Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	183	98.6	3427438	96.9%	3321187	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,783	T→C	intergenic (‑15/+80)	pyrE ← / ← rph	orotate phosphoribosyltransferase/truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,783	0	T	C	100.0%	195.5 / NA	53	intergenic (‑15/+80)	pyrE/rph	orotate phosphoribosyltransferase/truncated RNase PH
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/26); total (27/26)

CCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑C‑CC‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCA  >  NC_000913/3815655‑3815917

cccGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCt                                                                                                                                >  1:1062845/1‑140 (MQ=255)

cccGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCt                                                                                                                                >  2:330011/1‑140 (MQ=255)

cccGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCt                                                                                                                                >  2:1275140/1‑140 (MQ=255)

  cGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTAc                                                                                                                              >  1:923402/1‑140 (MQ=255)

   ggCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACa                                                                                                                             <  2:753939/140‑1 (MQ=255)

      gTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGAc                                                                                                                          <  1:58459/140‑1 (MQ=255)

              aaTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACaaaaaaaa                                                                                                                  <  2:794576/140‑1 (MQ=255)

                 aGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGc                                                                                                               >  2:216670/1‑140 (MQ=255)

                  ggggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCg                                                                                                              <  2:1533481/140‑1 (MQ=255)

                    ggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGAc                                                                                                            <  2:1710449/140‑1 (MQ=255)

                       ttttGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACtca                                                                                                         >  1:1451325/1‑140 (MQ=255)

                       ttttGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACtca                                                                                                         >  1:619467/1‑140 (MQ=255)

                        tttGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACtcat                                                                                                        >  2:145955/1‑140 (MQ=255)

                        tttGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACtcat                                                                                                        >  2:492866/1‑140 (MQ=255)

                         ttGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACtcatc                                                                                                       >  1:752828/1‑140 (MQ=255)

                              cccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCg                                                                                                  <  1:330011/140‑1 (MQ=255)

                                                cTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGc                                                                                <  1:492866/140‑1 (MQ=255)

                                                    ccAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgc                                                                            >  1:1648242/1‑140 (MQ=255)

                                                     cAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgcg                                                                           <  2:1062845/140‑1 (MQ=255)

                                                       aaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgcc                                                                         <  2:1451325/140‑1 (MQ=255)

                                                         cTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGATCCTCACCTTACTTTTCTACAGACAAAAAAAATGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgccgc                                                                       >  2:1591463/1‑140 (MQ=255)

                                                                  cTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                              <  2:619467/140‑1 (MQ=255)

                                                                  cTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                              >  1:891369/1‑140 (MQ=255)

                                                                    gcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCg                                                            >  1:1327592/1‑140 (MQ=255)

                                                                    gcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCg                                                            >  2:1101058/1‑140 (MQ=255)

                                                                    gcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCg                                                            >  1:478295/1‑140 (MQ=255)

                                                                     cttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGc                                                           <  1:1627893/140‑1 (MQ=255)

                                                                            aGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACa                                                       >  2:659176/1‑137 (MQ=255)

                                                                            aGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACa                                                       <  1:659176/137‑1 (MQ=255)

                                                                            aGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATg                                                    >  1:653520/1‑140 (MQ=255)

                                                                              cgcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGga                                                  >  2:723181/1‑140 (MQ=255)

                                                                               gcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgat                                                 >  1:1702472/1‑140 (MQ=255)

                                                                                cAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgatt                                                <  2:1327592/140‑1 (MQ=255)

                                                                                      cAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgatt‑c                                         <  2:752828/140‑1 (MQ=255)

                                                                                           aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑c‑cc‑cct                                  <  1:511391/140‑3 (MQ=255)

                                                                                           aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑c‑cc‑cct                                  <  2:1648242/140‑3 (MQ=255)

                                                                                               ggCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑CTCggg                               >  2:1432268/1‑140 (MQ=255)

                                                                                                gcgcTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑CTCGGGc                              <  2:891369/140‑1 (MQ=255)

                                                                                                 cgcTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑CTCGGGcc                             >  2:759480/1‑140 (MQ=255)

                                                                                                   cTGATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑CTCGGGCCag                           <  2:1702472/140‑1 (MQ=255)

                                                                                                     gATATGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑CTCGGGCCagag                         <  2:137074/140‑1 (MQ=255)

                                                                                                      atatGGTTTCACGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCATTCTGCGTCGCTACAATGGATTCGATT‑C‑CCACTCGGGCCAGAGc                        >  2:1250630/1‑140 (MQ=255)

                                                                                                        atGGTTTCATGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑CTCGGGCCAGAGCca                      <  1:1101058/140‑1 (MQ=255)

                                                                                                                aTGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑CTCGGGCCAGAGCCAACAAGATg              >  2:1085809/1‑140 (MQ=255)

                                                                                                                aTGCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑CTCGGGCCAGAGCCAACAAGATg              >  1:718855/1‑140 (MQ=255)

                                                                                                                  gCCTTCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑CTCGGGCCAGAGCCAACAAGATGAg            >  2:1165820/1‑140 (MQ=255)

                                                                                                                     ttCGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑CACC‑CTCGGGCCAGAGCCAACAAGATGAGTAg         <  1:1591463/140‑1 (MQ=255)

                                                                                                                       cGCTCCTCACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑CTCGGGCCAGAGCCAACAAGATGAGTAGct       >  2:1432800/1‑140 (MQ=255)

                                                                                                                          tcctcACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt    <  1:1165820/140‑1 (MQ=255)

                                                                                                                          tcctcACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt    <  2:923402/140‑1 (MQ=255)

                                                                                                                          tcctcACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCACCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt    <  1:145955/140‑1 (MQ=255)

                                                                                                                            ctcACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCa  <  1:723181/140‑1 (MQ=255)

                                                                                                                            ctcACCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCa  <  1:1275140/140‑1 (MQ=255)

CCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑C‑CC‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCA  >  NC_000913/3815655‑3815917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A