Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	159	82.1	2830106	99.3%	2810295	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,616,067	T→C	V276A (GTG→GCG)	nikB →	Ni(2(+)) ABC transporter membrane subunit NikB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,616,067	0	T	C	100.0%	182.4 / NA	51	V276A (GTG→GCG)	nikB	Ni(2(+)) ABC transporter membrane subunit NikB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/27); total (24/27)

GCTGCCGATGATCACCGCCGTGGGGATGCATATCGGCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGTGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCT  >  NC_000913/3615933‑3616197

gCTGCCGATGATCACCGCCGTGGGGATGCATATCGGCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATcc                                                                                                                               >  2:1372363/1‑140 (MQ=255)

 cTGCCGATGATCACCGCCGTGGGGATGCATATCGGCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCa                                                                                                                              <  2:1298967/140‑1 (MQ=255)

 cTGCCGATGATCACCGCCGTGGGGATGCATATCGGCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCa                                                                                                                              <  1:300475/140‑1 (MQ=255)

      gatgatCACCGCCGTGGGGATGCATATCGGCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCt                                                                                                                         <  2:1261071/140‑1 (MQ=255)

          atCACCGCCGTGGGGATGCATATCGGCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTAc                                                                                                                     <  1:630128/140‑1 (MQ=255)

              ccgccgTGGGGATGCATATCGGCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCtg                                                                                                                 >  1:936659/1‑140 (MQ=255)

              ccgccgTGGGGATGCATATCGGCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCtg                                                                                                                 >  2:415137/1‑140 (MQ=255)

              ccgccgTGGGGATGCATATCGGCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCtg                                                                                                                 >  1:512657/1‑140 (MQ=255)

              ccgccgT‑GGGATGCATATCGGCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCtga                                                                                                                >  1:553338/1‑140 (MQ=255)

                gcagTGGGGATGAATATCGGCGGTCTGATTGGCGGGCCGATGATTATCGAAAATATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCtgat                                                                                                               <  2:934460/137‑1 (MQ=255)

                  ccgtgggATGCATATCGGCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCtgatga                                                                                                             <  2:553338/136‑1 (MQ=255)

                      gggATGCATATCGGCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGAtggt                                                                                                         <  1:1116112/140‑1 (MQ=255)

                         aTGCATATCGGCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGTGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGAtggtggg                                                                                                      >  1:605103/1‑139 (MQ=255)

                         aTGCATATCGGCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGAtggtggt                                                                                                      <  1:543149/140‑1 (MQ=255)

                           gCATATCGGCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGAtggtggtgg                                                                                                    >  2:1116634/1‑140 (MQ=255)

                                tCGGCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGttttt                                                                                               >  1:34512/1‑140 (MQ=255)

                                  ggCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTtgt                                                                                             <  2:34512/140‑1 (MQ=255)

                                     gAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGt                                                                                          <  1:1372363/140‑1 (MQ=255)

                                      aaCTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTc                                                                                         >  2:1093349/1‑140 (MQ=255)

                                      aaCTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTc                                                                                         >  1:250494/1‑140 (MQ=255)

                                      aaCTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTc                                                                                         <  2:438539/140‑1 (MQ=255)

                                      aaCTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTc                                                                                         <  2:1107121/140‑1 (MQ=255)

                                      aaCTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGGGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGGGGGGGGTTTTGTGGTc                                                                                         >  2:406187/1‑140 (MQ=255)

                                       aCTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGttttt                                                                                               >  2:917908/1‑133 (MQ=255)

                                       aCTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGttttt                                                                                               <  1:917908/133‑1 (MQ=255)

                                           aTTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTaa                                                                                    >  1:65440/1‑140 (MQ=255)

                                                cGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGa                                                                               >  1:1295057/1‑140 (MQ=255)

                                                 gggACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGAt                                                                              <  1:1093349/140‑1 (MQ=255)

                                                     cGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGGGCTATGCGGGGTCGGCGATTTTTTACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGGTTTTGTGGGCTGTAATTTGATTGTc                                                                          >  2:352954/1‑140 (MQ=255)

                                                        tgatTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGAt                                                                       <  1:1116634/140‑1 (MQ=255)

                                                              tCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTc                                                                 >  1:1065348/1‑140 (MQ=255)

                                                                   aaCATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAAcgc                                                            <  1:1084703/140‑1 (MQ=255)

                                                                   aaCATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAAcgc                                                            <  2:1065348/140‑1 (MQ=255)

                                                                    aCATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAAcgcc                                                           >  2:791397/1‑140 (MQ=255)

                                                                           tGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTgg                                                    >  1:883867/1‑140 (MQ=255)

                                                                             ccTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGAc                                                  <  2:512657/140‑1 (MQ=255)

                                                                                     ggCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCAt                                          >  1:421586/1‑140 (MQ=255)

                                                                                       cGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATtc                                        >  1:1369676/1‑140 (MQ=255)

                                                                                         tCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATtcgt                                      <  2:250494/140‑1 (MQ=255)

                                                                                         tCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATtcgt                                      <  2:361708/140‑1 (MQ=255)

                                                                                                cTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATg                               >  1:131944/1‑140 (MQ=255)

                                                                                                 tATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGa                              <  1:415137/140‑1 (MQ=255)

                                                                                                   tGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAg                            <  2:927095/140‑1 (MQ=255)

                                                                                                          ttggCGATTTTAAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCa                     >  1:748314/3‑140 (MQ=255)

                                                                                                                  ttttAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGaa             <  2:267269/140‑1 (MQ=255)

                                                                                                                  ttttAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGaa             <  1:683108/140‑1 (MQ=255)

                                                                                                                   tttAACCGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAAc            <  1:258914/140‑1 (MQ=255)

                                                                                                                        ccGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACttttt       >  1:169794/1‑140 (MQ=255)

                                                                                                                         cGTGACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTc      <  2:131944/140‑1 (MQ=255)

                                                                                                                            gACTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCctc   >  1:1389609/1‑140 (MQ=255)

                                                                                                                             aCTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCctct  <  2:65440/140‑1 (MQ=255)

                                                                                                                             aCTATCCGGCGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCctct  >  2:965604/1‑140 (MQ=255)

GCTGCCGATGATCACCGCCGTGGGGATGCATATCGGCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGTGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAACTTTTTCCTCT  >  NC_000913/3615933‑3616197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A