Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F28 I1 R1 258 142.3 4824480 99.1% 4781059 138.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,423,458 C→T noncoding (85/120 nt) rrfF ← 5S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,423,4580CT96.8% 302.7 / ‑0.2 94noncoding (85/120 nt)rrfF5S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base T (58/33);  total (61/33)
Fisher's exact test for biased strand distribution p-value = 5.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.13e-01

CGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCA  >  NC_000913/3423324‑3423594
                                                                                                                                      |                                                                                                                                        
cGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCcaca                                                                                                                                     >  2:428838/1‑140 (MQ=255)
  cTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACt                                                                                                                                   >  1:338442/1‑140 (MQ=255)
       ggcggATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCAt                                                                                                                              >  2:672655/1‑140 (MQ=255)
            aTTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGgcg                                                                                                                         >  1:899694/1‑140 (MQ=255)
                      cTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGtt                                                                                                               >  1:739952/1‑140 (MQ=255)
                         gggAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCa                                                                                                            >  1:358210/1‑140 (MQ=255)
                           gagaGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACt                                                                                                          >  1:1723209/1‑140 (MQ=255)
                              agTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCt                                                                                                       <  1:1491131/140‑1 (MQ=255)
                              agTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCt                                                                                                       >  1:85312/1‑140 (MQ=255)
                                tgtTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGa                                                                                                     >  1:143896/1‑140 (MQ=255)
                                tgtTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGa                                                                                                     >  1:1126230/1‑140 (MQ=255)
                                 gtTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAg                                                                                                    >  1:108866/1‑140 (MQ=255)
                                  ttCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGt                                                                                                   >  1:2373253/1‑140 (MQ=255)
                                       cGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGc                                                                                              >  2:2334427/1‑140 (MQ=255)
                                        gACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCa                                                                                             <  2:358210/140‑1 (MQ=255)
                                        gACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCa                                                                                             >  1:2349627/1‑140 (MQ=255)
                                         acaaacaaCAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAt                                                                                            <  2:108866/140‑1 (MQ=255)
                                          caaacaaCAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATg                                                                                           >  1:2346424/1‑140 (MQ=255)
                                          caaacaaCAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATg                                                                                           >  2:1400192/1‑140 (MQ=255)
                                          caaacaaCAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATg                                                                                           >  1:1220133/1‑140 (MQ=25)
                                             acaacaGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATgggg                                                                                        <  2:143896/140‑1 (MQ=255)
                                              caacaGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGt                                                                                       <  2:899694/140‑1 (MQ=255)
                                                acaGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCa                                                                                     <  1:2334427/140‑1 (MQ=255)
                                                 caGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAg                                                                                    <  2:739952/140‑1 (MQ=255)
                                                    aTAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTg                                                                                 >  2:14468/1‑140 (MQ=255)
                                                    aTAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTg                                                                                 >  2:1700677/1‑140 (MQ=255)
                                                    aTAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTg                                                                                 >  2:223164/1‑140 (MQ=255)
                                                    aTAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTg                                                                                 >  1:1404626/1‑140 (MQ=255)
                                                        aaCAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGac                                                                             <  2:1404626/140‑1 (MQ=255)
                                                        aaCAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGac                                                                             >  1:996284/1‑140 (MQ=255)
                                                        aaCAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGac                                                                             <  2:996284/140‑1 (MQ=255)
                                                        aaCAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGac                                                                             <  2:2346424/140‑1 (MQ=255)
                                                        aaCAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGac                                                                             <  2:2373253/140‑1 (MQ=255)
                                                        aaCAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGac                                                                             <  1:2381195/140‑1 (MQ=255)
                                                           aaaaGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGaccac                                                                          >  2:556562/1‑140 (MQ=255)
                                                             aaGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcg                                                                        >  1:2170799/1‑140 (MQ=255)
                                                                  ccAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTa                                                                   >  1:252864/1‑140 (MQ=37)
                                                                          ccGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTgccgcc                                                           >  2:1555168/1‑140 (MQ=37)
                                                                          ccGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTgccgcc                                                           >  2:1494232/1‑140 (MQ=37)
                                                                          ccGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTgccgcc                                                           <  2:2349627/140‑1 (MQ=25)
                                                                          ccGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTgccgcc                                                           >  2:497337/1‑140 (MQ=37)
                                                                               tGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACa                                                      >  2:2137314/1‑140 (MQ=255)
                                                                                gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgcg                                                                      <  2:2214662/123‑1 (MQ=25)
                                                                                gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgcg                                                                      >  1:2214662/1‑123 (MQ=37)
                                                                                gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCaa                                                     >  2:1596833/1‑140 (MQ=18)
                                                                                gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCaa                                                     >  2:2336627/1‑140 (MQ=255)
                                                                                 aGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACaaa                                                    <  1:1741097/140‑1 (MQ=38)
                                                                                  gCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAAt                                                   <  1:1596833/140‑1 (MQ=9)
                                                                                  gCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAAt                                                   <  1:14468/140‑1 (MQ=9)
                                                                                   ccTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAAtt                                                  <  1:497337/140‑1 (MQ=38)
                                                                                   ccTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAAtt                                                  <  2:85312/140‑1 (MQ=38)
                                                                                      tttGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCtt                                               <  1:546278/140‑1 (MQ=38)
                                                                                           tttATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTa                                          <  1:556562/140‑1 (MQ=255)
                                                                                             tATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAAt                                        >  2:2155635/1‑140 (MQ=255)
                                                                                                ttGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTg                                     >  1:198557/1‑140 (MQ=255)
                                                                                                    tGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGa                                 >  2:398987/1‑140 (MQ=255)
                                                                                                    tGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGa                                 >  1:1919093/1‑140 (MQ=255)
                                                                                                    tGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGa                                 >  1:180591/1‑140 (MQ=255)
                                                                                                    tGTCTGGCAGTTCCCTACTCTAGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGa                                 >  2:1125316/1‑140 (MQ=255)
                                                                                                        tGGCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACtt                             >  1:1655962/1‑140 (MQ=255)
                                                                                                         ggCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACttt                            >  2:665612/1‑140 (MQ=255)
                                                                                                         ggCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACttt                            <  1:1494232/140‑1 (MQ=255)
                                                                                                         ggCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACttt                            >  1:1124250/1‑140 (MQ=255)
                                                                                                         ggCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACttt                            >  1:2047173/1‑140 (MQ=255)
                                                                                                         ggCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACttt                            >  1:2318815/1‑140 (MQ=255)
                                                                                                         ggCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACtgt                            >  2:2231027/1‑138 (MQ=255)
                                                                                                          gCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTa                           <  1:322293/140‑1 (MQ=255)
                                                                                                          gCAGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTa                           <  1:1408643/140‑1 (MQ=255)
                                                                                                            aGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAAc                         <  2:2047173/140‑1 (MQ=255)
                                                                                                            aGTTCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAAc                         <  2:2170799/140‑1 (MQ=255)
                                                                                                              ttCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCt                       >  2:1187804/1‑140 (MQ=255)
                                                                                                              ttCCCTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCt                       >  2:1117937/1‑140 (MQ=255)
                                                                                                                cccTACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTaa                     >  1:989163/1‑140 (MQ=255)
                                                                                                                   tACTCTCGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTaaaaa                  >  2:727542/1‑140 (MQ=255)
                                                                                                                       ctcGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtgg              <  1:1717801/140‑1 (MQ=255)
                                                                                                                       ctcGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtgg              <  2:1126230/140‑1 (MQ=255)
                                                                                                                       ctcGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtgg              <  1:1400192/140‑1 (MQ=255)
                                                                                                                       ctcGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtg               >  1:343463/1‑139 (MQ=255)
                                                                                                                       ctcGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtg               <  2:343463/139‑1 (MQ=255)
                                                                                                                        tcGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtggt             >  2:269992/1‑140 (MQ=255)
                                                                                                                        tcGCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtggt             >  2:1653489/1‑140 (MQ=255)
                                                                                                                          gCATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGc           >  2:1400417/1‑140 (MQ=255)
                                                                                                                           cATGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCt          >  1:114958/1‑140 (MQ=255)
                                                                                                                            aTGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTg         >  2:438020/1‑140 (MQ=255)
                                                                                                                             tGGGGAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGa        >  2:24646/1‑140 (MQ=255)
                                                                                                                               gggAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATa      >  2:827808/1‑140 (MQ=255)
                                                                                                                               gggAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATa      >  2:922548/1‑140 (MQ=255)
                                                                                                                                ggAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATAc     >  1:828032/1‑140 (MQ=255)
                                                                                                                                ggAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATAc     >  1:479957/1‑140 (MQ=255)
                                                                                                                                ggAGACTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATAc     >  1:1165786/1‑140 (MQ=255)
                                                                                                                                 gagaCTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATAcc    <  2:252864/140‑1 (MQ=255)
                                                                                                                                  agaCTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTg         >  2:488357/1‑134 (MQ=255)
                                                                                                                                  agaCTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTg         <  1:488357/134‑1 (MQ=255)
                                                                                                                                   gaCTCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCa  <  1:665612/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                        
CGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCA  >  NC_000913/3423324‑3423594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A