Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F28 I1 R1 151 87.4 2971636 99.2% 2947862 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,297,114 (C)7→8 coding (531/624 nt) ccmA ← heme trafficking system ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,297,1071.C95.6% 150.4 / ‑0.1 45coding (538/624 nt)ccmAheme trafficking system ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (19/24);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑CCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGACGCG  >  NC_000913/2296970‑2297239
                                                                                                                                          |                                                                                                                                    
cgcTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑cc                                                                                                                                    >  1:914369/1‑140 (MQ=255)
  cTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTCCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑cccc                                                                                                                                  >  1:229707/1‑140 (MQ=255)
     tGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑ccccccc                                                                                                                               <  2:726953/140‑1 (MQ=255)
       gCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑cccccccct                                                                                                                             <  1:670540/140‑3 (MQ=255)
       gCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑cccccccct                                                                                                                             <  1:36094/140‑3 (MQ=255)
        cGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑cccccccctg                                                                                                                            <  2:331510/140‑4 (MQ=255)
        cGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑cccccccctg                                                                                                                            <  1:1328821/140‑4 (MQ=255)
               cTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑ccccccc                                                                                                                               >  2:787883/1‑130 (MQ=255)
               cTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑ccccccc                                                                                                                               <  1:787883/130‑1 (MQ=255)
               cTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTCCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑cccccccct                                                                                                                             <  2:576339/132‑3 (MQ=255)
               cTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTCCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑cccccccct                                                                                                                             >  1:576339/1‑130 (MQ=255)
               cTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGACGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTa                                                                                                                      >  1:105298/1‑140 (MQ=255)
                   acGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTAtgct                                                                                                                  <  2:739241/140‑1 (MQ=255)
                   acGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTAtgct                                                                                                                  <  1:628630/140‑1 (MQ=255)
                       aGCTCAAGACGGAAAATGCGCCAGAACATCATGTGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTgcgc                                                                                                              >  2:220909/1‑140 (MQ=255)
                       aGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTgcgc                                                                                                              >  2:1267134/1‑140 (MQ=255)
                       aGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑CCCCCCCTGCTCCGTAtgct                                                                                                                  <  2:999605/135‑1 (MQ=255)
                       aGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑CCCCCCCTGCTCCGTAtgct                                                                                                                  >  1:999605/1‑135 (MQ=255)
                        gCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTTGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGcc                                                                                                             <  2:812336/140‑1 (MQ=255)
                        gCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGcc                                                                                                             >  1:634104/1‑140 (MQ=255)
                        gCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGcc                                                                                                             >  1:1232580/1‑140 (MQ=255)
                            aaGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATAc                                                                                                         >  2:957175/1‑140 (MQ=255)
                            aaGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTCCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATAc                                                                                                         <  2:229707/140‑1 (MQ=255)
                               aCGGAAAATGCGCCAGAACATCATGTGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCt                                                                                                      <  1:220909/140‑1 (MQ=255)
                                         cgcCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACg                                                                                            >  1:1426588/1‑140 (MQ=255)
                                            cAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATc                                                                                         >  1:1254185/1‑140 (MQ=255)
                                                aCATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGaca                                                                                     <  1:709647/140‑1 (MQ=255)
                                                aCATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGaca                                                                                     <  2:914369/140‑1 (MQ=255)
                                                   tcatGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCg                                                                                  <  1:112590/140‑1 (MQ=255)
                                                      tGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTa                                                                               >  1:350579/1‑140 (MQ=255)
                                                         ggCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACg                                                                            <  2:1426588/140‑1 (MQ=255)
                                                            ccTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCa                                                                         >  2:846591/1‑140 (MQ=255)
                                                              tCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAAt                                                                       >  2:136140/1‑140 (MQ=255)
                                                                gTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATcg                                                                     <  1:1237299/140‑1 (MQ=255)
                                                                gTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATcg                                                                     <  1:660731/140‑1 (MQ=255)
                                                                gTTTGCGTCAGTGAAATGCGACGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATcg                                                                     <  2:105298/140‑1 (MQ=255)
                                                                 tttGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATcgc                                                                    >  1:1036808/1‑140 (MQ=255)
                                                                          gTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAgg                                                           <  1:846591/140‑1 (MQ=255)
                                                                          gTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAgg                                                           <  2:350579/140‑1 (MQ=255)
                                                                             aaaTGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtc                                                        <  2:11570/140‑1 (MQ=255)
                                                                                 gcggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtcgtcg                                                    <  2:1232580/140‑1 (MQ=255)
                                                                                 gcggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtcgtcg                                                    <  2:634104/140‑1 (MQ=255)
                                                                                  cggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGa                                                   <  1:1212428/140‑1 (MQ=255)
                                                                                  cggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGa                                                   <  1:957175/140‑1 (MQ=255)
                                                                                   ggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGa                                                   <  1:1438478/139‑1 (MQ=255)
                                                                                   ggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGa                                                   >  2:1438478/1‑139 (MQ=255)
                                                                                             aCTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAAc                                        <  1:698162/140‑1 (MQ=255)
                                                                                                                  cTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGa                   >  2:451884/1‑140 (MQ=255)
                                                                                                                   tggtggGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGAc                  >  1:1302057/1‑140 (MQ=255)
                                                                                                                           aGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTaaa          <  2:1254185/140‑1 (MQ=255)
                                                                                                                            gTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGAcgcg               <  2:904623/134‑1 (MQ=255)
                                                                                                                            gTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGAcgcg               >  1:904623/1‑134 (MQ=255)
                                                                                                                            gTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAg         >  1:1191479/1‑140 (MQ=255)
                                                                                                                               aGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGa      >  1:667472/1‑140 (MQ=255)
                                                                                                                                   tCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGAcgcg  <  1:136140/140‑1 (MQ=255)
                                                                                                                                   tCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGAcgcg  >  2:17592/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                    
CGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑CCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGACGCG  >  NC_000913/2296970‑2297239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A