Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	93,243	A→G	T26T (ACA→ACG)	murE →	UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	93,243	0	A	G	100.0%	169.0 / NA	48	T26T (ACA→ACG)	murE	UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/24); total (24/24)

AACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGC  >  NC_000913/93119‑93377

aaCGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGa                                                                                                                                   >  1:1080341/1‑130 (MQ=255)

aaCGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGa                                                                                                                                   <  2:1080341/130‑1 (MQ=255)

     gCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGa                                                                                                                                   >  2:724275/1‑125 (MQ=255)

     gCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGa                                                                                                                                   <  1:724275/125‑1 (MQ=255)

           aaaaTGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCggcggg                                                                                                              <  2:1036822/140‑1 (MQ=255)

           aaaaTGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCggcggg                                                                                                              <  2:253176/140‑1 (MQ=255)

                     tgtgATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCttt                                                                                                    <  1:52090/140‑1 (MQ=255)

                        gATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTa                                                                                                 <  1:420923/140‑1 (MQ=255)

                         aTTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAg                                                                                                <  2:736435/140‑1 (MQ=255)

                         aTTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAg                                                                                                <  1:635523/140‑1 (MQ=255)

                             aTCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTgt                                                                                            <  1:329147/140‑1 (MQ=255)

                                   gCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAgg                                                                                      >  2:932228/1‑140 (MQ=255)

                                    cGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGt                                                                                     <  2:913028/140‑1 (MQ=255)

                                        gggACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGtcatc                                                                                 >  2:400626/1‑140 (MQ=255)

                                        gggACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGtcatc                                                                                 >  1:912417/1‑140 (MQ=255)

                                            cAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGc                                                                             >  1:373386/1‑140 (MQ=255)

                                             aGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCg                                                                            >  1:220335/1‑140 (MQ=255)

                                             aGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCg                                                                            >  1:144014/1‑140 (MQ=255)

                                               gTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCGCCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGa                                                                          <  2:144014/140‑1 (MQ=255)

                                               gTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGa                                                                          <  2:220335/140‑1 (MQ=255)

                                                tGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGAc                                                                         <  1:523577/140‑1 (MQ=255)

                                                   cAGATCGTAATTTGCGCGACCTTCTTGCTTCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACggg                                                                      <  1:264259/140‑1 (MQ=255)

                                                   cAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGGTGTCTTTGTAGGTGGAGTAGGTCCTCAGGCGGACggg                                                                      >  1:450053/1‑140 (MQ=255)

                                                   cAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACggg                                                                      <  2:743337/140‑1 (MQ=255)

                                                     gATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCg                                                                    >  1:484438/1‑140 (MQ=255)

                                                         gTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCg                                                                    <  1:783515/136‑1 (MQ=255)

                                                         gTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCg                                                                    >  2:783515/1‑136 (MQ=255)

                                                           aaTTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGata                                                              <  1:170266/140‑1 (MQ=255)

                                                                 cgcgACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATccc                                                        >  1:163249/1‑140 (MQ=255)

                                                                 cgcgACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATccc                                                        >  1:127240/1‑140 (MQ=255)

                                                                    gACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCa                                                     <  2:127240/140‑1 (MQ=255)

                                                                                ccGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCa                                         >  2:353593/1‑140 (MQ=255)

                                                                                   tGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGGTCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCCAgg                                      >  1:358011/1‑140 (MQ=255)

                                                                                   tGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGGCGGGGGTCGATATATCCCGCAGGCGATAGCGCAAgg                                      >  2:937294/1‑140 (MQ=255)

                                                                                   tGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGGGTCGATATATCCCGCAGGCGATAGCGCAAgg                                      >  2:848495/1‑140 (MQ=255)

                                                                                   tGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCg                                                       <  2:201936/123‑1 (MQ=255)

                                                                                   tGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCg                                                       >  1:201936/1‑123 (MQ=255)

                                                                                        gCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTgg                                 >  2:458557/1‑140 (MQ=255)

                                                                                          cAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCt                               <  2:555819/140‑1 (MQ=255)

                                                                                          cAGACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCt                               <  2:163249/140‑1 (MQ=255)

                                                                                            gACGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGTGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGc                             <  1:1091735/140‑1 (MQ=255)

                                                                                             aCGCACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGcc                            >  1:1070765/1‑140 (MQ=255)

                                                                                                cACCTTCGCGAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGGGTGGCTGCCatt                         >  2:320028/1‑140 (MQ=255)

                                                                                                        cgAGCACTGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGGCGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGGGTGGCTGCCATTATTGCaga                 >  2:488394/1‑140 (MQ=255)

                                                                                                               tGCGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGaaa          >  2:40836/1‑140 (MQ=255)

                                                                                                                 cGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGa        >  1:51677/1‑140 (MQ=255)

                                                                                                                 cGAGAGATGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGa        <  1:472693/140‑1 (MQ=255)

                                                                                                                     agaTGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAg    <  2:373386/140‑1 (MQ=255)

                                                                                                                       aTGACGCTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGc  <  2:450053/140‑1 (MQ=255)

AACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGC  >  NC_000913/93119‑93377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A