Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,001,935	+G	intergenic (‑146/+175)	fliA ← / ← fliC	RNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,001,931	1	.	G	98.7%	265.8 / ‑3.3	76	intergenic (‑142/+179)	fliA/fliC	RNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (45/30); total (45/31)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.71e-01

AACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGC‑‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCA  >  NC_000913/2001794‑2002063

aacgccccTGGTTTGTATGAGTTATCGGCATGATTATCCTTTTCGGCGGGTTTTTTAAGCGGACGTTTGGGGGGGGAAAGGGGGGTTTATTGGGGGG‑TTAAAGGTAAATCCAAGGAAAAAAAAAC‑CCCCGCCGGTGGC‑‑gg                                                                                                                                    <  2:588168/135‑1 (MQ=255)

  cAGCCCTGCTTTATATGATTTATCGGCATGATTATCCGTTTCTGCAGGTTTTTTAATCGGATGATTAGGGGGTGAAATGGGGGGTTATTGGGGGG‑TTACAGGAAAATTCCAGGAAAAAAAAAC‑CCCCGCCGGTGGC‑‑gggg                                                                                                                                  <  2:308761/140‑1 (MQ=255)

        tGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGATGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCa                                                                                                                             >  1:308761/1‑140 (MQ=255)

        tGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCa                                                                                                                             >  2:496998/1‑140 (MQ=255)

         gctttATATGATTTATCGGCATTATTATCCTTTTCTGGGGGTTTTTTAATCGGACGATTAGTGGGTAAAAGGGGGGTTTATTGGGGGG‑TTACAGGAAAATCCCAGGCAAAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCAc                                                                                                                            <  2:1407077/137‑1 (MQ=255)

          cGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACg                                                                                                                           >  1:1984262/1‑140 (MQ=255)

             tataTGATTTATCGGCATGATTATCCGTTTCTGCGGGTTTTTTAATCGGACGATTGGTGGGTAAAATGGGGGGTTTTTTGGGGG‑TTACAGGTAAATTCCAGGCAAAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTg                                                                                                                        <  2:294398/140‑1 (MQ=255)

                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACaa                                                                                                                 >  1:1256741/1‑140 (MQ=255)

                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACaa                                                                                                                 >  1:2142036/1‑140 (MQ=255)

                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACaa                                                                                                                 >  1:2280428/1‑140 (MQ=255)

                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACaa                                                                                                                 >  1:1851447/1‑140 (MQ=255)

                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACaa                                                                                                                 >  1:2255329/1‑140 (MQ=255)

                     ttATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACaaa                                                                                                                >  2:1366334/1‑140 (MQ=255)

                      tATGGGCATGATTATCCGTTTCTGCAGGTTTTTTAATCGGATGTTTTGGGGGTGAAAGGAGGGTTTATTTGGGGG‑TTAGAGGTAAATTCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAAt                                                                                                               <  2:1984262/140‑1 (MQ=255)

                      tATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAAt                                                                                                               >  2:246744/1‑140 (MQ=255)

                       ttCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAAGGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAC‑CCCCGCCGGTGGC‑‑GGGGAAGCACGTTGCTGACAAATTg                                                                                                             <  1:11543/139‑1 (MQ=255)

                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAAtt                                                                                                              >  1:2334031/1‑140 (MQ=255)

                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAAtt                                                                                                              >  2:928794/1‑140 (MQ=255)

                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAAtt                                                                                                              >  1:208588/1‑140 (MQ=255)

                       aTCGGCATGATTATCCGGTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATTAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAAtt                                                                                                              >  1:1913006/1‑140 (MQ=255)

                         cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTgc                                                                                                            >  1:1131764/1‑140 (MQ=255)

                         cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTgc                                                                                                            >  1:1751030/1‑140 (MQ=255)

                         cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑ATACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGGGAAGCACGTTGCTGACAAATTg                                                                                                             >  1:655777/1‑140 (MQ=255)

                         cGGCATGATTATCCGTTTCTGCAGGGTCTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAG‑AAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTgcg                                                                                                           >  2:2362596/1‑140 (MQ=255)

                             aTGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCtt                                                                                                        >  1:2124184/1‑140 (MQ=255)

                               gATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTa                                                                                                      >  2:2296344/1‑140 (MQ=255)

                                 ttttctgTTTCTGCGGGTTTTTTAATCGGACGATTAGTGGGTAAAAGGGGGGGTTTTTGGGGGG‑TTAAAGGTAATTCCCAGGCAAAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATg                                                                                                    <  2:1657773/134‑1 (MQ=255)

                                      cGTTTCTGCAGGTTTTTTAATGGGACGATTAGGGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGAAAATTCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGcc                                                                                               <  2:1751030/140‑1 (MQ=255)

                                       ttttCTGCGGGTTTTTTAATCGGACGATTGGGGGGTGAAATGGGGGGTTTTTTGGGGG‑TTACAGGAAAATTCCAGGAAAAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCg                                                                                              <  2:2232054/139‑1 (MQ=255)

                                         ttCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGa                                                                                            >  1:834249/1‑140 (MQ=255)

                                          tCTGCGGGGTTTTTAATCGGACGATTGGGGGGTAAAATGAGGGTTTATTTGGGGG‑TTACAGGAAAATCCCAGGCAAAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGAt                                                                                           <  2:1131764/140‑1 (MQ=255)

                                          tCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGAt                                                                                           >  1:1728219/1‑140 (MQ=255)

                                           cTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                          >  2:888262/1‑140 (MQ=255)

                                            tGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgc                                                                                         >  1:1195476/1‑140 (MQ=255)

                                              cAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcgg                                                                                       >  1:2230560/1‑140 (MQ=255)

                                                   tttttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaa                                                                                  <  2:1851447/140‑1 (MQ=255)

                                                   tttttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaa                                                                                  >  1:818864/1‑140 (MQ=255)

                                                   tttttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaa                                                                                  >  2:847318/1‑140 (MQ=255)

                                                     tttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATAGCGCTTTATGTTGCCGGATGCGGCGTAAAc                                                                                >  2:1475334/1‑140 (MQ=255)

                                                      ttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACg                                                                               >  1:1560054/1‑140 (MQ=255)

                                                      ttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACg                                                                               >  1:2267075/1‑140 (MQ=255)

                                                      ttAAGCGGACGATTAGTGGGTAAAATGGGGGTTTATTGGGGGG‑TTACGGATAATTTCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACg                                                                               <  2:2298003/140‑1 (MQ=255)

                                                           cGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTa                                                                          >  1:286223/1‑140 (MQ=255)

                                                             gACGATTGGTGGGTAAAATGGGGGTTTATTGGGGGG‑TTACAGATAAATCCCGGGAAAAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                        <  2:1728219/140‑1 (MQ=37)

                                                             gACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                        >  1:2298003/1‑140 (MQ=255)

                                                             agagATTAGGGGGAAAAAGGGGGGTTTATTTGGGGG‑TTAGAGG‑AAATTCCAGGGAAAAAAAAACCCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                        <  2:1913006/137‑1 (MQ=25)

                                                               ggATTGGTGGGTAAAAGGAGGGGTTATTGGGGGG‑TTACAGGTAAATCCCAGGCAAAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                      <  2:818864/139‑1 (MQ=38)

                                                               ggATTGGGGGGTAAAAGGGGGGTTTATTGGGGGG‑TTACAGGTAAATCCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                      <  2:373416/139‑1 (MQ=37)

                                                               ggATTAGGGGGTGAAATGGGGGGTTATTGGGGGG‑TTACAGGAAAATCCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                      <  2:208588/139‑1 (MQ=37)

                                                               gaaTTAGTGGGTAAAATGGGGGGTTTTTTGGGGG‑TTACAGGTAAATTCCAGGCAAAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                      <  1:1609264/138‑1 (MQ=38)

                                                               cGATTAGGGGGTAAAATGAGGGTTTATTGGGGGG‑TTAAAGGTAAATTCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                      <  1:1583813/140‑1 (MQ=37)

                                                                 tttggggggTAAAATGGGGGTTTTTTGGGGGG‑TTAAAGGTAAATCCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                    <  2:2255329/134‑1 (MQ=25)

                                                                        ggTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaa                                                             >  1:1400630/1‑140 (MQ=255)

                                                                           aaaaTGGGGGGTTATTTGGGGG‑TTACAGGAAAATTCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaaa                                                           <  2:655777/139‑1 (MQ=37)

                                                                             aaTGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt                                                        >  2:187471/1‑140 (MQ=255)

                                                                                 aGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCaa                                                    >  1:1986866/1‑140 (MQ=255)

                                                                                   gggTTTTTTGGGGGTTTACAGGAAAATCCCAGGCAAAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCaaa                                                   <  1:1718396/140‑1 (MQ=37)

                                                                                    ggTTATTGGGGGG‑TTACAGGTAAATCCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAtt                                                 <  1:1366334/140‑1 (MQ=37)

                                                                                        aTTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAAt                                             >  1:2241436/1‑140 (MQ=255)

                                                                                         tttGGGGG‑TTACAGGTAATTTCCAGGAAAAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATa                                            <  2:2266559/140‑1 (MQ=37)

                                                                                         tttGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATa                                            >  1:977888/1‑140 (MQ=255)

                                                                                         ttgggggg‑TTACAGGTATATTCCAGGCAAAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATa                                            <  2:2142036/137‑1 (MQ=37)

                                                                                           tGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATaaa                                          >  1:1896399/1‑140 (MQ=255)

                                                                                              ggg‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTg                                       >  1:1654466/1‑140 (MQ=255)

                                                                                               gg‑TTACAGGTAAATTCCAGGCAGAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGc                                      <  1:888262/140‑1 (MQ=255)

                                                                                               gg‑TTACAGATAAATCCCAGGAAAAAAAAAC‑CCCCGTCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGc                                      <  2:1659645/140‑1 (MQ=25)

                                                                                                g‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCa                                     >  2:1210044/1‑140 (MQ=255)

                                                                                                g‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCa                                     >  1:375689/1‑140 (MQ=255)

                                                                                                g‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCa                                     >  1:2288854/1‑140 (MQ=255)

                                                                                                    aCAGGTAAATCCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                                  <  2:2230560/140‑1 (MQ=37)

                                                                                                      aGGAAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCa                                <  2:2267075/140‑1 (MQ=255)

                                                                                                           aaTTCCAGGCAAAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                           <  2:471221/140‑1 (MQ=255)

                                                                                                                cAGGCAAAAAAAAC‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGc                      <  2:1407499/140‑1 (MQ=255)

                                                                                                                  ggCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGACTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCt                    >  2:51471/1‑140 (MQ=255)

                                                                                                                   gCAGAAAAAAA‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg                   >  2:1627247/1‑140 (MQ=255)

                                                                                                                     aaaaaaaac‑CCCCGCCGGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGAt                 <  1:1475334/138‑1 (MQ=255)

                                                                                                                                  cgccgGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAgcgcagcgca     >  2:103202/1‑140 (MQ=255)

                                                                                                                                     cgGTGGCG‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCa  <  2:185988/140‑1 (MQ=255)

AACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGC‑‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCA  >  NC_000913/2001794‑2002063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A