Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,908,180	A→G	Y63H (TAC→CAC)	pstB ←	phosphate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,908,180	0	A	G	100.0%	416.8 / NA	118	Y63H (TAC→CAC)	pstB	phosphate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (64/54); total (64/54)

Only 100 of 120 total aligned reads displayed.

GATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATGTTTTTCAGGGCATGGAATTTGCCGTAGTAG  >  NC_000913/3908046‑3908313

gATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGtt                                                                                                                                  <  1:2294596/140‑1 (MQ=255)

  tGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCa                                                                                                                                >  1:1390980/1‑140 (MQ=255)

   ggACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCaa                                                                                                                               <  2:895002/140‑1 (MQ=255)

     aCATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAAc                                                                                                                             >  2:797499/1‑140 (MQ=255)

     aCATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAAc                                                                                                                             >  1:2351869/1‑140 (MQ=255)

     aCATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAAc                                                                                                                             >  1:502796/1‑140 (MQ=255)

        tCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACAtt                                                                                                                          >  1:1577897/1‑140 (MQ=255)

        tCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACAtt                                                                                                                          >  2:289422/1‑140 (MQ=255)

         cGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACAttt                                                                                                                         >  2:2180830/1‑140 (MQ=255)

            aaaCGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTttgt                                                                                                                      <  2:2063656/140‑1 (MQ=255)

               cGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGa                                                                                                                   <  1:2206954/140‑1 (MQ=255)

               cGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGa                                                                                                                   <  2:1772703/140‑1 (MQ=255)

               cGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGa                                                                                                                   <  2:387330/140‑1 (MQ=255)

                 gCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAg                                                                                                                 <  2:655421/140‑1 (MQ=255)

                 gCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAg                                                                                                                 <  2:5655/140‑1 (MQ=255)

                      ggTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACg                                                                                                            <  2:1401025/140‑1 (MQ=255)

                       gTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACgc                                                                                                           >  2:1290763/1‑140 (MQ=255)

                            tGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTCCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGcagcag                                                                                                      >  2:400679/1‑140 (MQ=255)

                                    cATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGAtt                                                                                              >  2:521459/1‑140 (MQ=255)

                                       gCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTAc                                                                                           <  1:1834384/140‑1 (MQ=255)

                                           aCTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCa                                                                                       >  2:2075155/1‑140 (MQ=255)

                                             tttCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGc                                                                                     >  2:535226/1‑140 (MQ=255)

                                              ttCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAGCATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGcc                                                                                    >  1:2311452/1‑140 (MQ=255)

                                                 gcacgcaGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGa                                                                                 <  1:1455733/140‑1 (MQ=255)

                                                 gcacgcaGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGa                                                                                 <  2:1177160/140‑1 (MQ=255)

                                                  cacgcaGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGa                                                                                                >  2:2254895/1‑124 (MQ=255)

                                                  cacgcaGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGa                                                                                                <  1:2254895/124‑1 (MQ=255)

                                                  cacgcaGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGAc                                                                                >  2:121246/1‑140 (MQ=255)

                                                       agcagTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGccc                                                                           >  2:2368501/1‑140 (MQ=255)

                                                         cagTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGc                                                                             >  1:2084003/1‑136 (MQ=255)

                                                         cagTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGc                                                                             <  2:2084003/136‑1 (MQ=255)

                                                           gTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATa                                                                       <  2:502796/140‑1 (MQ=255)

                                                            tGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATaa                                                                      <  1:2368501/140‑1 (MQ=255)

                                                            tGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATaa                                                                      <  2:154667/140‑1 (MQ=255)

                                                            tGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATaa                                                                      <  2:446914/140‑1 (MQ=255)

                                                              cGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAAc                                                                    >  1:451236/1‑140 (MQ=255)

                                                               gATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACg                                                                   >  1:2091056/1‑140 (MQ=255)

                                                                 tatCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGcc                                                                 >  1:239729/1‑140 (MQ=255)

                                                                 tatCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGcc                                                                 >  1:265311/1‑140 (MQ=255)

                                                                  atCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCg                                                                <  1:2134667/140‑1 (MQ=255)

                                                                   tCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGt                                                               >  1:2392221/1‑140 (MQ=255)

                                                                   tCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGt                                                               >  1:313094/1‑140 (MQ=255)

                                                                   tCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGt                                                               >  1:1192186/1‑140 (MQ=255)

                                                                      tGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGc                                                                             >  1:1065021/1‑123 (MQ=255)

                                                                      tGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGc                                                                             <  2:1065021/123‑1 (MQ=255)

                                                                        agagTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCt                                                          <  1:121246/140‑1 (MQ=255)

                                                                         gagTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTg                                                         <  2:2052009/140‑1 (MQ=255)

                                                                         gagTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTg                                                         <  2:1298107/140‑1 (MQ=255)

                                                                         gagTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTg                                                         <  1:535226/140‑1 (MQ=255)

                                                                          agTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTgg                                                        >  2:912818/1‑140 (MQ=255)

                                                                          agTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTgg                                                        >  2:1280150/1‑140 (MQ=255)

                                                                          agTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTgg                                                        >  1:1456485/1‑140 (MQ=255)

                                                                             tGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCCCCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGttt                                                     <  2:1919708/140‑1 (MQ=255)

                                                                             tGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGttt                                                     >  2:1129118/1‑140 (MQ=255)

                                                                             tGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGCCCCGATAAACGCCGTTACCTGGttt                                                     >  1:1691424/1‑140 (MQ=255)

                                                                             tGGTCAGGATGTTGTCGCCATCAAGCAAAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGttt                                                     >  2:299214/1‑140 (MQ=255)

                                                                              ggTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGtttt                                                    >  2:695763/1‑140 (MQ=255)

                                                                              ggTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGtttt                                                    <  1:912818/140‑1 (MQ=255)

                                                                              ggTCAGGATGTGGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGtttt                                                    <  2:2351869/140‑1 (MQ=255)

                                                                               gTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGttttt                                                   >  1:1586804/1‑140 (MQ=255)

                                                                               gTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGttttt                                                   >  1:1847016/1‑140 (MQ=255)

                                                                               gTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGttttt                                                   >  2:259634/1‑140 (MQ=255)

                                                                               gTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGttttt                                                   >  2:1490806/1‑140 (MQ=255)

                                                                                 cAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAg                                                 <  1:2075155/140‑1 (MQ=255)

                                                                                 cAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAg                                                 <  1:791701/140‑1 (MQ=255)

                                                                                  aGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATGTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGc                                                >  1:2104328/1‑140 (MQ=255)

                                                                                   ggATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGTCCGATAAACGCCGTTACCTGGTTTTTAGCg                                               <  2:2302582/140‑1 (MQ=255)

                                                                                    gATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCg                                                                <  2:1642015/122‑1 (MQ=255)

                                                                                    gATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCg                                                                >  1:1642015/1‑122 (MQ=255)

                                                                                         tgtCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGttt                                                     <  1:762338/128‑1 (MQ=255)

                                                                                         tgtCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATcc                                         >  2:1604031/1‑140 (MQ=255)

                                                                                          gtCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCa                                        >  1:863618/1‑140 (MQ=255)

                                                                                          gtCGCCATCAAGAAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCa                                        >  2:13155/1‑140 (MQ=255)

                                                                                             gCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGt                                     <  2:2371503/140‑1 (MQ=255)

                                                                                             gCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGt                                     <  1:1027170/140‑1 (MQ=255)

                                                                                              ccATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGtt                                    >  1:1921098/1‑140 (MQ=255)

                                                                                                aTCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGa                                  >  1:2069600/1‑140 (MQ=255)

                                                                                                  cAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATg                                >  1:28185/1‑140 (MQ=255)

                                                                                                  cAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATg                                >  1:921654/1‑140 (MQ=255)

                                                                                                  cAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATg                                >  1:558277/1‑140 (MQ=255)

                                                                                                  cAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATg                                >  2:1235023/1‑140 (MQ=255)

                                                                                                  cAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATg                                >  2:874940/1‑140 (MQ=255)

                                                                                                       aGAATTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATGttttt                           <  2:2040846/140‑1 (MQ=255)

                                                                                                         aaTTTCGCCTTCCGCACGCTGCTCCGGGTCCAGTTCAAACATTTTGTTGAAGGTACGCAGCCGCGTCGATTTACCGCAGCCGGATGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATGTTTTTCa                         <  2:1968435/140‑1 (MQ=255)

                                                                                                         aaTTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATGTTTTTCa                         <  1:334542/140‑1 (MQ=255)

                                                                                                         aaTTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATGTTTTTCa                         <  1:153616/140‑1 (MQ=255)

                                                                                                         aaTTTCACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATGTTTTTCa                         <  1:13155/140‑1 (MQ=255)

                                                                                                              cACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATGTTTTTCAGGGCa                    <  2:1699671/140‑1 (MQ=255)

                                                                                                              cACCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATGTTTTTCAGGGCa                    <  1:1604031/140‑1 (MQ=255)

                                                                                                               aCCTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATGTTTTTCAGGGCAt                   >  2:2255647/1‑140 (MQ=255)

                                                                                                                ccTTCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGAt                                 >  2:2113954/1‑125 (MQ=255)

                                                                                                                   tCCGCACGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATGTTTTTCAGGGCATGGaa               >  2:836188/1‑140 (MQ=255)

                                                                                                                        aCGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATGTTTTTCAGGGCATGGAATTTGc          <  2:1738831/140‑1 (MQ=255)

                                                                                                                        aCGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATGTTTTTCAGGGCATGGAATTTGc          <  1:2180830/140‑1 (MQ=255)

                                                                                                                        aCGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATGTTTTTCAGGGCATGGAATTTGc          <  1:3811/140‑1 (MQ=255)

                                                                                                                        aCGCTGCTCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATGTTTTTCAGGGCATGGAATTTGc          <  1:205819/140‑1 (MQ=255)

                                                                                                                              ctCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATGTTTTTCAGGGCATGGAATTTGCCgtagt    >  2:1455823/1‑140 (MQ=255)

                                                                                                                              ctCCGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATGTTTTTCAGGGCATGGAATTTGCCgtagt    >  1:1957313/1‑140 (MQ=255)

                                                                                                                                ccGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATGTTTTTCAGGGCATGGAATTTGCCGtagtag  <  1:1235023/140‑1 (MQ=255)

                                                                                                                                ccGGGTGCAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATGTTTTTCAGGGCATGGAATTTGCCGtagtag  <  1:521459/140‑1 (MQ=255)

GATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCAGCGTCGATTTACCGCAGCCGGACGGCCCGATAAACGCCGTTACCTGGTTTTTAGCGATATCCAGGTTGATGTTTTTCAGGGCATGGAATTTGCCGTAGTAG  >  NC_000913/3908046‑3908313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A