Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,304,813	(C)_7→8	coding (1005/1245 nt)	mtr ←	tryptophan:H(+) symporter Mtr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,304,806	1	.	C	97.0%	340.5 / 1.9	100	coding (1012/1245 nt)	mtr	tryptophan:H(+) symporter Mtr
Reads supporting (aligned to +/- strand): ref base . (3/0); new base C (56/40); total (60/40)
Fisher's exact test for biased strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 111 total aligned reads displayed.

CACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑G‑‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCG  >  NC_000913/3304668‑3304940

cACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑G‑‑‑c                                                                                                                                        >  2:370949/1‑140 (MQ=255)

 cccccAGACGCGGAATTTCGGGCTGCCAAACCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑G‑‑‑cc                                                                                                                                       <  2:1684582/139‑1 (MQ=255)

 aCCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑G‑‑‑cc                                                                                                                                       <  2:588662/140‑1 (MQ=255)

 aCCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑G‑‑‑cc                                                                                                                                       <  1:370949/140‑1 (MQ=255)

    ccAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑G‑‑‑ccccc                                                                                                                                    <  2:1900646/140‑1 (MQ=255)

     cAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑G‑‑‑cccccc                                                                                                                                   <  1:1765661/140‑1 (MQ=255)

      aGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑G‑‑‑ccccccc                                                                                                                                  <  1:884801/140‑1 (MQ=255)

        aCGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑G‑‑‑cccccccca                                                                                                                                >  2:235746/1‑138 (MQ=255)

          gcgGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑G‑‑‑ccccccccaca                                                                                                                              >  2:1413002/1‑136 (MQ=255)

            ggAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAAc                                                                                                                            >  1:2040127/1‑140 (MQ=255)

             gAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACt                                                                                                                           >  1:2110350/1‑140 (MQ=255)

               aTTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTgg                                                                                                                         <  1:889703/140‑1 (MQ=255)

                 ttCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCg                                                                                                                       <  1:1413002/140‑1 (MQ=255)

                      gCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCa                                                                                                                  <  2:931710/140‑1 (MQ=255)

                      gCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCa                                                                                                                  <  1:1161019/140‑1 (MQ=255)

                      gCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCa                                                                                                                  <  1:1771639/140‑1 (MQ=255)

                       cTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GCC‑CCCCCCC‑ACAACGGGCGGGGCa                                                                                                                  <  1:1703289/140‑1 (MQ=255)

                        tGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCaaa                                                                                                                >  1:93786/1‑140 (MQ=255)

                         gCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAg                                                                                                               <  2:247293/140‑1 (MQ=255)

                                   ttACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATg                                                                                                     <  1:2131692/140‑1 (MQ=255)

                                   ttACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATg                                                                                                     <  2:2403390/140‑1 (MQ=255)

                                   ttACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATg                                                                                                     <  1:2025717/140‑1 (MQ=255)

                                           tGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTc                                                                                             >  2:1067707/1‑140 (MQ=255)

                                              aCGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAg                                                                                          <  2:1484617/140‑1 (MQ=255)

                                              aCGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAg                                                                                          <  1:983049/140‑1 (MQ=255)

                                                   cTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGcc                                                                                     <  1:1304036/140‑1 (MQ=255)

                                                     aaCAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAg                                                                                          >  2:126999/1‑133 (MQ=255)

                                                     aaCAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAg                                                                                          <  1:126999/133‑1 (MQ=255)

                                                     aaCAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCca                                                                                   >  2:1906393/1‑140 (MQ=255)

                                                     aaCAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCca                                                                                   >  1:1409808/1‑140 (MQ=255)

                                                       cAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCcaca                                                                                 >  1:1026917/1‑140 (MQ=255)

                                                       cAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCcaca                                                                                 >  2:52801/1‑140 (MQ=255)

                                                          cgcCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGcc                                                                              >  1:2216683/1‑140 (MQ=255)

                                                            ccGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGa                                                                            >  2:283530/1‑140 (MQ=255)

                                                            ccGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGa                                                                            >  2:1569385/1‑140 (MQ=255)

                                                            ccGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGa                                                                            >  2:1627177/1‑140 (MQ=255)

                                                            ccGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGa                                                                            >  1:205971/1‑140 (MQ=255)

                                                            ccGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGa                                                                            >  1:1775426/1‑140 (MQ=255)

                                                            ccGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGa                                                                            >  1:1418793/1‑140 (MQ=255)

                                                            ccGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGa                                                                            >  2:1266635/1‑140 (MQ=255)

                                                            ccGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGACAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGa                                                                            >  2:2128258/1‑140 (MQ=255)

                                                                aaCAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GCC‑CCCCCCC‑ACAACTGGCGGGGCAAAAGGCAGGAATGCCGTTTTCAAGCGGCCCACAGCCGAgcc                                                                         >  2:1198316/1‑138 (MQ=255)

                                                                 aCAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAgtcgt                                                                       <  2:1409808/140‑1 (MQ=255)

                                                                 aCAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAgtcgt                                                                       <  1:539632/140‑1 (MQ=255)

                                                                      tGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GCC‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGaa                                                                   <  2:1466279/140‑1 (MQ=255)

                                                                      tGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGaaa                                                                  <  2:2185271/140‑1 (MQ=255)

                                                                      tGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGaaa                                                                  <  1:496844/140‑1 (MQ=255)

                                                                          gccCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCaa                                                              <  2:93786/140‑1 (MQ=255)

                                                                          gccCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCaa                                                              >  1:97772/1‑140 (MQ=255)

                                                                          gccCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCaa                                                              <  2:225627/140‑1 (MQ=255)

                                                                           cccAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GCC‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAGGCGGCCCACAGCCGAGTCGTCGAAACCaa                                                              <  1:1198316/140‑1 (MQ=255)

                                                                           cccAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCaaa                                                             <  1:804801/140‑1 (MQ=255)

                                                                            ccAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGTGGCCCACAGCCGAGTCGTCGAAACCAAAc                                                            >  1:1093542/1‑140 (MQ=255)

                                                                             cAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCGGTTCGGGACCAACA‑C‑‑‑CCCCCCCAACAACTGGCGGGGAAAAGGCCGCCAATGCCGTTTTCAAGCGGCCCAAGCCCGAGCCGCCGAAACCaacaa                                                           >  2:938857/1‑137 (MQ=255)

                                                                              aGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAg                                                          <  2:175964/140‑1 (MQ=255)

                                                                              aGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAg                                                          <  2:1827424/140‑1 (MQ=255)

                                                                              aGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAg                                                          <  2:991008/140‑1 (MQ=255)

                                                                              aGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAg                                                          <  1:1266950/140‑1 (MQ=255)

                                                                                aTGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGAt                                                        >  1:1124186/1‑140 (MQ=255)

                                                                                aTGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGAt                                                        <  2:2040127/140‑1 (MQ=255)

                                                                                     aGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGcc                                                   >  1:629752/1‑140 (MQ=255)

                                                                                      gCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGTGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCa                                                  <  2:1093542/140‑1 (MQ=255)

                                                                                         gCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GCCCCCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAg                                                 >  2:76787/1‑140 (MQ=255)

                                                                                          cTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATa                                              >  1:2303585/1‑140 (MQ=255)

                                                                                          cTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATa                                              >  2:17532/1‑140 (MQ=255)

                                                                                            aaaCCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGt                                            <  2:2213530/140‑1 (MQ=255)

                                                                                                cAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTcaaa                                        <  2:1418793/140‑1 (MQ=255)

                                                                                                  gCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTcaaaca                                      >  1:1125266/1‑140 (MQ=255)

                                                                                                   cATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTcaaacaa                                     >  1:2323268/1‑140 (MQ=255)

                                                                                                   cATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTcaaacaa                                     >  1:564774/1‑140 (MQ=255)

                                                                                                    aTAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTcaaacaaa                                    >  2:1825811/1‑140 (MQ=255)

                                                                                                       aCCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAAccc                                 >  2:2167317/1‑140 (MQ=255)

                                                                                                       aCCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAAccc                                 >  2:2074880/1‑140 (MQ=255)

                                                                                                            tGGCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGt                            >  2:1890194/1‑140 (MQ=255)

                                                                                                             ggCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGtt                           >  2:1456282/1‑140 (MQ=255)

                                                                                                             ggCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGtt                           >  2:942671/1‑140 (MQ=255)

                                                                                                             ggCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGtt                           >  1:1685945/1‑140 (MQ=255)

                                                                                                             ggCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGtt                           >  2:368792/1‑140 (MQ=255)

                                                                                                             ggCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGtt                           >  2:1708564/1‑140 (MQ=255)

                                                                                                              gCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTa                          >  2:590415/1‑140 (MQ=255)

                                                                                                              gCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTa                          <  1:1890194/140‑1 (MQ=255)

                                                                                                              gCGTACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTG‑CAGATAGTCAAACAAACCCAGCGTTAc                         >  2:2331635/1‑140 (MQ=255)

                                                                                                                 tACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGc                       >  2:695438/1‑140 (MQ=255)

                                                                                                                 tACAGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGc                       >  2:1062817/1‑140 (MQ=255)

                                                                                                                    aGGAATCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGa                    <  1:19374/140‑1 (MQ=255)

                                                                                                                       aaTCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGCCAAACAAACCCAGCGTTACGCCGAGga                 <  1:551362/140‑1 (MQ=255)

                                                                                                                        aTCCGTTCGGGAACAACACC‑‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaa                >  1:1175862/1‑140 (MQ=255)

                                                                                                                        aTCCGTTCGGGAACAACA‑GT‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGGCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaa                >  2:2109661/1‑140 (MQ=255)

                                                                                                                         tCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaac               >  2:1724793/1‑140 (MQ=255)

                                                                                                                         tCCGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaac               <  1:938857/140‑1 (MQ=255)

                                                                                                                           cGTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaacga             >  1:909177/1‑140 (MQ=255)

                                                                                                                            gTTCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaacgaa            <  1:2167317/140‑1 (MQ=255)

                                                                                                                             ttCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaacgaac           >  2:1156957/1‑140 (MQ=255)

                                                                                                                              tCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACt          >  1:1384722/1‑140 (MQ=255)

                                                                                                                              tCGGGAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACt          >  1:903930/1‑140 (MQ=255)

                                                                                                                                gggAACAACA‑GCC‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTc         >  2:509452/1‑140 (MQ=255)

                                                                                                                                gggAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCg        <  2:2021628/140‑1 (MQ=255)

                                                                                                                                gggAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCg        <  1:590415/140‑1 (MQ=255)

                                                                                                                                gggAACAACA‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCg        >  2:1128966/1‑140 (MQ=255)

                                                                                                                                      aaca‑GC‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCg  >  2:129432/1‑140 (MQ=255)

CACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACA‑G‑‑‑CCCCCCC‑ACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCG  >  NC_000913/3304668‑3304940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A