Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 183 98.6 3427438 96.9% 3321187 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,892,882 (C)6→7 coding (354/1911 nt) yoaA ← putative 5' to 3' DNA helicase implicated in DNA repair

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,892,8761.C100.0% 325.4 / NA 87G120G (GGC→GGG) yoaAputative 5' to 3' DNA helicase implicated in DNA repair
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (42/45);  total (42/45)

TGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑G‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTG  >  NC_000913/1892743‑1893010
                                                                                                                                       |                                                                                                                                      
tGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑G‑cccccc                                                                                                                                  >  2:1642483/1‑140 (MQ=255)
  gTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑G‑cccccccg                                                                                                                                <  2:1025644/140‑3 (MQ=255)
   tGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑G‑cccccccgc                                                                                                                               >  2:587947/1‑137 (MQ=255)
   tGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑G‑cccccccgc                                                                                                                               >  2:1078128/1‑137 (MQ=255)
    gCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑G‑cccccccgcc                                                                                                                              <  2:1332335/140‑5 (MQ=255)
     cTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑G‑cccccccgcca                                                                                                                             >  2:36400/1‑135 (MQ=255)
       ggTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAgc                                                                                                                            >  1:528219/1‑140 (MQ=255)
       ggTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAgc                                                                                                                            <  2:528219/140‑1 (MQ=255)
        gTGACTAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAgcg                                                                                                                           >  1:430487/1‑140 (MQ=255)
            ccAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGCCCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCctg                                                                                                                       <  2:227243/140‑1 (MQ=255)
             cAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCctgc                                                                                                                      >  2:698393/1‑140 (MQ=255)
             cAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCctgc                                                                                                                      <  2:1422609/140‑1 (MQ=255)
             cAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCctgc                                                                                                                      <  2:349786/140‑1 (MQ=255)
               gCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCctgctg                                                                                                                    >  2:69222/1‑140 (MQ=255)
                cGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGt                                                                                                                   >  2:1678090/1‑140 (MQ=255)
                     aCGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTCCCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCgag                                                                                                              <  2:1318646/140‑1 (MQ=255)
                     aCGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCgag                                                                                                              <  2:1679081/140‑1 (MQ=255)
                          tgtgAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGtt                                                                                                         <  1:456062/140‑1 (MQ=255)
                               aTCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAgg                                                                                                    <  2:90088/140‑1 (MQ=255)
                               aTCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAgg                                                                                                    <  1:1678090/140‑1 (MQ=255)
                                 cTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCa                                                                                                  <  2:1618637/140‑1 (MQ=255)
                                   tCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCaga                                                                                                >  1:711828/1‑140 (MQ=255)
                                   tCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCaga                                                                                                >  2:764990/1‑140 (MQ=255)
                                               aCGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAgc                                                                                    <  1:232509/140‑1 (MQ=255)
                                               aCGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAgc                                                                                    <  1:587947/140‑1 (MQ=255)
                                               aCGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAgc                                                                                    <  2:1269446/140‑1 (MQ=255)
                                               aCGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAgc                                                                                    <  1:1607292/140‑1 (MQ=255)
                                               aCGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAgc                                                                                    <  2:1646664/140‑1 (MQ=255)
                                                     gTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCtt                                                                              >  2:1606292/1‑140 (MQ=255)
                                                     gTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACAGGCAAATC‑GCCCCCCCGCCGGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTGGTTTGAGCGACCtt                                                                              >  2:276096/1‑140 (MQ=255)
                                                               cACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGGCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAgcgc                                                                    >  2:1474017/1‑140 (MQ=255)
                                                               cACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAgcgc                                                                    >  1:1713694/1‑140 (MQ=255)
                                                               cACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAgcgc                                                                    >  1:565255/1‑140 (MQ=255)
                                                               cACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAgcgc                                                                    >  2:1006776/1‑140 (MQ=255)
                                                                aCCATCGACTGTTTGATTAGCCCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAgc                                                                                    <  2:1649452/123‑1 (MQ=255)
                                                                aCCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAgc                                                                                    >  1:1649452/1‑123 (MQ=255)
                                                                  cATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCAc                                                                 <  2:1378710/140‑1 (MQ=255)
                                                                   aTCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACg                                                                >  2:99724/1‑140 (MQ=255)
                                                                    tCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGt                                                               <  1:99724/140‑1 (MQ=255)
                                                                     cGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGtt                                                              >  2:659476/1‑140 (MQ=255)
                                                                     cGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGtt                                                              >  2:279935/1‑140 (MQ=255)
                                                                       aCTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACg                                                                <  1:255575/136‑1 (MQ=255)
                                                                       aCTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACg                                                                >  2:255575/1‑136 (MQ=255)
                                                                       aCTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGc                                                            <  1:1006776/140‑1 (MQ=255)
                                                                       aCTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGc                                                            <  1:764990/140‑1 (MQ=255)
                                                                       aCTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGc                                                            >  2:72073/1‑140 (MQ=255)
                                                                       aCTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGc                                                            >  2:204246/1‑140 (MQ=255)
                                                                           tttGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGc                                                            >  1:19409/1‑136 (MQ=255)
                                                                           tttGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGc                                                            <  2:19409/136‑1 (MQ=255)
                                                                           tttGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGt                                                        <  1:694451/140‑1 (MQ=255)
                                                                            ttGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTCCCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGta                                                       <  1:831122/140‑1 (MQ=255)
                                                                            ttGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGta                                                       <  1:1078128/140‑1 (MQ=255)
                                                                                ttAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATAtt                                                   >  2:1144387/1‑140 (MQ=255)
                                                                                ttAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATAtt                                                   >  2:1040861/1‑140 (MQ=255)
                                                                                   gACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCa                                                <  1:698393/140‑1 (MQ=255)
                                                                                     ccAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAAt                                              <  2:233729/140‑1 (MQ=255)
                                                                                     ccAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAAt                                              >  2:200592/1‑140 (MQ=255)
                                                                                     ccAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAAt                                              >  2:1645199/1‑140 (MQ=255)
                                                                                     ccAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAAt                                              >  1:233729/1‑140 (MQ=255)
                                                                                     ccAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAAt                                              >  1:236435/1‑140 (MQ=255)
                                                                                     ccAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAAt                                              <  1:200592/140‑1 (MQ=255)
                                                                                     ccAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAAt                                              >  2:1388732/1‑140 (MQ=255)
                                                                                     ccAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAAt                                              <  1:279935/140‑1 (MQ=255)
                                                                                      cAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATg                                             >  1:52470/1‑140 (MQ=255)
                                                                                      cAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATg                                             <  1:1388732/140‑1 (MQ=255)
                                                                                              cagcagGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTgag                                     >  1:1338832/1‑140 (MQ=255)
                                                                                              cagcagGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTgag                                     >  1:138196/1‑140 (MQ=255)
                                                                                              cagcagGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTgag                                     >  2:476101/1‑140 (MQ=255)
                                                                                              cagcagGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTgag                                     >  1:500351/1‑140 (MQ=255)
                                                                                                gcagGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGAc                                   <  2:565255/140‑1 (MQ=255)
                                                                                                gcagGATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGAc                                   <  1:72073/140‑1 (MQ=255)
                                                                                                    gATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAAt                                              <  1:1262261/125‑1 (MQ=255)
                                                                                                    gATCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAAt                                              >  2:1262261/1‑125 (MQ=255)
                                                                                                      tCACATCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTgg                             <  2:1198048/140‑1 (MQ=255)
                                                                                                           tCGCTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAAt                        <  1:1005822/140‑1 (MQ=255)
                                                                                                              tttAAGATTTGTTCCGGCAGATCTC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATcgc                     <  2:292248/139‑1 (MQ=255)
                                                                                                              cTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTgaga                                    <  1:481442/125‑1 (MQ=255)
                                                                                                              cTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTgaga                                    >  2:481442/1‑125 (MQ=255)
                                                                                                              cTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATcgc                     >  1:292248/1‑140 (MQ=255)
                                                                                                              cTTAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATcgc                     <  1:69222/140‑1 (MQ=255)
                                                                                                               ttAAGATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATcgcg                    >  1:210671/1‑140 (MQ=255)
                                                                                                                   gATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTg                <  2:52470/140‑1 (MQ=255)
                                                                                                                   gATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTg                <  2:210671/140‑1 (MQ=255)
                                                                                                                   gATTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTg                <  1:1645199/140‑1 (MQ=255)
                                                                                                                    aTTTGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGt               <  2:1338832/140‑1 (MQ=255)
                                                                                                                    aTTTCTACCGGGAGATC‑GCCCCCCCGACAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGt               <  1:276096/140‑1 (MQ=255)
                                                                                                                      ttGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTag             >  2:152955/1‑140 (MQ=255)
                                                                                                                       tGTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTaga            >  2:1601461/1‑140 (MQ=255)
                                                                                                                        gTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCgg                   >  1:1367507/1‑132 (MQ=255)
                                                                                                                        gTACCGGCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCgg                   <  2:1367507/132‑1 (MQ=255)
                                                                                                                             ggCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATcg                      <  2:1052129/124‑1 (MQ=255)
                                                                                                                             ggCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATcg                      >  1:1052129/1‑124 (MQ=255)
                                                                                                                              gCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATc     >  1:564599/1‑140 (MQ=255)
                                                                                                                              gCAGATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATc     >  1:1034686/1‑140 (MQ=255)
                                                                                                                                 gATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTg  <  1:1040861/140‑1 (MQ=255)
                                                                                                                                 gATC‑GCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTg  <  1:1144387/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
TGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATC‑G‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTG  >  NC_000913/1892743‑1893010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A