Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 155 60.3 2039550 99.3% 2025273 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,493,576 C→T M277I (ATG→ATA)  idnD ← L‑idonate 5‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,493,5760CT97.7% 309.5 / ‑1.1 88M277I (ATG→ATA) idnDL‑idonate 5‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (42/44);  minor base A (1/1);  total (43/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01

ATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCT  >  NC_000913/4493453‑4493708
                                                                                                                           |                                                                                                                                    
atatTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTg                                                                                                                      <  1:377387/140‑1 (MQ=255)
   ttCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCa                                                                                                                   >  2:654498/1‑140 (MQ=255)
   ttCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCa                                                                                                                   >  1:390784/1‑140 (MQ=255)
      aGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATcg                                                                                                                >  1:784034/1‑140 (MQ=255)
       gCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATcgc                                                                                                               <  2:979319/140‑1 (MQ=255)
       gCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATcgc                                                                                                               <  2:267736/140‑1 (MQ=255)
        cACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATcgcg                                                                                                              <  2:861955/140‑1 (MQ=255)
        cACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATcgcg                                                                                                              >  2:778375/1‑140 (MQ=255)
        cACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATcgcg                                                                                                              >  2:650195/1‑140 (MQ=255)
        cACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATcgcg                                                                                                              <  1:159269/140‑1 (MQ=255)
        cACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATcgcg                                                                                                              >  2:290569/1‑140 (MQ=255)
        cACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATcgcg                                                                                                              >  2:155484/1‑140 (MQ=255)
        cACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATcgcg                                                                                                              <  1:290569/140‑1 (MQ=255)
         aCTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCgcgc                                                                                                             <  2:784034/140‑1 (MQ=255)
          cTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATcgcg                                                                                                              >  2:111590/1‑138 (MQ=255)
          cTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATcgcg                                                                                                              <  1:111590/138‑1 (MQ=255)
           tCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACTCTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCt                                                                                                           >  2:836566/1‑140 (MQ=255)
           tCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCt                                                                                                           >  1:907053/1‑140 (MQ=255)
           tCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCt                                                                                                           >  2:134817/1‑140 (MQ=255)
            cAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACTCTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTc                                                                                                          <  1:836566/140‑1 (MQ=255)
            cAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTc                                                                                                          <  2:33552/140‑1 (MQ=255)
            cAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCCCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTc                                                                                                          <  2:457995/140‑1 (MQ=255)
             aGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTcc                                                                                                         >  1:1009372/1‑140 (MQ=255)
             aGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTcc                                                                                                         <  1:155484/140‑1 (MQ=255)
             aGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTcc                                                                                                         >  1:199053/1‑140 (MQ=255)
             aGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTcc                                                                                                         <  1:608657/140‑1 (MQ=255)
             aGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTAATCATTGGGAATTCTGCCATCGCGCCTc                                                                                                          <  1:231650/139‑1 (MQ=255)
             aGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTAATCATTGGGAATTCTGCCATCGCGCCTc                                                                                                          >  2:231650/1‑139 (MQ=255)
                 aaGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCAta                                                                                                     <  1:650195/140‑1 (MQ=255)
                     cAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCAtaccta                                                                                                 >  1:376513/1‑140 (MQ=255)
                      aGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCAtacctac                                                                                                >  1:132660/1‑140 (MQ=255)
                       gTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCAtacctacc                                                                                               >  1:868299/1‑140 (MQ=255)
                       gTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCAtacctacc                                                                                               >  2:512318/1‑140 (MQ=255)
                       gTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCAtacctacc                                                                                               >  1:155967/1‑140 (MQ=255)
                       gTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCAtacctacc                                                                                               >  1:486284/1‑140 (MQ=255)
                       gTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCAtacctacc                                                                                               >  2:849088/1‑140 (MQ=255)
                       gTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCAtacctacc                                                                                               >  2:924065/1‑140 (MQ=255)
                        tGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTcc                                                                                                         <  1:593487/129‑1 (MQ=255)
                        tGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTcc                                                                                                         >  2:593487/1‑129 (MQ=255)
                         ggATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCAtacct                                                                                                  >  1:1015754/1‑135 (MQ=255)
                         ggATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCAtacct                                                                                                  <  2:1015754/135‑1 (MQ=255)
                         ggATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTg                                                                                             <  2:964019/140‑1 (MQ=255)
                         ggATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTg                                                                                             <  2:876953/140‑1 (MQ=255)
                         ggATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTg                                                                                             >  1:416128/1‑140 (MQ=255)
                         ggATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTg                                                                                             <  1:884383/140‑1 (MQ=255)
                               aTAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCAt                                                                                       >  2:553261/1‑140 (MQ=255)
                                 aaCGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTa                                                                                     <  2:199053/140‑1 (MQ=255)
                                    gCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTAcgc                                                                                  >  2:795129/1‑140 (MQ=255)
                                        ttCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCcgcg                                                                              <  2:376513/140‑1 (MQ=255)
                                          cGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTg                                                                            <  2:486284/140‑1 (MQ=255)
                                            ccagccaTGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCa                                                                          <  1:52191/140‑1 (MQ=255)
                                            ccagccaTGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACCTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCa                                                                          <  2:605474/140‑1 (MQ=255)
                                             cagccaTGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCAc                                                                         >  2:382590/1‑140 (MQ=255)
                                                ccaTGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAg                                                                      <  1:512318/140‑1 (MQ=255)
                                                 caTGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGt                                                                     <  1:382590/140‑1 (MQ=255)
                                                 caTGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGt                                                                     <  2:155967/140‑1 (MQ=255)
                                                 caTGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGt                                                                     <  1:924065/140‑1 (MQ=255)
                                                       acTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTc                                                               <  2:907053/140‑1 (MQ=255)
                                                       acTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTc                                                               <  1:553261/140‑1 (MQ=255)
                                                         tGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCa                                                             >  2:187979/1‑140 (MQ=255)
                                                           cGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGa                                                           <  2:343195/140‑1 (MQ=255)
                                                           cGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGa                                                           <  2:831913/140‑1 (MQ=255)
                                                           cGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGa                                                           >  1:831913/1‑140 (MQ=255)
                                                            ggTATTAAATTCGCTGGTAAGACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGAc                                                          <  1:746595/140‑1 (MQ=255)
                                                              tATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAg                                                        <  1:428841/140‑1 (MQ=255)
                                                                     ttCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCa                                                 <  1:187979/140‑1 (MQ=255)
                                                                     ttCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCa                                                 >  2:93284/1‑140 (MQ=255)
                                                                     ttCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCa                                                 <  2:1009372/140‑1 (MQ=255)
                                                                       cGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACt                                               >  2:559025/1‑140 (MQ=255)
                                                                        gCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGa                                                           <  1:481144/127‑1 (MQ=255)
                                                                        gCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGa                                                           >  2:481144/1‑127 (MQ=255)
                                                                        gCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACtg                                              >  2:768651/1‑140 (MQ=255)
                                                                          tGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACTAGTGACCTCCAGACAGGTATTCACtga                                             <  1:845590/139‑1 (MQ=255)
                                                                          tGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACTAGTGACCTCCAGACAGGTATTCACtga                                             >  2:845590/1‑139 (MQ=255)
                                                                          tGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACtgat                                            >  2:670038/1‑140 (MQ=255)
                                                                          tGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACtgat                                            <  1:93284/140‑1 (MQ=255)
                                                                             tAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGaa                                         >  1:655282/1‑140 (MQ=255)
                                                                              aaaaCGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAg                                        <  1:645624/140‑1 (MQ=255)
                                                                                        gagCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGa                              <  1:134817/140‑1 (MQ=255)
                                                                                          gCCTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGaca                            <  2:459992/140‑1 (MQ=255)
                                                                                           ccTCTGAGTGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGacac                           >  1:953942/1‑140 (MQ=255)
                                                                                           ccTCTGAGAGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGacac                           >  2:811689/1‑140 (MQ=255)
                                                                                               tGAGTGAAATCTCCTTACCGATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCa                       >  2:410683/1‑140 (MQ=255)
                                                                                                   tGAAATCTCCTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGc                   <  2:132660/140‑1 (MQ=255)
                                                                                                           ccTTACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCg           >  2:848382/1‑140 (MQ=255)
                                                                                                             ttACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGaa         >  1:299462/1‑140 (MQ=255)
                                                                                                              tACCAATCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGaaa        <  1:559025/140‑1 (MQ=255)
                                                                                                                    tCAACGTTATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCt  >  1:160685/1‑140 (MQ=255)
                                                                                                                           |                                                                                                                                    
ATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCT  >  NC_000913/4493453‑4493708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A