGAAAACACCAGAGTGAGTAACATAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCA  >  NC_000913/3737008‑3737280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A