Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 183 98.6 3427438 96.9% 3321187 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,981,454 (T)6→7 coding (159/1425 nt) otsA ← trehalose‑6‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,981,4481.T97.5% 285.5 / ‑0.8 81coding (165/1425 nt)otsAtrehalose‑6‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base T (43/36);  total (44/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01

CAGGACGCTGAAATTGCACCAGATCGAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACC‑TTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAAT  >  NC_000913/1981309‑1981582
                                                                                                                                            |                                                                                                                                      
caggACGCTGAAATTGCACCAGATCGAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCAcc                                                                                                                                         >  2:619330/1‑140 (MQ=255)
   gACGCTGAAATTGCACCAGATCGAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACC‑ttt                                                                                                                                     >  1:411651/1‑140 (MQ=255)
   gACGCTGAAATTGCACCAGATCGAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACC‑ttt                                                                                                                                     >  2:1433643/1‑140 (MQ=255)
   gACGCTGAAATTGCACCAGATCGAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACC‑ttt                                                                                                                                     >  1:1587959/1‑140 (MQ=255)
    aCGCTGAAATTGCACCAGATCGAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACC‑tttt                                                                                                                                    >  2:1059813/1‑140 (MQ=255)
        tGAAATTGCACCAGATCGAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACC‑ttttttta                                                                                                                                >  1:1660629/1‑138 (MQ=255)
          aaaTTGCACCAGATCGAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACC‑tttttttagc                                                                                                                              <  2:617677/140‑5 (MQ=255)
              tGCACCAAATCGAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCt                                                                                                                           <  1:276313/140‑1 (MQ=255)
                cACCAGATCGAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGa                                                                                                                         <  1:729952/140‑1 (MQ=255)
                  ccAGATCGAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATc                                                                                                                       <  1:1059813/140‑1 (MQ=255)
                      aTCGAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCa                                                                                                                   >  1:128845/1‑140 (MQ=255)
                       tCGAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCAt                                                                                                                  >  1:610370/1‑140 (MQ=255)
                         gAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTc                                                                                                                <  1:1433643/140‑1 (MQ=255)
                          aGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTcc                                                                                                               <  1:619330/140‑1 (MQ=255)
                          aGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTcc                                                                                                               <  1:1090635/140‑1 (MQ=255)
                          aGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTcc                                                                                                               >  1:1047499/1‑140 (MQ=255)
                             cGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTg                                                                                                            <  2:405686/140‑1 (MQ=255)
                                tAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGttt                                                                                                         >  2:1699709/1‑140 (MQ=255)
                                            ggCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGc                                                                                             >  1:693780/1‑140 (MQ=255)
                                             gCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGcc                                                                                            <  1:1699709/140‑1 (MQ=255)
                                                       ggCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGt                                                                                  >  2:1415650/1‑140 (MQ=255)
                                                         cATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTcc                                                                                >  2:1347354/1‑140 (MQ=255)
                                                                   ttGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCt                                                                      >  1:145599/1‑140 (MQ=255)
                                                                   ttGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCt                                                                      >  1:480330/1‑140 (MQ=255)
                                                                   ttGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCt                                                                      >  2:206369/1‑140 (MQ=255)
                                                                   ttGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCt                                                                      >  1:937977/1‑140 (MQ=255)
                                                                      gTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTc                                                                   <  1:783010/140‑1 (MQ=255)
                                                                      gTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTc                                                                   >  1:934738/1‑140 (MQ=255)
                                                                        tGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAg                                                                 >  2:363767/1‑140 (MQ=255)
                                                                        tGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAg                                                                 >  1:1044144/1‑140 (MQ=255)
                                                                         gtagtaTTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGt                                                                >  2:1374120/1‑140 (MQ=255)
                                                                         gtagtaTTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGt                                                                <  2:10907/140‑1 (MQ=255)
                                                                         gtagtaTTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGt                                                                <  1:1415650/140‑1 (MQ=255)
                                                                           agtaTTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGc                                                              >  1:1476536/1‑140 (MQ=255)
                                                                            gtaTTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGcc                                                             <  2:1321060/140‑1 (MQ=255)
                                                                            gtaTTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGcc                                                             <  2:411651/140‑1 (MQ=255)
                                                                            gtaTTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGcc                                                             <  2:693780/140‑1 (MQ=255)
                                                                            gtaTTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGcc                                                             <  1:363767/140‑1 (MQ=255)
                                                                            gtaTTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGcc                                                             <  1:243667/140‑1 (MQ=255)
                                                                            gtaTTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGcc                                                             <  2:414057/140‑1 (MQ=255)
                                                                                tCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCa                                                                  <  1:189139/131‑1 (MQ=255)
                                                                                tCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCa                                                                  >  2:189139/1‑131 (MQ=255)
                                                                                tCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGcc                                                             >  2:1686970/1‑136 (MQ=255)
                                                                                tCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGcc                                                             <  1:1686970/136‑1 (MQ=255)
                                                                                 cGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCa                                                                  >  1:1259943/1‑130 (MQ=255)
                                                                                 cGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCa                                                                  <  2:1259943/130‑1 (MQ=255)
                                                                                 cGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGcccc                                                           >  2:1591710/1‑137 (MQ=255)
                                                                                 cGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGcccc                                                           <  1:1591710/137‑1 (MQ=255)
                                                                                 cGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAg                                                        <  2:138967/140‑1 (MQ=255)
                                                                                 cGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAg                                                        >  2:1277888/1‑140 (MQ=255)
                                                                                  gTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAATCCGCCTGCGGCTTTCAGTGCCCCCAGt                                                       >  2:438155/1‑140 (MQ=255)
                                                                                    cAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTAt                                                     >  1:1503503/1‑140 (MQ=255)
                                                                                       ggTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGcc                                                  >  1:1663323/1‑140 (MQ=255)
                                                                                         tCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCa                                                                  >  2:259661/1‑122 (MQ=255)
                                                                                         tCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCa                                                                  <  1:259661/122‑1 (MQ=255)
                                                                                         tCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAg                                                        >  1:696895/1‑132 (MQ=255)
                                                                                         tCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAg                                                        <  2:696895/132‑1 (MQ=255)
                                                                                            tGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGGATGCCAACgg                                             >  1:807229/1‑140 (MQ=255)
                                                                                               tCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCaa                                          <  2:1660629/140‑1 (MQ=255)
                                                                                                 gCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAgg                                        >  1:380993/1‑140 (MQ=255)
                                                                                                  cTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGc                                       >  2:990734/1‑140 (MQ=255)
                                                                                                   tGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGcc                                      >  2:1276628/1‑140 (MQ=255)
                                                                                                   tGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGcc                                      <  1:990734/140‑1 (MQ=255)
                                                                                                    gAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCa                                     <  2:145599/140‑1 (MQ=255)
                                                                                                    gAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCa                                     <  2:1699190/140‑1 (MQ=255)
                                                                                                                ggCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTggcg                         <  1:206369/140‑1 (MQ=255)
                                                                                                                 gtccACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTggcgg                        >  2:830914/3‑140 (MQ=255)
                                                                                                                 gCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTggcgg                        >  1:1200219/1‑140 (MQ=255)
                                                                                                                 gCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTggcgg                        >  1:1480577/1‑140 (MQ=255)
                                                                                                                 gCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTggcgg                        >  1:581970/1‑140 (MQ=255)
                                                                                                                 gCCCACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTggcgg                        >  1:1106567/1‑140 (MQ=255)
                                                                                                                    cACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGt                     >  1:739034/1‑140 (MQ=255)
                                                                                                                    cACGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGt                     <  1:1270049/140‑1 (MQ=255)
                                                                                                                      cGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGc                   >  1:846407/1‑140 (MQ=255)
                                                                                                                      cGTAATGTTACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGc                   >  2:1330512/1‑140 (MQ=255)
                                                                                                                             ttACCTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCtg            <  1:1330512/140‑1 (MQ=255)
                                                                                                                                ccTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTg                    <  1:500181/129‑1 (MQ=255)
                                                                                                                                ccTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTg                    >  2:500181/1‑129 (MQ=255)
                                                                                                                                ccTTTTTTCACC‑TTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCtgg           >  2:167739/1‑137 (MQ=255)
                                                                                                                                ccTTTTTTCACC‑TTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCtgg           <  1:167739/137‑1 (MQ=255)
                                                                                                                                 cTTTTTTCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCtggtgg        >  1:494725/1‑140 (MQ=255)
                                                                                                                                  ttttttCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTggtggt       <  2:1480577/140‑1 (MQ=255)
                                                                                                                                     tttCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCa    >  2:970325/1‑140 (MQ=255)
                                                                                                                                     tttCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCa    <  2:1476536/140‑1 (MQ=255)
                                                                                                                                     tttCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCa    >  1:659281/1‑140 (MQ=255)
                                                                                                                                      ttCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCaa   <  2:380993/140‑1 (MQ=255)
                                                                                                                                       tCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAAt  >  1:903318/1‑140 (MQ=255)
                                                                                                                                       tCACCTTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGACAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAAt  <  2:934738/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                      
CAGGACGCTGAAATTGCACCAGATCGAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACC‑TTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAAT  >  NC_000913/1981309‑1981582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A