Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 183 98.6 3427438 96.9% 3321187 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,397,392 T→C L443P (CTG→CCG)  amiB → N‑acetylmuramoyl‑L‑alanine amidase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,397,3920TC98.0% 176.9 / ‑5.8 50L443P (CTG→CCG) amiBN‑acetylmuramoyl‑L‑alanine amidase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (41/8);  minor base A (1/0);  total (42/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCG  >  NC_000913/4397255‑4397528
                                                                                                                                         |                                                                                                                                        
cgacgaTTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCAcgccgc                                                                                                                                        <  1:419641/140‑1 (MQ=255)
cgacgaTTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCAcgccgc                                                                                                                                        <  1:1535966/140‑1 (MQ=255)
   cgaTTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCaa                                                                                                                                     >  1:113824/1‑140 (MQ=255)
           aacaacAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGa                                                                                                                             <  2:113824/140‑1 (MQ=255)
             caacaGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACg                                                                                                                           >  1:1600325/1‑140 (MQ=255)
              aacaGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGa                                                                                                                          <  2:910520/140‑1 (MQ=255)
                caGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGAtt                                                                                                                        >  1:1078575/1‑140 (MQ=255)
                caGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGAtt                                                                                                                        >  1:670611/1‑140 (MQ=255)
                   cTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGAt                                                                                                                     >  1:989891/1‑140 (MQ=255)
                     ggCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGc                                                                                                                   <  1:272862/140‑1 (MQ=255)
                               aTTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGt                                                                                                         <  2:168956/140‑1 (MQ=255)
                                tttACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTc                                                                                                        >  1:678173/1‑140 (MQ=255)
                                       aGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGc                                                                                                 >  2:1073962/1‑140 (MQ=255)
                                         gCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCca                                                                                               >  1:1340328/1‑140 (MQ=255)
                                         gCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCca                                                                                               >  1:60344/1‑140 (MQ=255)
                                          ccTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCcac                                                                                              >  2:1494195/1‑140 (MQ=255)
                                           cTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCcaca                                                                                             >  1:1443070/1‑140 (MQ=255)
                                            tGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACaa                                                                                            >  2:847000/1‑140 (MQ=255)
                                              cgcAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACt                                                                                          >  1:1174848/1‑140 (MQ=255)
                                                aaaTTACTCCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCAAAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACAAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTgg                                                                                        <  2:767568/139‑1 (MQ=255)
                                                   ttACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGa                                                                                     >  1:1293610/1‑140 (MQ=255)
                                                   ttACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGa                                                                                     >  1:1258826/1‑140 (MQ=255)
                                                        cccTTGCGCATCCGATGCAATCTGCGCCGCGGGGTGCAACGGCACAACCTGCCAGTACGGTGACGACGCCAGATCGCCCGCCGCCAAACTAAGGACGATTGATGCAAATTCAGGTCTTACCGCCACAACTGGCGAACCAg                                                                                <  2:1140652/139‑1 (MQ=255)
                                                          ctggcgcACCGGATGCAATCGGCGCCGCGGGGTGCAAGGGCACAACCTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTTTTACCGCCACAACTGGCGAACCAGAt                                                                              <  2:60344/137‑1 (MQ=255)
                                                          cTTGCGCATCCGATGCAATCTGCGCCGCGGGGTGCAACGGCACAACCTGCCAGTAGGGTGACGACGCCAGATCGCCCGCCGCCAAACAAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGAt                                                                              <  2:1092060/140‑1 (MQ=255)
                                                             gcgcATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGc                                                                           >  1:313566/1‑140 (MQ=255)
                                                             gcgcATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGc                                                                           >  2:1348804/1‑140 (MQ=255)
                                                             gcgcATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGc                                                                           >  1:1416189/1‑140 (MQ=255)
                                                             gcgcATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGc                                                                           >  1:789140/1‑140 (MQ=255)
                                                             gcgcATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGc                                                                           >  1:1169720/1‑140 (MQ=255)
                                                             gcgcACCCGATGAAATCTGCGCCGCGGGGTGCACCGGCAAAACCTCCCAGTAGGGTGACGACGCCAGATCGCCCGCCGCAAAACTAAGGACGTTTGAGCCCAATTCAGGTTTTACCGCCACAACTGGCGAACCAGATTGc                                                                           <  2:1682229/140‑1 (MQ=255)
                                                              cgcATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGcc                                                                          >  2:886247/1‑140 (MQ=255)
                                                               gcATCCGATGCAATCTCCGCCGCAGGGGGCAACGGCACAACCTGCCAGTGCGGTGACGCCCCCAGATCGCACGCCCCCAAACAAAGGACGATTGATGCCATTTCGGGTCTTACCGCCACAACTGGCGAACCAGATTGCCg                                                                         <  2:880393/140‑1 (MQ=255)
                                                                cctCCGATCCAATCGGCGCCGGGGGGGGCAAGGGCAAAAACGGCGAGGAGGGCGACGCCGCCAGATCGCCCGCCGCCAAACAAGGGACGATTAATGCAAATTCGGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGc                                                                        <  2:421241/138‑1 (MQ=255)
                                                                 aTCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCa                                                                       >  1:1004256/1‑140 (MQ=255)
                                                                  cccGATGCAATCGGCGCCGCAGGGTGCACCGGCAAAACCTGCCAGTACGGTGACGACGCCAGATCGCCCGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCag                                                                      <  2:989891/139‑1 (MQ=255)
                                                                       tGCGATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCGCAACTGGCGAACCAGATTGCCGCaggtgag                                                                 <  1:1294057/140‑1 (MQ=255)
                                                                            tCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAggtggt                                                            >  2:1640704/1‑140 (MQ=255)
                                                                                    gcAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGCGAGGTGGTCGAGCGAc                                                    >  1:738300/1‑140 (MQ=255)
                                                                                     cAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTGAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGAcc                                                   >  2:1496555/1‑140 (MQ=255)
                                                                                     cAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGAcc                                                   >  1:348489/1‑140 (MQ=255)
                                                                                          tGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGCGAGGTGGTCGAGCGACCTGCGt                                              >  1:1489316/1‑140 (MQ=255)
                                                                                             aaCGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCgg                                           <  2:348489/140‑1 (MQ=255)
                                                                                             aaCGGCAAAACCTGCCAGTACGGTGACGACGCCAGACCGCCCGCCGCCAAACTAAGGACGATTGATGCAAATTCACGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCgg                                           <  2:1169720/140‑1 (MQ=255)
                                                                                              gcGGCAAAAACTGCCAGTACGGGGACGACGCCAGACCGCACGACGCAAAACTAAGGACGATTGATGCAAATTCGGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGt                                          <  2:1174848/139‑1 (MQ=255)
                                                                                               cGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCAAAACTAAGGACGTTTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTa                                         <  2:1363008/140‑1 (MQ=255)
                                                                                                 gCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGt                                       <  2:691719/140‑1 (MQ=255)
                                                                                                  cacaAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTc                                      >  2:35964/1‑140 (MQ=255)
                                                                                                  cacaAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTc                                      >  2:1507867/1‑140 (MQ=255)
                                                                                                  cacaAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTc                                      >  2:231578/1‑140 (MQ=255)
                                                                                                    caAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCg                                            <  1:1512380/132‑1 (MQ=255)
                                                                                                    caAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCaa                                    >  2:1123360/1‑140 (MQ=255)
                                                                                                    caAACTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCaa                                    <  2:1004256/140‑1 (MQ=255)
                                                                                                    caAACTGCCAGTACGGTGACGACGCCAGATCGCACGCAGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCg                                            >  2:1512380/1‑132 (MQ=255)
                                                                                                      aaCTGCCAGTACGGTGCCGCCGCCAGATCGCCCGCCGCCAAACAAAGGAGGATTGAGGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAg                                  <  2:447444/140‑1 (MQ=255)
                                                                                                      aaCTGCCAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAg                                  <  1:231578/140‑1 (MQ=255)
                                                                                                           ccAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTa                             >  2:1216617/1‑140 (MQ=255)
                                                                                                           ccAGTACGGTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTa                             >  2:970153/1‑140 (MQ=255)
                                                                                                                   gTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGaaaa                     >  1:1660317/1‑140 (MQ=255)
                                                                                                                   gTGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATGGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGaaaa                     >  2:451300/1‑140 (MQ=255)
                                                                                                                    tGACGCCGCCAGACCGCCCGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAAc                    <  2:1600325/140‑1 (MQ=255)
                                                                                                                    tGACGACGCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAAc                    >  2:189533/1‑140 (MQ=255)
                                                                                                                         acgCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGGCt               >  2:1049815/1‑140 (MQ=255)
                                                                                                                         acgCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCt               >  2:1550495/1‑140 (MQ=255)
                                                                                                                         acgCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCt               <  2:1340328/140‑1 (MQ=255)
                                                                                                                         acgCCAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCt               >  1:1646165/1‑140 (MQ=255)
                                                                                                                             cAGATCGCACGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGAt           >  2:1352236/1‑140 (MQ=255)
                                                                                                                                   gCACCCCGCAAAACTAAGGACGATTGATGCCAATTCAGTTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGt     <  1:1073962/140‑1 (MQ=255)
                                                                                                                                     aCGCCGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGc   >  2:493890/1‑140 (MQ=255)
                                                                                                                                      cgccgcCAACCTAAGGGCGATTGATGCCAATACAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCg  <  1:273886/140‑1 (MQ=255)
                                                                                                                                      cgccgcCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCg  <  1:1550495/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCG  >  NC_000913/4397255‑4397528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A