Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 159 82.1 2830106 99.3% 2810295 139.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,552,783 T→C G144G (GGT→GGC)  amiA → N‑acetylmuramoyl‑L‑alanine amidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,552,7830TC97.7% 306.7 / ‑3.2 86G144G (GGT→GGC) amiAN‑acetylmuramoyl‑L‑alanine amidase A
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (47/37);  total (48/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

GGATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTG  >  NC_000913/2552647‑2552918
                                                                                                                                        |                                                                                                                                       
ggATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCt                                                                                                                                      >  1:998828/1‑140 (MQ=255)
 gATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCtt                                                                                                                                     <  2:503661/140‑1 (MQ=255)
  aTTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTc                                                                                                                                    <  1:545085/140‑1 (MQ=255)
      aTGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTa                                                                                                                                >  2:1166963/1‑140 (MQ=255)
       tGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTAt                                                                                                                               <  1:970436/140‑1 (MQ=255)
          gcgTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTg                                                                                                                            <  2:923977/140‑1 (MQ=255)
           cgTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGc                                                                                                                           >  1:1993/1‑140 (MQ=255)
            gTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGcc                                                                                                                          <  1:95106/140‑1 (MQ=255)
            gTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGcc                                                                                                                          <  1:494725/140‑1 (MQ=255)
            gTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGcc                                                                                                                          <  1:594920/140‑1 (MQ=255)
            gTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGcc                                                                                                                          <  1:279092/140‑1 (MQ=255)
            gTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGcc                                                                                                                          <  1:629816/140‑1 (MQ=255)
                       tCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCg                                                                                                               >  1:916354/1‑140 (MQ=255)
                        cTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGt                                                                                                              >  2:1119210/1‑140 (MQ=255)
                        cTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGt                                                                                                              >  2:346608/1‑140 (MQ=255)
                                     tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAgcg                                                                                                 >  2:1234678/1‑140 (MQ=255)
                                         ccACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAAt                                                                                             <  2:1082984/140‑1 (MQ=255)
                                         ccACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAAt                                                                                             >  2:854224/1‑140 (MQ=255)
                                         ccACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAAt                                                                                             <  1:981764/140‑1 (MQ=255)
                                         ccACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAAt                                                                                             <  1:714691/140‑1 (MQ=255)
                                         ccACTTTACGATCGCGTTGAAATAGCCGATAAACATGGCGCAGATTTGTTTATGTCTATTCATGCCGATGGCTTTGCCAGCCCGAAAGCTGCCGGCGCTTCGGTATGTGCCCTCTCTAACCGTGGGGCAGGTAGCGCAAt                                                                                             <  2:573074/140‑1 (MQ=255)
                                            cTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGc                                                                                          >  1:326512/1‑140 (MQ=255)
                                            cTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGc                                                                                          >  2:113646/1‑140 (MQ=255)
                                            cTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGc                                                                                          >  1:1129410/1‑140 (MQ=255)
                                             tttACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCg                                                                                         <  1:440954/140‑1 (MQ=255)
                                             tttACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCg                                                                                         >  2:365055/1‑140 (MQ=255)
                                             tttACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCg                                                                                         >  2:303825/1‑140 (MQ=255)
                                                       cgTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGt                                                                               >  2:331533/1‑140 (MQ=255)
                                                       cgTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGt                                                                               >  2:35201/1‑140 (MQ=255)
                                                       cgTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGt                                                                               >  2:1299854/1‑140 (MQ=255)
                                                       cgTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGt                                                                               >  1:1038161/1‑140 (MQ=255)
                                                       cgTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGt                                                                               >  1:712943/1‑140 (MQ=255)
                                                       cgTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGt                                                                               >  1:532879/1‑140 (MQ=255)
                                                                      tAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAAcc                                                                >  1:291123/1‑140 (MQ=255)
                                                                            tGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCg                                                          <  2:712943/140‑1 (MQ=255)
                                                                             ggCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGa                                                         >  1:625283/1‑140 (MQ=255)
                                                                             ggCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGa                                                         >  2:927805/1‑140 (MQ=255)
                                                                                    aTCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACcg                                                               <  2:1278711/127‑1 (MQ=255)
                                                                                    aTCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACcg                                                               >  1:1278711/1‑127 (MQ=255)
                                                                                        gTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGACCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCg                                              <  2:763691/140‑1 (MQ=255)
                                                                                         tttATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCgg                                             >  1:472680/1‑140 (MQ=255)
                                                                                         tttATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCgg                                             >  2:597619/1‑140 (MQ=255)
                                                                                              gTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTaaaa                                        >  1:1072317/1‑140 (MQ=255)
                                                                                              gTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTaaaa                                        >  2:631532/1‑140 (MQ=255)
                                                                                                   ttCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCg                                   >  1:636764/1‑140 (MQ=255)
                                                                                                   ttCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCg                                   >  1:1055438/1‑140 (MQ=255)
                                                                                                   ttCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCg                                   >  1:1253953/1‑140 (MQ=255)
                                                                                                   ttCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCg                                   >  1:302504/1‑140 (MQ=255)
                                                                                                   ttCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCg                                   >  1:404430/1‑140 (MQ=255)
                                                                                                   ttCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCg                                   >  2:572336/1‑140 (MQ=255)
                                                                                                   ttCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCg                                   >  2:56247/1‑140 (MQ=255)
                                                                                                    tCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGa                                  <  2:1072317/140‑1 (MQ=255)
                                                                                                    tCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGa                                  <  1:303825/140‑1 (MQ=255)
                                                                                                    tCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGa                                  <  1:365055/140‑1 (MQ=255)
                                                                                                    tCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGa                                  <  1:854224/140‑1 (MQ=255)
                                                                                                     cATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGAc                                 <  1:331533/140‑1 (MQ=255)
                                                                                                       tGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTg                               >  2:32857/1‑140 (MQ=255)
                                                                                                       tGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTg                               >  2:1311135/1‑140 (MQ=255)
                                                                                                       tGCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTg                               >  2:529475/1‑140 (MQ=255)
                                                                                                        gCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGa                              >  2:785277/1‑140 (MQ=255)
                                                                                                        gCCGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGa                              >  1:960449/1‑140 (MQ=255)
                                                                                                         ccGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGAc                             <  2:478648/140‑1 (MQ=255)
                                                                                                         ccGATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGAc                             <  1:714593/140‑1 (MQ=255)
                                                                                                           gATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACaa                           >  2:20250/1‑140 (MQ=255)
                                                                                                           gATGGCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACaa                           <  2:636764/140‑1 (MQ=255)
                                                                                                             tGGCTTTACCAACCCGAAAGATGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAgg                         >  2:934015/1‑140 (MQ=255)
                                                                                                              ggCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGa                        <  2:1038161/140‑1 (MQ=255)
                                                                                                               gCTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGAt                       <  1:56247/140‑1 (MQ=255)
                                                                                                                cTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATc                      <  1:20250/140‑1 (MQ=255)
                                                                                                                cTTTACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATc                      <  2:472680/140‑1 (MQ=255)
                                                                                                                 tttACCAACCCGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCa                     <  2:404430/140‑1 (MQ=255)
                                                                                                                         cccGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGAt                       <  1:1282853/130‑1 (MQ=255)
                                                                                                                         cccGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGAt                       >  2:1282853/1‑130 (MQ=255)
                                                                                                                         cccGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGc             >  2:729977/1‑140 (MQ=255)
                                                                                                                         cccGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGc             >  1:1100919/1‑140 (MQ=255)
                                                                                                                         cccGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGc             >  2:174162/1‑140 (MQ=255)
                                                                                                                         cccGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGc             >  1:341918/1‑140 (MQ=255)
                                                                                                                           cGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGcaa           <  1:631532/140‑1 (MQ=255)
                                                                                                                           cGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGcaa           <  2:326512/140‑1 (MQ=255)
                                                                                                                           cGAAAGCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGcaa           <  1:1119210/140‑1 (MQ=255)
                                                                                                                                gatGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGt      <  1:934015/138‑1 (MQ=255)
                                                                                                                                gCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGt      <  2:625283/140‑1 (MQ=255)
                                                                                                                                gCTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGt      <  2:294006/140‑1 (MQ=255)
                                                                                                                                 cTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTg     <  2:1993/140‑1 (MQ=255)
                                                                                                                                 cTGCCGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTg     <  2:998828/140‑1 (MQ=255)
                                                                                                                                    ccGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTg  >  2:722022/1‑140 (MQ=255)
                                                                                                                                    ccGGCGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTg  >  2:183073/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                       
GGATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCGAAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAACAAGTGCTG  >  NC_000913/2552647‑2552918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A