Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,304,212	G→A	G255G (GGG→GGA)	yhbW →	putative luciferase‑like monooxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,304,212	0	G	A	99.2%	433.2 / NA	129	G255G (GGG→GGA)	yhbW	putative luciferase‑like monooxygenase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (53/75); minor base C (0/1); total (53/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

AACCGTCAGCACGGCTGGAAAAACCATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGGCAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGAAAGTGCGTCATGGCTTGCAGTCGATCCT  >  NC_000913/3304076‑3304343

aaCCGTCAGCACGGCTGGAAAAACCATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACaa                                                                                                                                  >  2:1035922/1‑140 (MQ=255)

  ccGTCAGCACGGCTGGAAAAACCATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACt                                                                                                                                >  2:1410286/1‑140 (MQ=255)

    gTCAGCACGGCTGGAAAAACCATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCTTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTgc                                                                                                                              <  1:81695/140‑1 (MQ=255)

     tCAGCACGGCTGGAAAAACCATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACaa                                                                                                                                  >  1:220014/1‑135 (MQ=255)

     tCAGCACGGCTGGAAAAACCATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACaa                                                                                                                                  <  2:220014/135‑1 (MQ=255)

     tCAGCACGGCTGGAAAAACCATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTgcc                                                                                                                             >  2:311010/1‑140 (MQ=255)

     tCAGCACGGCTGGAAAAACCATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTgcc                                                                                                                             >  1:374350/1‑140 (MQ=255)

     tCAGCACGGCTGGAAAAACCATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTgcc                                                                                                                             >  1:1923141/1‑140 (MQ=255)

      cAGCACGGCTGGAAAAACCATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTgccg                                                                                                                            >  2:1735932/1‑140 (MQ=255)

         cACGGCTGGAAAAACCATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTgccgccg                                                                                                                         >  1:2248676/1‑140 (MQ=255)

         cACGGCTGGAAAAACCATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTgccgccg                                                                                                                         <  1:947076/140‑1 (MQ=255)

           cGGCTGGAAAAACCATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTgccgccgcc                                                                                                                       <  1:1719109/140‑1 (MQ=255)

           cGGCTGGAAAAACCATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTgccgccgcc                                                                                                                       <  1:1925466/140‑1 (MQ=255)

             gCTGGAAAAACCATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGa                                                                                                                     <  2:1640964/140‑1 (MQ=255)

                 gAAAAACCATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCa                                                                                                                 <  2:1230987/140‑1 (MQ=255)

                 gAAAAACCATACGCGATGGTGTGCATCAATATTATCGCCGCCCACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCa                                                                                                                 <  2:2248676/140‑1 (MQ=255)

                      aCCATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAata                                                                                                            <  1:714112/140‑1 (MQ=255)

                      aCCATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAata                                                                                                            <  2:374350/140‑1 (MQ=255)

                       ccATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGAtt                                                                                                                   <  1:528472/132‑1 (MQ=255)

                       ccATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGAtt                                                                                                                   >  2:528472/1‑132 (MQ=255)

                           aCGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTCCCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGAt                                                                                                       <  2:1488936/140‑1 (MQ=255)

                           aCGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCTGCCGCCGATTCAAAATATGGAt                                                                                                       <  2:99296/140‑1 (MQ=255)

                           aCGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGAt                                                                                                       <  1:278014/140‑1 (MQ=255)

                           aCGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGAt                                                                                                       <  2:1361286/140‑1 (MQ=255)

                              cgATGGTGTGCATCAATATTATCGCCTCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAg                                                                                                    <  2:860084/140‑1 (MQ=255)

                               gATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGt                                                                                                   <  2:1984196/140‑1 (MQ=255)

                                aTGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGtt                                                                                                  >  2:1911150/1‑140 (MQ=255)

                                      tgCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTc                                                                                            >  2:60062/1‑140 (MQ=255)

                                      tgCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTc                                                                                            <  2:1306555/140‑1 (MQ=255)

                                      tgCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTc                                                                                            <  1:98466/140‑1 (MQ=255)

                                        cATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCAc                                                                                          <  2:403799/140‑1 (MQ=255)

                                           cAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGAt                                                                                                       >  2:322222/1‑124 (MQ=255)

                                           cAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGAt                                                                                                       <  1:322222/124‑1 (MQ=255)

                                           cAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGt                                                                                       <  1:230733/140‑1 (MQ=255)

                                           cAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGt                                                                                       >  2:3788/1‑140 (MQ=255)

                                           cAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGt                                                                                       <  2:1959800/140‑1 (MQ=255)

                                             atatTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGt                                                                                       <  1:654029/138‑1 (MQ=255)

                                             atatTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGt                                                                                       >  2:654029/1‑138 (MQ=255)

                                             atatTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCt                                                                                     <  2:484301/140‑1 (MQ=255)

                                             atatTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCt                                                                                     <  1:2057840/140‑1 (MQ=255)

                                                ttatCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCt                                                                                     >  2:2169520/1‑137 (MQ=255)

                                                ttatCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAg                                                                                  <  2:191725/140‑1 (MQ=255)

                                                ttatCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAg                                                                                  <  2:1943781/140‑1 (MQ=255)

                                                ttatCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAg                                                                                  <  1:169910/140‑1 (MQ=255)

                                                  atCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCa                                                                                >  2:1602430/1‑140 (MQ=255)

                                                  atCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCa                                                                                <  1:1445038/140‑1 (MQ=255)

                                                   tCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAg                                                                               >  1:2061744/1‑140 (MQ=255)

                                                        gccgACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATgg                                                                          >  1:1834065/1‑140 (MQ=255)

                                                        gccgACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATgg                                                                          >  2:1762170/1‑140 (MQ=255)

                                                                cAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTgcagc                                                                  <  1:111672/140‑1 (MQ=255)

                                                                   ccGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAgg                                                               >  2:739816/1‑140 (MQ=255)

                                                                    cgcgACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGgc                                                              <  2:904495/140‑1 (MQ=255)

                                                                    cgcgACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGgc                                                              <  2:1476599/140‑1 (MQ=255)

                                                                    cgcgACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGgc                                                              <  2:920332/140‑1 (MQ=255)

                                                                     gcgACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGgcg                                                             <  1:1273458/140‑1 (MQ=255)

                                                                      cgACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGgcgc                                                            <  1:2183478/140‑1 (MQ=255)

                                                                       gACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGCCAACGGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCt                                                           <  1:610836/140‑1 (MQ=255)

                                                                           cTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGt                                                       <  1:943561/140‑1 (MQ=255)

                                                                           cTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGt                                                       <  1:1017006/140‑1 (MQ=255)

                                                                           cTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGt                                                       <  2:1112092/140‑1 (MQ=255)

                                                                           cTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGt                                                       <  1:3788/140‑1 (MQ=255)

                                                                              aaTTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATg                                                    <  1:1911150/140‑1 (MQ=255)

                                                                                       ttACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTa                                           <  1:739816/140‑1 (MQ=255)

                                                                                        tACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGGTTCAAAATATGGATCAGTTCTGGTCACCGTCTTAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAg                                          >  2:2240662/1‑140 (MQ=255)

                                                                                        tACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAg                                          >  1:1047475/1‑140 (MQ=255)

                                                                                        tACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAg                                          >  1:443575/1‑140 (MQ=255)

                                                                                        tACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAg                                          >  2:822147/1‑140 (MQ=255)

                                                                                        tACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAg                                          >  2:1063578/1‑140 (MQ=255)

                                                                                            tCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGa                                      <  2:570765/140‑1 (MQ=255)

                                                                                               aTGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATaa                                   >  2:1292209/1‑140 (MQ=255)

                                                                                               aTGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATaa                                   >  2:656324/1‑140 (MQ=255)

                                                                                                tGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATaaa                                  <  2:482814/140‑1 (MQ=255)

                                                                                                 gcagcaAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAg                                 <  2:1834065/140‑1 (MQ=255)

                                                                                                 gcagcaAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAg                                 <  1:822147/140‑1 (MQ=255)

                                                                                                     caAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAg                                          <  1:656488/127‑1 (MQ=255)

                                                                                                     caAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAg                                          >  2:656488/1‑127 (MQ=255)

                                                                                                     caAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGaa                             >  1:251328/1‑140 (MQ=255)

                                                                                                     caAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGaa                             >  1:49561/1‑140 (MQ=255)

                                                                                                      aaGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGaaa                            <  1:60062/140‑1 (MQ=255)

                                                                                                        gCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGAAAgg                          >  2:1358798/1‑139 (MQ=255)

                                                                                                        gCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGAAAGt                          >  1:1137076/1‑140 (MQ=255)

                                                                                                        gCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGAAAGt                          <  2:2061744/140‑1 (MQ=255)

                                                                                                          cTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATa                                    <  2:2060768/128‑1 (MQ=255)

                                                                                                          cTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATa                                    >  1:2060768/1‑128 (MQ=255)

                                                                                                          cTTTGTGAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGAAAGTGc                        >  2:400022/1‑140 (MQ=255)

                                                                                                             tgtgAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCTCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGAAAGTGCGTc                     >  1:1373891/1‑140 (MQ=255)

                                                                                                             tgtgAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGAAAGTGCGTc                     <  2:1137076/140‑1 (MQ=255)

                                                                                                             tgtgAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGAAAGTGCGTc                     <  1:747466/140‑1 (MQ=255)

                                                                                                              gtgAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGAAAGTGCGTCa                    <  1:1063578/140‑1 (MQ=255)

                                                                                                              gtgAAGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGACCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGAAAGTGCGTCa                    <  1:1735932/140‑1 (MQ=255)

                                                                                                                  aGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGAAAGTGCGTCATGGc                <  1:57179/140‑1 (MQ=255)

                                                                                                                  aGCTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGATCACCGTCTGAGCAGTAGGGCGTGCAGGAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGAGAAAGTGCGTCATGGc                >  2:2157622/1‑140 (MQ=255)

                                                                                                                    cTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGa                              <  1:674109/124‑1 (MQ=255)

                                                                                                                    cTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGa                              >  2:674109/1‑124 (MQ=255)

                                                                                                                    cTGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATACAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGAAAGTGCGTCATGGCtt              <  1:1035922/140‑1 (MQ=255)

                                                                                                                     tGCGCCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGAAAGTGCGTCATGGCTTg             >  2:535821/1‑140 (MQ=255)

                                                                                                                       cgcCGTGGCGAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGAAAGTGCGTCATGGCTTGCa           >  2:287596/1‑140 (MQ=255)

                                                                                                                         ccGTGGCGAAACCGGACAACTGCCGCCTCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGAAAGTGCGTCATGGCTTGCAGt         <  2:1373891/140‑1 (MQ=255)

                                                                                                                                gAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGAAAGTGCGTCATGGCTTGCAGTCGATCCt  <  1:535821/140‑1 (MQ=255)

                                                                                                                                gAAACCGGACAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGAAAGTGCGTCATGGCTTGCAGTCGATCCt  <  1:1410286/140‑1 (MQ=255)

AACCGTCAGCACGGCTGGAAAAACCATACGCGATGGTGTGCATCAATATTATCGCCGCCGACAGCAACCGCGACGCTGAATTTCTGTTTACCTCAATGCAGCAAGCCTTTGTGAAGCTGCGCCGTGGCGAAACCGGGCAACTGCCGCCGCCGATTCAAAATATGGATCAGTTCTGGTCACCGTCTGAGCAGTATGGCGTGCAGCAGGCGCTGAGTATGTCGTTGGTAGGTGATAAAGCGAAAGTGCGTCATGGCTTGCAGTCGATCCT  >  NC_000913/3304076‑3304343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A