Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	183	98.6	3427438	96.9%	3321187	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,245,037	T→C	D578G (GAC→GGC)	cirA ←	ferric dihyroxybenzoylserine outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,245,037	0	T	C	100.0%	327.4 / NA	87	D578G (GAC→GGC)	cirA	ferric dihyroxybenzoylserine outer membrane transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (43/44); total (43/44)

CCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGTCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGACAGTTTCCATTCATCGTTGAACGGAATTTTCAGTTCG  >  NC_000913/2244903‑2245171

ccTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTccagcccagc                                                                                                                                   <  2:330462/140‑1 (MQ=255)

ccTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTccagcccagc                                                                                                                                   <  1:203880/140‑1 (MQ=255)

ccTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTccagcccagc                                                                                                                                   >  2:1656809/1‑140 (MQ=255)

 cTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCg                                                                                                                                  >  2:217378/1‑140 (MQ=255)

 cTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCg                                                                                                                                  >  2:726156/1‑140 (MQ=255)

 cTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCg                                                                                                                                  >  1:1349410/1‑140 (MQ=255)

  tGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGt                                                                                                                                 <  2:24069/140‑1 (MQ=255)

  tGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGt                                                                                                                                 <  1:217378/140‑1 (MQ=255)

  tGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGt                                                                                                                                 <  1:1593956/140‑1 (MQ=255)

  tGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGt                                                                                                                                 <  1:817714/140‑1 (MQ=255)

    ccAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTAc                                                                                                                               >  1:1112898/1‑140 (MQ=255)

       ggCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGt                                                                                                                            <  2:1112134/140‑1 (MQ=255)

        gCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGt                                                                                                                                 <  1:880367/134‑1 (MQ=255)

        gCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGt                                                                                                                                 >  2:880367/1‑134 (MQ=255)

        gCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGtt                                                                                                                           >  1:813593/1‑140 (MQ=255)

        gCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGtt                                                                                                                           <  1:319419/140‑1 (MQ=255)

        gCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGtt                                                                                                                           <  1:125207/140‑1 (MQ=255)

          cgcgCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAg                                                                                                                         <  1:555441/140‑1 (MQ=255)

          cgcgCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAg                                                                                                                         <  1:1656809/140‑1 (MQ=255)

           gcgcCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTccagcccagc                                                                                                                                   <  1:964063/129‑1 (MQ=255)

           gcgcCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTccagcccagc                                                                                                                                   >  2:964063/1‑129 (MQ=255)

            cgcCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCa                                                                                                                       >  2:1319152/1‑140 (MQ=255)

             gcCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAg                                                                                                                      <  1:1386937/140‑1 (MQ=255)

             gcCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAg                                                                                                                      <  1:1678230/140‑1 (MQ=255)

               cGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTa                                                                                                                    <  2:1420423/140‑1 (MQ=255)

                  tATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATgg                                                                                                                 >  1:873596/1‑140 (MQ=255)

                      ccAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACg                                                                                                             <  2:180652/140‑1 (MQ=255)

                        aGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGc                                                                                                           <  2:567124/140‑1 (MQ=255)

                            ggTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGAt                                                                                                       >  1:566296/1‑140 (MQ=255)

                               ataACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCgg                                                                                                    <  2:1540413/140‑1 (MQ=255)

                               ataACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCgg                                                                                                    >  1:494965/1‑140 (MQ=255)

                                taACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCC‑GTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACg                                                                                                             >  2:1565365/1‑129 (MQ=255)

                                taACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCC‑GTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACg                                                                                                             <  1:1565365/129‑1 (MQ=255)

                                      cccGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCgg                                                                                             >  2:717423/1‑140 (MQ=255)

                                        cGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCTCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGgtt                                                                                           >  1:244061/1‑140 (MQ=255)

                                        cGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGgtt                                                                                           >  2:1630278/1‑140 (MQ=255)

                                        cGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGgtt                                                                                           >  1:783709/1‑140 (MQ=255)

                                          gtgtTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGgtttg                                                                                         >  2:808145/1‑140 (MQ=255)

                                                     gTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGtt                                                                              >  2:1000795/1‑140 (MQ=255)

                                                       cgcgCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGc                                                                            >  2:1559286/1‑140 (MQ=255)

                                                        gcgcTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCt                                                                           <  2:174955/140‑1 (MQ=255)

                                                        gcgcTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCt                                                                           >  2:1314343/1‑140 (MQ=255)

                                                           cTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGAc                                                                        <  2:1112898/140‑1 (MQ=255)

                                                                ggCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCAc                                                                   >  2:420453/1‑140 (MQ=255)

                                                                  cgcgTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGa                                                                 <  1:675062/140‑1 (MQ=255)

                                                                  cgcgTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGa                                                                 <  1:54429/140‑1 (MQ=255)

                                                                    cgTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGAcc                                                               <  2:494965/140‑1 (MQ=255)

                                                                    cgTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGAcc                                                               <  2:854527/140‑1 (MQ=255)

                                                                    cgTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGAcc                                                               <  1:332107/140‑1 (MQ=255)

                                                                    cgTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGAcc                                                               <  2:1349410/140‑1 (MQ=255)

                                                                     gTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCa                                                              <  1:1314343/140‑1 (MQ=255)

                                                                      tttCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCAt                                                             <  2:813593/140‑1 (MQ=255)

                                                                      tttCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCAt                                                             <  1:1559286/140‑1 (MQ=255)

                                                                      tttCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCAt                                                             >  2:1390217/1‑140 (MQ=255)

                                                                            cccGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGt                                                       <  1:1630278/140‑1 (MQ=255)

                                                                            cccGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGt                                                       <  2:105414/140‑1 (MQ=255)

                                                                              cGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAg                                                     <  1:1416524/140‑1 (MQ=255)

                                                                              cGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAg                                                     >  1:6179/1‑140 (MQ=255)

                                                                              cGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAg                                                     <  2:873596/140‑1 (MQ=255)

                                                                                 tataGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCAt                                                             <  2:514835/129‑1 (MQ=255)

                                                                                 tataGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCAt                                                             >  1:514835/1‑129 (MQ=255)

                                                                                      tGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGtt                                             >  2:515398/1‑140 (MQ=255)

                                                                                           aGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGAt                                          <  2:782882/138‑1 (MQ=255)

                                                                                           aGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGAt                                          >  1:782882/1‑138 (MQ=255)

                                                                                           aGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCg                                        >  1:675555/1‑140 (MQ=255)

                                                                                           aGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCg                                        >  2:1294795/1‑140 (MQ=255)

                                                                                           aGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCg                                        >  2:1121789/1‑140 (MQ=255)

                                                                                           aGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCg                                        >  1:204310/1‑140 (MQ=255)

                                                                                            gAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGa                                       <  2:783709/140‑1 (MQ=255)

                                                                                            gAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGa                                       <  2:197558/140‑1 (MQ=255)

                                                                                            gAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGa                                       <  1:1294795/140‑1 (MQ=255)

                                                                                             aaaCATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGAc                                      <  1:1056364/140‑1 (MQ=255)

                                                                                                cATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGACAGt                                   <  1:1319152/140‑1 (MQ=255)

                                                                                                cATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGACAGt                                   >  1:175695/1‑140 (MQ=255)

                                                                                                cATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGACAGt                                   >  1:372723/1‑140 (MQ=255)

                                                                                                       tGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGAc                                      <  2:594004/130‑1 (MQ=255)

                                                                                                       tGACCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGAc                                      >  1:594004/1‑130 (MQ=255)

                                                                                                         aCCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGACAGTTTCCATTCa                          >  2:1349745/1‑140 (MQ=255)

                                                                                                         aCCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGACAGTTTCCATTCa                          >  2:854513/1‑140 (MQ=255)

                                                                                                         aCCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGACAGTTTCCATTCa                          >  2:70912/1‑140 (MQ=255)

                                                                                                         aCCAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGACAGTTTCCATTCa                          >  2:759735/1‑140 (MQ=255)

                                                                                                          ccAGTCTTCCAGCGCCAGTGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGACAGTTTcc                               <  1:102978/134‑1 (MQ=255)

                                                                                                          ccAGTCTTCCAGCGCCAGTGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGACAGTTTcc                               >  2:102978/1‑134 (MQ=255)

                                                                                                          ccAGTCTTCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGACAGTTTCCATTCAt                         >  2:48974/1‑140 (MQ=255)

                                                                                                                ttCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGACAGTTTCCATTCATCGTTGa                   <  1:726156/140‑1 (MQ=255)

                                                                                                                ttCCAGCGCCAGCGGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGACAGTTTCCATTCATCGTTGa                   <  2:1209420/140‑1 (MQ=255)

                                                                                                                        ccagcgGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGACAGTTTCCATTCATCGTTGAACGGAAtt           >  1:1391847/1‑140 (MQ=255)

                                                                                                                        ccagcgGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGACAGTTTCCATTCATCGTTGAACGGAAtt           >  1:1378008/1‑140 (MQ=255)

                                                                                                                        ccagcgGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGACAGTTTCCATTCATCGTTGAACGGAAtt           >  2:970024/1‑140 (MQ=255)

                                                                                                                          agcgGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGACAGTTTCCATTCATCGTTGAACGGAAtttt         >  1:29850/1‑140 (MQ=255)

                                                                                                                           gcgGTTTCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTATGTGTAGTTGATCGACAGTTTCCATTCATCGTTGAACGGAATTTTc        >  1:1642933/1‑140 (MQ=255)

                                                                                                                               tttCCAGCCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGACAGTTTCCATTCATCGTTGAACGGAATTTTCAGtt    <  2:204310/140‑1 (MQ=255)

                                                                                                                                 tccagcccagCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGACAGTTTCCATTCATCGTTGAACGGAATTTTCAGTTCg  >  1:460940/1‑140 (MQ=255)

CCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATAGTGCCCAGAAACATAGAATGACCAGTCTTCCAGCGCCAGCGGTTTCCAGTCCAGCGTACCGTTAGCAGTATGGAACGGCAGATCGGATAGCGGTTTGTTTTCGCCGTTGCTGACATCACGACCATCGTTGTAGGTGTAGTTGATCGACAGTTTCCATTCATCGTTGAACGGAATTTTCAGTTCG  >  NC_000913/2244903‑2245171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A