Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,182,388	G→A	E382K (GAA→AAA)	rpoB →	RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,182,388	0	G	A	100.0%	274.0 / NA	77	E382K (GAA→AAA)	rpoB	RNA polymerase subunit beta
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (34/43); total (34/43)

TGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGA  >  NC_000913/4182251‑4182524

tGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTaaa                                                                                                                                        >  1:235651/1‑140 (MQ=255)

 gTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTaaaa                                                                                                                                       >  2:967436/1‑140 (MQ=255)

  ttCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAg                                                                                                                                      >  2:961115/1‑140 (MQ=255)

   tCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGc                                                                                                                                     <  1:929057/140‑1 (MQ=255)

   tCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGc                                                                                                                                     <  1:961115/140‑1 (MQ=255)

               tGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAAc                                                                                                                         <  1:793670/140‑1 (MQ=255)

               tGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAAc                                                                                                                         <  2:572462/140‑1 (MQ=255)

                 gATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCt                                                                                                                       >  1:162876/1‑140 (MQ=255)

                 gATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCt                                                                                                                       >  2:170954/1‑140 (MQ=255)

                  aTCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTg                                                                                                                      <  2:148695/140‑1 (MQ=255)

                    cACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGtt                                                                                                                    <  1:975675/140‑1 (MQ=255)

                    cACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCAAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGtt                                                                                                                    >  1:784150/1‑140 (MQ=255)

                      cGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGttct                                                                                                                  >  1:667508/1‑140 (MQ=255)

                      cGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGttct                                                                                                                  <  2:736584/140‑1 (MQ=255)

                       ggCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGttctt                                                                                                                 >  1:868271/1‑140 (MQ=255)

                         cccATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTtcttct                                                                                                               >  2:155425/1‑140 (MQ=255)

                           cATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTcc                                                                                                             >  1:387215/1‑140 (MQ=255)

                            atatatCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCg                                                                                                            >  2:322533/1‑140 (MQ=255)

                               tatCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAg                                                                                                         <  2:783859/140‑1 (MQ=255)

                               tatCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAg                                                                                                         <  2:834389/140‑1 (MQ=255)

                                atCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGa                                                                                                        <  2:162876/140‑1 (MQ=255)

                                atCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGa                                                                                                        <  2:977126/140‑1 (MQ=255)

                                atCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGa                                                                                                        <  1:151305/140‑1 (MQ=255)

                                     tGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGtt                                                                                                   >  2:830155/1‑140 (MQ=255)

                                          ccTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGAc                                                                                              <  1:830155/140‑1 (MQ=255)

                                          ccTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGAc                                                                                              <  1:560221/140‑1 (MQ=255)

                                          ccTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGAc                                                                                              <  1:76743/140‑1 (MQ=255)

                                           cTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACt                                                                                             >  2:1001970/1‑140 (MQ=255)

                                             tACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTg                                                                                           >  2:736437/1‑140 (MQ=255)

                                             tACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTg                                                                                           >  2:339009/1‑140 (MQ=255)

                                                 tgtCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTg                                                                                       >  2:638786/1‑140 (MQ=255)

                                                    cGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCgg                                                                                    >  2:499096/1‑140 (MQ=255)

                                                     gACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGt                                                                                   <  2:868271/140‑1 (MQ=255)

                                                     gACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGt                                                                                   <  2:235651/140‑1 (MQ=255)

                                                      aCCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGtt                                                                                  >  2:877319/1‑140 (MQ=255)

                                                       cccAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTg                                                                                 <  2:74977/140‑1 (MQ=255)

                                                       cccAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTg                                                                                 <  1:852442/140‑1 (MQ=255)

                                                       cccAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTg                                                                                 <  1:967436/140‑1 (MQ=255)

                                                        ccAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACATGTCTGCGGTTgg                                                                                <  2:783826/140‑1 (MQ=255)

                                                           aCTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCg                                                                             >  1:72511/1‑140 (MQ=255)

                                                            cTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGt                                                                            >  2:568332/1‑140 (MQ=255)

                                                             tAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTa                                                                           <  1:684582/140‑1 (MQ=255)

                                                             tAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTa                                                                           <  1:174809/140‑1 (MQ=255)

                                                                      tCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCa                                                                  >  2:578101/1‑140 (MQ=255)

                                                                      tCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCa                                                                  >  1:29168/1‑140 (MQ=255)

                                                                       cTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCaa                                                                 <  1:639665/140‑1 (MQ=255)

                                                                        tGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAAc                                                                >  2:454146/1‑140 (MQ=255)

                                                                         gAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAAcc                                                               >  1:858101/1‑140 (MQ=255)

                                                                             gcACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAATCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTtc                                                           >  1:522635/1‑140 (MQ=255)

                                                                                 tGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTctg                                                       >  2:229175/1‑140 (MQ=255)

                                                                                 tGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTctg                                                       >  1:995062/1‑140 (MQ=255)

                                                                                 tGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTctg                                                       >  2:134300/1‑140 (MQ=255)

                                                                                      gAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTgcg                                                  <  2:193794/140‑1 (MQ=255)

                                                                                        aaTCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGcgcg                                                <  1:322533/140‑1 (MQ=255)

                                                                                        aaTCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGcgcg                                                <  1:546665/140‑1 (MQ=255)

                                                                                           cTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCgaag                                             >  1:554394/1‑140 (MQ=255)

                                                                                             aCCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCgaagaa                                           >  1:293094/1‑140 (MQ=255)

                                                                                             aCCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCgaagaa                                           >  2:383533/1‑140 (MQ=255)

                                                                                             aCCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCgaagaa                                           >  2:745747/1‑140 (MQ=255)

                                                                                             aCCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCgaagaa                                           >  1:25151/1‑140 (MQ=255)

                                                                                             aCCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCgaagaa                                           >  1:502628/1‑140 (MQ=255)

                                                                                             aCCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCgaagaa                                           >  1:162312/1‑140 (MQ=255)

                                                                                              ccGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGaaa                                          >  2:401768/1‑140 (MQ=255)

                                                                                                  atgatgCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGa                                      <  2:25151/140‑1 (MQ=255)

                                                                                                     atgCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAgg                                   <  2:502628/140‑1 (MQ=255)

                                                                                                     atgCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAgg                                   <  2:29168/140‑1 (MQ=255)

                                                                                                       gcgcCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGtt                                 <  1:499096/140‑1 (MQ=255)

                                                                                                         gcCCTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTcc                               <  2:667508/140‑1 (MQ=255)

                                                                                                          cccTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCg                              <  2:387215/140‑1 (MQ=255)

                                                                                                            cTGGCGAGCCGCCGACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGt                            <  1:134300/140‑1 (MQ=255)

                                                                                                                        cgACTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCaaa                <  2:72511/140‑1 (MQ=255)

                                                                                                                          aCTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAga              <  1:1001970/140‑1 (MQ=255)

                                                                                                                          aCTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAga              <  1:736437/140‑1 (MQ=255)

                                                                                                                           cTCGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAgac             <  1:383533/140‑1 (MQ=255)

                                                                                                                             cGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAgacga           <  1:461316/140‑1 (MQ=255)

                                                                                                                             cGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAgacga           <  1:745747/140‑1 (MQ=255)

                                                                                                                             cGTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAgacga           <  1:568332/140‑1 (MQ=255)

                                                                                                                              gTGAAGCAGCTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAgacgac          <  1:724249/140‑1 (MQ=255)

                                                                                                                                      gcTAAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGa  <  1:339009/140‑1 (MQ=255)

TGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGA  >  NC_000913/4182251‑4182524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A