Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	159	82.1	2830106	99.3%	2810295	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,322,037	A→G	V129A (GTC→GCC)	phnF ←	putative transcriptional regulator PhnF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,322,037	0	A	G	100.0%	229.6 / NA	62	V129A (GTC→GCC)	phnF	putative transcriptional regulator PhnF
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/31); total (31/31)

TGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGA  >  NC_000913/4321902‑4322172

tGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGtt                                                                                                                                     <  2:1313639/140‑1 (MQ=255)

  cTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTtct                                                                                                                                   >  2:978427/1‑140 (MQ=255)

  cTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTtct                                                                                                                                   >  2:972065/1‑140 (MQ=255)

  cTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTtct                                                                                                                                   >  2:788411/1‑140 (MQ=255)

   tCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTtctc                                                                                                                                  <  1:1031708/140‑1 (MQ=255)

   tCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTtctc                                                                                                                                  <  1:768720/140‑1 (MQ=255)

      cgcAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTcccc                                                                                                                               <  1:1113729/140‑1 (MQ=255)

            aaaTCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGt                                                                                                                         <  1:1393020/140‑1 (MQ=255)

            aaaTCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGt                                                                                                                         <  2:1099900/140‑1 (MQ=255)

            aaaTCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGt                                                                                                                         <  1:1152534/140‑1 (MQ=255)

               tCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAAt                                                                                                                      <  1:944233/140‑1 (MQ=255)

                   gCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATcccc                                                                                                                  <  1:454267/140‑1 (MQ=255)

                   gCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATcccc                                                                                                                  >  2:943726/1‑140 (MQ=255)

                        gAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGc                                                                                                             <  1:978427/140‑1 (MQ=255)

                         aGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCg                                                                                                            >  1:1308430/1‑140 (MQ=255)

                          gCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGt                                                                                                           <  1:943726/140‑1 (MQ=255)

                                      gCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGc                                                                                               <  2:775855/140‑1 (MQ=255)

                                            cAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGAcg                                                                                         <  1:1182437/140‑1 (MQ=255)

                                            cAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGAcg                                                                                         <  2:181706/140‑1 (MQ=255)

                                               cGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCgg                                                                                      >  2:1236059/1‑140 (MQ=255)

                                               cGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCgg                                                                                      >  1:950079/1‑140 (MQ=255)

                                                gTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCggg                                                                                     <  2:816214/140‑1 (MQ=255)

                                                   ggCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGgcg                                                                                  <  2:176610/140‑1 (MQ=255)

                                                               aGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAg                                                                      >  1:1221898/1‑140 (MQ=255)

                                                               aGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAg                                                                      <  2:1308430/140‑1 (MQ=255)

                                                               aGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAg                                                                      <  1:410365/140‑1 (MQ=255)

                                                                ggTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGc                                                                     >  1:331190/1‑140 (MQ=255)

                                                                   ccGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGGGGCCGGACGCGGGGCGCAATACCGAAAGCAGt                                                                  >  2:1248970/1‑140 (MQ=255)

                                                                               ggTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCtggtg                                                      >  1:784680/1‑140 (MQ=255)

                                                                                     ttAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATgg                                                <  2:331190/140‑1 (MQ=255)

                                                                                     ttAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATgg                                                >  1:100726/1‑140 (MQ=255)

                                                                                      tAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGc                                               <  2:1221898/140‑1 (MQ=255)

                                                                                           agagCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGccc                                          <  1:1236059/140‑1 (MQ=255)

                                                                                           agagCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGccc                                          <  2:485793/140‑1 (MQ=255)

                                                                                             agCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGccc                                          <  1:351301/138‑1 (MQ=255)

                                                                                             agCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGccc                                          >  2:351301/1‑138 (MQ=255)

                                                                                              gcgcGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGa                                       >  2:433138/1‑140 (MQ=255)

                                                                                               cgcgACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGAt                                      >  2:119040/1‑140 (MQ=255)

                                                                                               cgcgACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGAt                                      >  2:213899/1‑140 (MQ=255)

                                                                                               cgcgACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGAt                                      >  2:331839/1‑140 (MQ=255)

                                                                                               cgcgACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGAt                                      >  2:789488/1‑140 (MQ=255)

                                                                                               cgcgACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGAt                                      >  2:486319/1‑140 (MQ=255)

                                                                                               cgcgACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGAt                                      <  2:784680/140‑1 (MQ=255)

                                                                                               cacgACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGAt                                      >  2:896726/3‑140 (MQ=255)

                                                                                                  gACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCca                                   >  2:776376/1‑140 (MQ=255)

                                                                                                          tGACACGACGCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTc                           <  1:538696/140‑1 (MQ=255)

                                                                                                              acgacgCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGc                       >  1:907800/1‑140 (MQ=255)

                                                                                                              acgacgCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGc                       <  1:119040/140‑1 (MQ=255)

                                                                                                                   gCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTaaaa                  >  2:761544/1‑140 (MQ=255)

                                                                                                                   gCAGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTaaaa                  <  2:97333/140‑1 (MQ=255)

                                                                                                                     aGGGTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAAcg                <  1:776376/140‑1 (MQ=255)

                                                                                                                       ggTGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAAcgcg              >  1:825531/1‑140 (MQ=255)

                                                                                                                         tGCGCAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGcc            <  1:786606/140‑1 (MQ=255)

                                                                                                                            gcAGGTGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTgg         >  1:963755/1‑140 (MQ=255)

                                                                                                                                 tGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGtt    >  1:659744/1‑140 (MQ=255)

                                                                                                                                 tGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGtt    >  1:480152/1‑140 (MQ=255)

                                                                                                                                 tGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGtt    >  1:473167/1‑140 (MQ=255)

                                                                                                                                 tGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGtt    >  2:976805/1‑140 (MQ=255)

                                                                                                                                 tGGATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGGGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGtt    >  2:129543/1‑140 (MQ=255)

                                                                                                                                   gATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGa  <  2:473167/140‑1 (MQ=255)

                                                                                                                                   gATGGCGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGa  <  2:907800/140‑1 (MQ=255)

                                                                                                                                   gATGGCGCTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGa  >  2:1175971/1‑140 (MQ=255)

TGCTCGCGCAGAAAATCGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCGAAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGA  >  NC_000913/4321902‑4322172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A