Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	151	87.4	2971636	99.2%	2947862	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,660,213	T→C	N26S (AAC→AGC)	iscU ←	scaffold protein for iron‑sulfur cluster assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,660,213	0	T	C	100.0%	203.9 / NA	57	N26S (AAC→AGC)	iscU	scaffold protein for iron‑sulfur cluster assembly
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (34/23); total (34/23)

GAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGCTGTTCAGATC  >  NC_000913/2660078‑2660349

gAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCtctc                                                                                                                                      >  2:976274/1‑140 (MQ=255)

  gCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCtcgt                                                                                                                                    >  2:433361/1‑140 (MQ=255)

    tGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCtcgtcg                                                                                                                                  >  2:646573/1‑140 (MQ=255)

    tGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCtcgtcg                                                                                                                                  >  2:1299579/1‑140 (MQ=255)

           gATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTtgttgt                                                                                                                           >  2:440092/1‑140 (MQ=255)

                cGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAg                                                                                                                      >  2:649877/1‑140 (MQ=255)

                                aGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTAcgcg                                                                                                      <  1:976274/140‑1 (MQ=255)

                                aGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTAcgcg                                                                                                      <  1:646573/140‑1 (MQ=255)

                                 gTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCgg                                                                                                     <  1:440092/140‑1 (MQ=255)

                                   tttAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCAACACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGAt                                                                                                   <  1:342515/140‑1 (MQ=255)

                                      aaaaCGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATtct                                                                                                >  2:108476/1‑140 (MQ=255)

                                            cgcgTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAAt                                                                                          <  1:946153/140‑1 (MQ=255)

                                                          tACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACtttt                                                                            >  2:1045085/1‑140 (MQ=255)

                                                           aCCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACttttt                                                                           >  2:169338/1‑140 (MQ=255)

                                                           aCCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACttttt                                                                           >  2:190767/1‑140 (MQ=255)

                                                           aCCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACttttt                                                                           >  2:113354/1‑140 (MQ=255)

                                                           aCCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACttttt                                                                           >  2:230581/1‑140 (MQ=255)

                                                           aCCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACttttt                                                                           >  2:371176/1‑140 (MQ=255)

                                                           aCCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACttttt                                                                           <  2:410906/140‑1 (MQ=255)

                                                             cTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCg                                                                         >  2:203224/1‑140 (MQ=255)

                                                             cTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCg                                                                         >  2:1252750/1‑140 (MQ=255)

                                                                cATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTg                                                                      >  2:1472455/1‑140 (MQ=255)

                                                                  tCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTa                                                                    <  1:1472455/140‑1 (MQ=255)

                                                                  tCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTa                                                                    >  2:212202/1‑140 (MQ=255)

                                                                  tCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTa                                                                    <  1:1037522/140‑1 (MQ=255)

                                                                  tCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTa                                                                    <  1:108476/140‑1 (MQ=255)

                                                                    gTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAg                                                                  <  1:649877/140‑1 (MQ=255)

                                                                     ttGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGc                                                                 >  2:528036/1‑140 (MQ=255)

                                                                     ttGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGc                                                                 >  2:539826/1‑140 (MQ=255)

                                                                        aCTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCAt                                                              >  2:290654/1‑140 (MQ=255)

                                                                           ttAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTCTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATtat                                                           <  1:1299579/140‑1 (MQ=255)

                                                                             aaTCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATaa                                                         >  2:189197/1‑140 (MQ=255)

                                                                                   cAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATtctc                                                   >  2:475730/1‑140 (MQ=255)

                                                                                   cAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATtctc                                                   >  2:141293/1‑140 (MQ=255)

                                                                                    aaCTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAg                                                                  >  2:150941/1‑124 (MQ=255)

                                                                                    aaCTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAg                                                                  <  1:150941/124‑1 (MQ=255)

                                                                                    aaCTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTcc                                                  >  2:304724/1‑140 (MQ=255)

                                                                                     aCTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCt                                                 <  1:1045085/140‑1 (MQ=255)

                                                                                     aCTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCt                                                 <  1:475730/140‑1 (MQ=255)

                                                                                         catcaCGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCt                                                 <  1:106682/136‑1 (MQ=255)

                                                                                         catcaCGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCt                                                 >  2:106682/1‑136 (MQ=255)

                                                                                               gTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATa                                       <  1:212202/140‑1 (MQ=255)

                                                                                               gTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATa                                       <  1:290654/140‑1 (MQ=255)

                                                                                               gTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATa                                       <  1:1252750/140‑1 (MQ=255)

                                                                                                tCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCACTGTAAGCCATTATAAATTCTCCTGATTCCGATAc                                      >  2:1027665/1‑140 (MQ=255)

                                                                                                 cGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATAcc                                     >  2:611558/1‑140 (MQ=255)

                                                                                                 cGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATAcc                                     >  2:324200/1‑140 (MQ=255)

                                                                                                 cGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATAcc                                     <  1:189197/140‑1 (MQ=255)

                                                                                                             gTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACTGATTAAtgatga                         >  2:365708/1‑140 (MQ=255)

                                                                                                              tGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAg                        >  2:903212/1‑140 (MQ=255)

                                                                                                               gCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGc                       <  1:528036/140‑1 (MQ=255)

                                                                                                               gCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGc                       <  1:539826/140‑1 (MQ=255)

                                                                                                               gCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGc                       <  1:611558/140‑1 (MQ=255)

                                                                                                                          gCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGc            >  2:1164693/1‑140 (MQ=255)

                                                                                                                          gCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGc            >  2:475749/1‑140 (MQ=255)

                                                                                                                              cTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGCTGtt        <  1:433361/140‑1 (MQ=255)

                                                                                                                                    aCGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGCTGTTCAGATc  >  2:809929/1‑140 (MQ=255)

GAGCTGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGCTGTTCAGATC  >  NC_000913/2660078‑2660349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A