Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,092,336	T→C	T11A (ACC→GCC)	frvB ←	putative PTS enzyme IIBC component FrvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,092,336	0	T	C	98.6%	243.6 / ‑4.7	72	T11A (ACC→GCC)	frvB	putative PTS enzyme IIBC component FrvB
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (36/35); total (36/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

CGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAATTCCTTGTGAATAATTTTGCGACA  >  NC_000913/4092204‑4092472

cGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATcgcg                                                                                                                                   >  1:269100/1‑140 (MQ=255)

  cTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGAc                                                                                                                                 <  2:711037/140‑1 (MQ=255)

   tGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACa                                                                                                                                <  1:650489/140‑1 (MQ=255)

   tGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACa                                                                                                                                <  1:30448/140‑1 (MQ=255)

     gATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAAt                                                                                                                              <  2:490331/140‑1 (MQ=255)

       tAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATAc                                                                                                                            <  1:476404/140‑1 (MQ=255)

               tttCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGa                                                                                                                                  <  2:31839/126‑1 (MQ=255)

               tttCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGa                                                                                                                                  <  2:374664/126‑1 (MQ=255)

               tttCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGa                                                                                                                                  >  1:31839/1‑126 (MQ=255)

               tttCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGa                                                                                                                                  >  1:374664/1‑126 (MQ=255)

               tttCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGAt                                                                                                                    >  2:623357/1‑140 (MQ=255)

                 tCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGa                                                                                                                  <  2:995370/140‑1 (MQ=255)

                      gCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCa                                                                                                             <  2:269100/140‑1 (MQ=255)

                                  ttGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGTCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCt                                                                                                 >  2:949354/1‑140 (MQ=255)

                                  ttGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGcc                                                                                                        <  2:125067/133‑1 (MQ=255)

                                  ttGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGcc                                                                                                        >  1:125067/1‑133 (MQ=255)

                                  ttGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCt                                                                                                 >  2:709328/1‑140 (MQ=255)

                                         tCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGc                                                                                          >  2:849592/1‑140 (MQ=255)

                                         tCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGCTTGCGATCGCGGCAATACGTAAGGATGACTCCATCCCCCCTTCCTTATTAGc                                                                                          >  1:732982/1‑140 (MQ=255)

                                          ccACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCt                                                                                         <  1:623357/140‑1 (MQ=255)

                                                ttATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGt                                                                                   >  1:982599/1‑140 (MQ=255)

                                                 tATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGtt                                                                                  <  2:150765/140‑1 (MQ=255)

                                                        tGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGGTTGATTTa                                                                           >  2:506018/1‑140 (MQ=255)

                                                          aCCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTg                                                                                        >  2:894635/1‑125 (MQ=255)

                                                          aCCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTg                                                                                        <  1:894635/125‑1 (MQ=255)

                                                          aCCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAAc                                                                         <  1:849592/140‑1 (MQ=255)

                                                          aCCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCATTATGAGCTGAGGGTTTGATTTAAc                                                                         >  2:968866/1‑140 (MQ=255)

                                                           ccGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACa                                                                        >  2:342819/1‑140 (MQ=255)

                                                              agagaACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAgg                                                                     >  1:109968/1‑140 (MQ=255)

                                                               gagaACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGc                                                                    >  1:266675/1‑140 (MQ=255)

                                                                agaACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCa                                                                   <  1:65308/140‑1 (MQ=255)

                                                                  aaCGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAg                                                                 <  1:709328/140‑1 (MQ=255)

                                                                     gggCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCAc                                                              <  1:756071/140‑1 (MQ=255)

                                                                     gggCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCAc                                                              <  1:506018/140‑1 (MQ=255)

                                                                      ggCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCAcc                                                             >  2:477551/1‑140 (MQ=255)

                                                                          ttCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTggg                                                         >  2:770036/1‑139 (MQ=255)

                                                                             tGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCgg                                                      >  1:912136/1‑140 (MQ=255)

                                                                              gTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTgg                                                          >  2:404246/1‑135 (MQ=255)

                                                                              gTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTgg                                                          <  1:404246/135‑1 (MQ=255)

                                                                                 ccAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGt                                                         >  2:493301/1‑133 (MQ=255)

                                                                                 ccAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGt                                                         <  1:493301/133‑1 (MQ=255)

                                                                                 ccAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATc                                                  >  1:644111/1‑140 (MQ=255)

                                                                                    gggCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGcc                                               <  1:969458/140‑1 (MQ=255)

                                                                                    gggCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGcc                                               <  2:982599/140‑1 (MQ=255)

                                                                                    gggCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGcc                                               <  2:379885/140‑1 (MQ=255)

                                                                                       cTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTg                                            >  1:284277/1‑140 (MQ=255)

                                                                                         tCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGtt                                          >  2:838234/1‑140 (MQ=255)

                                                                                               accaTGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGtt                                    >  1:179370/1‑140 (MQ=255)

                                                                                                 caTGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTtga                                  <  1:415287/140‑1 (MQ=255)

                                                                                                 caTGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTtga                                  <  1:443562/140‑1 (MQ=255)

                                                                                                 caTGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTtga                                  <  2:109968/140‑1 (MQ=255)

                                                                                                  aTGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTtgat                                 <  1:838234/140‑1 (MQ=255)

                                                                                                   tGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTtgatg                                >  1:555183/1‑140 (MQ=255)

                                                                                                          gtgAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAAt                         >  1:730841/1‑140 (MQ=255)

                                                                                                           tgAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTtgat                                 <  1:650209/131‑1 (MQ=255)

                                                                                                           tgAGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTtgat                                 >  2:650209/1‑131 (MQ=255)

                                                                                                             aGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAATTcc                      >  1:395704/1‑140 (MQ=255)

                                                                                                             aGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAATTcc                      >  1:1015132/1‑140 (MQ=255)

                                                                                                             aGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAATTcc                      <  2:1015132/140‑1 (MQ=255)

                                                                                                             aGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAATTcc                      >  2:942444/1‑140 (MQ=255)

                                                                                                             aGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAATTcc                      >  2:91122/1‑140 (MQ=255)

                                                                                                             aGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAATTcc                      >  1:378759/1‑140 (MQ=255)

                                                                                                             aGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAATTcc                      >  2:649947/1‑140 (MQ=255)

                                                                                                             aGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAATTcc                      <  2:644111/140‑1 (MQ=255)

                                                                                                             aGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAATTcc                      >  1:506274/1‑140 (MQ=255)

                                                                                                             aGCGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCATTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAATTcc                      <  1:968866/140‑1 (MQ=255)

                                                                                                               cGATCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAATTCCtt                    >  1:691581/1‑140 (MQ=255)

                                                                                                                 aTCCCGGCGGGGCAGTTGGCGCTCGCGACAAAACGCAAGGGTTACTCCCTCGCCACATCCTTTTGAGCTGAGGGGTTGATTTAACAAGGCCAACACCTGGGCTGGATCGCCCTGTTGCGGTTGATTGATAAATTCCTtgt                  >  2:467134/1‑140 (MQ=255)

                                                                                                                 aTCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAATTCCTtgt                  >  1:486227/1‑140 (MQ=255)

                                                                                                                  tCCCGGCGGGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAATTCCTtgtg                 >  1:957909/1‑140 (MQ=255)

                                                                                                                     cggcggAGCAGTTGGCGATCGCGACAATACGCAAAGCTTGCCCCATCGCCCCTTCCTTATGGGCTCGGGGCTTGATTTTAAAAGGCAACTACCTGGGCGGAATCGCCCTGTTGCAGTTGATGAATAAATTCCTTGTGaat              >  2:690830/1‑140 (MQ=255)

                                                                                                                       gcggGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAATTCCTTGTGaataa            <  2:730841/140‑1 (MQ=255)

                                                                                                                       gcggGGCAGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAATTCCTTGTGaataa            <  1:649947/140‑1 (MQ=255)

                                                                                                                              aGTTGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAATTCCTTGTGAATAATTTTGCg     <  1:510382/140‑1 (MQ=255)

                                                                                                                                 tGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAATTCCTTGTGAATAATTTTGCGaca  <  1:342819/140‑1 (MQ=255)

                                                                                                                                 tGGCGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAATTCCTTGTGAATAATTTTGCGaca  <  2:284277/140‑1 (MQ=255)

CGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGCCCTGTTGCAGTTGATGAATAAATTCCTTGTGAATAATTTTGCGACA  >  NC_000913/4092204‑4092472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A