Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	210,131	+G	coding (453/2142 nt)	ldcC →	lysine decarboxylase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	210,127	1	.	G	98.5%	487.5 / ‑1.0	136	coding (449/2142 nt)	ldcC	lysine decarboxylase 2
Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (73/61); total (74/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01

Only 100 of 148 total aligned reads displayed.

GAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCC‑GGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCACCGAGCTTGGTTCGTTGCTCGACCACACC  >  NC_000913/209988‑210260

gAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGcc                                                                                                                                        <  1:355627/140‑1 (MQ=255)

   tatGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCC‑ggg                                                                                                                                    <  2:1221877/140‑1 (MQ=255)

   tatGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCC‑ggg                                                                                                                                    >  2:253803/1‑140 (MQ=255)

   tatGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCC‑ggg                                                                                                                                    >  2:751258/1‑140 (MQ=255)

    atGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCC‑gggg                                                                                                                                   >  2:34825/1‑140 (MQ=255)

        gcTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCC‑gggggcat                                                                                                                               <  1:1679198/140‑5 (MQ=255)

        gcTGGGACAGGAGGAAGATATGGCAATTCGATTGCGTCAGTACACCGACAAATATCTTGATAAAATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCC‑gggggcat                                                                                                                               <  2:320466/140‑5 (MQ=255)

           ggggCAGGCGGAAGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCC‑ggg                                                                                                                                    >  2:2403246/1‑132 (MQ=255)

           ggggCAGGCGGAAGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCC‑ggg                                                                                                                                    <  1:2403246/132‑1 (MQ=255)

            gggCAGGCGGAAGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATgg                                                                                                                            >  2:1062497/1‑140 (MQ=255)

             ggCAGGCGGAAGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATggg                                                                                                                           <  2:1464975/140‑1 (MQ=255)

             ggCAGGCGGAAGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATggg                                                                                                                           <  2:783907/140‑1 (MQ=255)

                     gAAGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCg                                                                                                                   <  1:1114484/140‑1 (MQ=255)

                     gAAGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCg                                                                                                                   <  2:2068027/140‑1 (MQ=255)

                      aaGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGc                                                                                                                  >  1:872478/1‑140 (MQ=255)

                             cGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCaa                                                                                                           >  1:1495463/1‑140 (MQ=255)

                                cATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCaaaaa                                                                                                        >  2:1884417/1‑140 (MQ=255)

                                 aTTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCaaaaaa                                                                                                       <  1:1863451/140‑1 (MQ=255)

                                   tCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGc                                                                                                     >  2:862009/1‑140 (MQ=255)

                                    cGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGcc                                                                                                    >  2:2362605/1‑140 (MQ=255)

                                    cGTATGCGTCAGTACACCGACGAATATCTCGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGcc                                                                                                    >  1:767865/1‑140 (MQ=255)

                                           gTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTgg                                                                                             <  1:2226148/140‑1 (MQ=255)

                                               gTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGctgt                                                                                         <  1:2174527/140‑1 (MQ=255)

                                                     cgacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGttt                                                                                   >  2:863202/1‑140 (MQ=255)

                                                     cgacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGttt                                                                                   >  2:1835678/1‑140 (MQ=255)

                                                     cgacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGttt                                                                                   >  1:722653/1‑140 (MQ=255)

                                                     cgacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGttt                                                                                   >  1:374268/1‑140 (MQ=255)

                                                     cgacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGttt                                                                                   >  2:793819/1‑140 (MQ=255)

                                                     cgacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGttt                                                                                   >  2:1259433/1‑140 (MQ=255)

                                                      gacgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGtttt                                                                                  >  1:1768614/1‑140 (MQ=255)

                                                       acgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTa                                                                                 >  2:652002/1‑140 (MQ=255)

                                                       acgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTa                                                                                 >  1:2267979/1‑140 (MQ=255)

                                                       acgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTa                                                                                 >  1:506473/1‑140 (MQ=255)

                                                       acgaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTa                                                                                 >  1:2187905/1‑140 (MQ=255)

                                                         gaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTAt                                                                                >  2:1063067/1‑139 (MQ=255)

                                                         gaATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTAt                                                                                <  1:1063067/139‑1 (MQ=255)

                                                          aaTATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGa                                                                              >  1:487192/1‑140 (MQ=255)

                                                                  gATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTcg                                                                      <  2:2187905/140‑1 (MQ=255)

                                                                  gATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTcg                                                                      >  1:2401461/1‑140 (MQ=255)

                                                                   aTAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTcgg                                                                     <  2:2267979/140‑1 (MQ=255)

                                                                    tAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTcggc                                                                    <  1:1835678/140‑1 (MQ=255)

                                                                    tAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTcggc                                                                    <  2:2186430/140‑1 (MQ=255)

                                                                    aaaCATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTcggc                                                                    <  2:2303282/139‑1 (MQ=255)

                                                                      aCATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTcggcgg                                                                  <  1:755969/140‑1 (MQ=255)

                                                                       cATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCGCCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCggg                                                                 >  2:2098024/1‑140 (MQ=255)

                                                                       cATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCggg                                                                 >  1:1738555/1‑140 (MQ=255)

                                                                        aTTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGa                                                                >  1:2066423/1‑140 (MQ=255)

                                                                          tACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAAt                                                              <  1:1259433/140‑1 (MQ=255)

                                                                          tACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAAt                                                              >  2:762545/1‑140 (MQ=255)

                                                                          tACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAAt                                                              <  1:2326141/140‑1 (MQ=255)

                                                                            cacCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATAc                                                            >  2:1650327/1‑140 (MQ=255)

                                                                            cacCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATAc                                                            >  2:895429/1‑140 (MQ=255)

                                                                              ccgccgTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATActc                                                          >  2:1919278/1‑140 (MQ=255)

                                                                                gccgTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCtt                                                        <  2:872478/140‑1 (MQ=255)

                                                                                 ccgTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTcgg                                                                     >  1:1503393/1‑126 (MQ=255)

                                                                                 ccgTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTa                                                       >  1:171074/1‑140 (MQ=255)

                                                                                 ccgTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTa                                                       >  2:1641876/1‑140 (MQ=255)

                                                                                  cgTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTaa                                                      >  2:1299985/1‑140 (MQ=255)

                                                                                    ttCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGaa                                                               >  1:1586849/1‑129 (MQ=255)

                                                                                    ttCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAgg                                                    >  1:709513/1‑140 (MQ=255)

                                                                                    ttCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAgg                                                    >  1:616489/1‑140 (MQ=255)

                                                                                      cACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCt                                                  <  1:751258/140‑1 (MQ=255)

                                                                                      cACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCt                                                  <  2:2140963/140‑1 (MQ=255)

                                                                                      cACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCt                                                  <  2:374268/140‑1 (MQ=255)

                                                                                        cGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGa                                                <  1:462271/140‑1 (MQ=255)

                                                                                         gAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGAt                                               <  2:2400785/140‑1 (MQ=255)

                                                                                         gAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGAt                                               <  1:1299985/140‑1 (MQ=255)

                                                                                         gAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGAt                                               <  2:1441421/140‑1 (MQ=255)

                                                                                             gCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGGTTTATGATTTTTTCGGCGGGAATACTCTTAAGGGTGATGtgt                                           >  2:2245648/1‑138 (MQ=255)

                                                                                                   tttACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTAttt                                     >  1:2364597/1‑140 (MQ=255)

                                                                                                     tACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCg                                   <  2:1742950/140‑1 (MQ=255)

                                                                                                       ccTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCt                                                  <  2:692480/123‑1 (MQ=255)

                                                                                                       ccTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCt                                                  >  1:692480/1‑123 (MQ=255)

                                                                                                        cTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTc                                <  1:1155719/140‑1 (MQ=255)

                                                                                                         tACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCa                               <  2:677449/140‑1 (MQ=255)

                                                                                                         tACGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCa                               <  2:1495463/140‑1 (MQ=255)

                                                                                                          aCGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCAc                              <  1:559737/140‑1 (MQ=255)

                                                                                                           cGTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCAcc                             <  2:1494415/140‑1 (MQ=255)

                                                                                                            gTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCACCg                            >  2:1538363/1‑140 (MQ=255)

                                                                                                            gTCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGGCTCTTTTTCTGTCACCg                            >  2:1155719/1‑140 (MQ=255)

                                                                                                             tCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATGTGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCACCGa                           >  1:45515/1‑140 (MQ=255)

                                                                                                             tCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGCATAATCTTAAGGCTGCGGTTTCTTTTTGCGTCACCgc                           >  1:1636102/1‑139 (MQ=255)

                                                                                                             tCAAAGAGCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAAAACTCTTAAGGCTGATGTCTCTATTTCGGTCACCGa                           >  2:2022234/1‑140 (MQ=255)

                                                                                                             tCAAAGAGCGGAAGTACACCTTTTGTACGCC‑GGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCACCGAg                          >  1:1090586/1‑140 (MQ=255)

                                                                                                                 agagCGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCACCGAGCtt                       <  2:1821484/140‑1 (MQ=255)

                                                                                                                     cGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCACCGAGCTTGGtt                   >  2:157050/1‑140 (MQ=255)

                                                                                                                     cGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCACCGAGCTTGGtt                   >  1:869750/1‑140 (MQ=255)

                                                                                                                     cGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCACCGAGCTTGGtt                   >  1:2372131/1‑140 (MQ=255)

                                                                                                                     cGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCACCGAGCTTGGtt                   >  1:1342792/1‑140 (MQ=255)

                                                                                                                     cGGAAGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCACCGAGCTTGGtt                   >  1:109407/1‑140 (MQ=255)

                                                                                                                        aaGTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCACCGAGCTTGGTTCGt                <  2:1792471/140‑1 (MQ=255)

                                                                                                                          gTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCACCGAGCTTGGTTCGTTg              <  1:807032/140‑1 (MQ=255)

                                                                                                                          gTACACCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCACCGAGCTTGGTTCGTTg              <  1:680273/140‑1 (MQ=255)

                                                                                                                             cacCTTTTGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGGATAATCTTAAGGCTGATGTCTCTATTTTGGTCACCGAGCTTGGGTCGTTGCTc           >  2:1339279/1‑140 (MQ=255)

                                                                                                                                  tttGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCACCGAGCTTGGTTCGTTGCTCGACca      <  1:1919278/140‑1 (MQ=255)

                                                                                                                                  tttGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCACCGAGCTTGGTTCGTTGCTCGACca      >  1:1469393/1‑140 (MQ=255)

                                                                                                                                  tttGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCACCGAGCTTGGTTCGTTGCTCGACca      >  2:2326169/1‑140 (MQ=255)

                                                                                                                                   ttGTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCACCGAGCTTGGTTCGTTGCTCGACcac     >  2:55612/1‑140 (MQ=255)

                                                                                                                                     gTACGCCGGGGGCATGTGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCACCGAGCTTGGTTCGTTGCTCGACCacac   <  2:45515/140‑1 (MQ=255)

                                                                                                                                     gTACGCCGGGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCACCGAGCTTGGTTCGTTGCTCGACCacac   <  2:709513/140‑1 (MQ=255)

GAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGTCAGTACACCGACGAATATCTTGATAACATTACACCGCCGTTCACGAAAGCCTTGTTTACCTACGTCAAAGAGCGGAAGTACACCTTTTGTACGCC‑GGGGCATATGGGCGGCACCGCATATCAAAAAAGCCCGGTTGGCTGTCTGTTTTATGATTTTTTCGGCGGGAATACTCTTAAGGCTGATGTCTCTATTTCGGTCACCGAGCTTGGTTCGTTGCTCGACCACACC  >  NC_000913/209988‑210260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A