Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F28 I1 R1 180 66.5 2263572 99.4% 2249990 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,422,316 A→G T112A (ACC→GCC)  ydaV → Rac prophage; putative ATP‑binding protein YdaV

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,422,3160AG100.0% 180.8 / NA 51T112A (ACC→GCC) ydaVRac prophage; putative ATP‑binding protein YdaV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/26);  total (25/26)

ATTGCACCGCAAATGCTCGTTTTCGAATTACCAGGTGCAGAACGAAGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCA  >  NC_000913/1422180‑1422437
                                                                                                                                        |                                                                                                                         
aTTGCACCGCAAATGCTCGTTTTCGAATTACCAGGTGCAGAACGAAGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCg                                                                                                                        <  1:761654/140‑1 (MQ=255)
aTTGCACCGCAAATGCTCGTTTTCGAATTACCAGGTGCAGAACGAAGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCg                                                                                                                        <  2:492351/140‑1 (MQ=255)
  tGCACCGCAAATGCTCGTTTTCGAATTACCAGGTGCAAAACGAAGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCggg                                                                                                                      <  2:377223/140‑1 (MQ=255)
       cGCAAATGCTCGTTTTCGAATTACCAGGTGCAGAACGAAGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGgaagaa                                                                                                                 >  2:576616/1‑140 (MQ=255)
        gCAAATGCTCGTTTTCGAATTACCAGGTGCAGAACGAAGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCggg                                                                                                                      >  1:921772/1‑134 (MQ=255)
        gCAAATGCTCGTTTTCGAATTACCAGGTGCAGAACGAAGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCggg                                                                                                                      <  2:921772/134‑1 (MQ=255)
            aTGCTCGTTTTCGAATTACCAGGTGCAGAACGAAGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACt                                                                                                            >  2:33931/1‑140 (MQ=255)
                tCGTTTTCGAATTACCAGGTGCAGAACGAAGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGc                                                                                                        <  1:243362/140‑1 (MQ=255)
                   ttttCGAATTACCAGGTGCAGAACGAAGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGc                                                                                                     <  2:236494/140‑1 (MQ=255)
                     ttCGAATTACCAGGTGCAGAACGAAGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAg                                                                                                   <  2:1089411/140‑1 (MQ=255)
                         aaTTACCAGGTGCAGAACGAAGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTAt                                                                                               <  1:9057/140‑1 (MQ=255)
                              ccAGGTGCAGAACGAAGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGa                                                                                          >  1:685814/1‑140 (MQ=255)
                                    gCAGAACGAAGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGcc                                                                                    <  1:353685/140‑1 (MQ=255)
                                     cAGAACGAAGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCct                                                                                   >  2:869799/1‑140 (MQ=255)
                                      aGAACGAAGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCctg                                                                                  >  2:1051247/1‑140 (MQ=255)
                                          cGAAGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGa                                                                              >  2:495395/1‑140 (MQ=255)
                                            aaGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGaaa                                                                            >  2:82248/1‑140 (MQ=255)
                                            aaGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGaaa                                                                            <  1:1063535/140‑1 (MQ=255)
                                             aGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAg                                                                           >  2:675819/1‑140 (MQ=255)
                                              gggCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGa                                                                          <  2:493324/140‑1 (MQ=255)
                                                  aGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGt                                                                      <  1:82248/140‑1 (MQ=255)
                                                  aGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGt                                                                      <  1:1051247/140‑1 (MQ=255)
                                                  aGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGt                                                                      <  1:675819/140‑1 (MQ=255)
                                                        aCGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACa                                                                <  2:33137/140‑1 (MQ=255)
                                                         cgcgTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAg                                                               <  1:850623/140‑1 (MQ=255)
                                                         cgcgTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAg                                                               >  2:147665/1‑140 (MQ=255)
                                                              tGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGAtt                                                          >  1:205293/1‑140 (MQ=255)
                                                                          aaTCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTg                                              >  2:1013423/1‑140 (MQ=255)
                                                                           aTCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTgg                                             <  1:576616/140‑1 (MQ=255)
                                                                           aTCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTgg                                             <  2:205293/140‑1 (MQ=255)
                                                                             ccATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCt                                           <  1:1022245/140‑1 (MQ=255)
                                                                                tCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGt                                               <  1:455401/133‑1 (MQ=255)
                                                                                tCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGt                                               >  2:455401/1‑133 (MQ=255)
                                                                                              atgaCCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCt                          >  2:822264/1‑140 (MQ=255)
                                                                                              atgaCCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCt                          >  1:283397/1‑140 (MQ=255)
                                                                                              atgaCCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCt                          >  1:1088991/1‑140 (MQ=255)
                                                                                                gaCCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGc                        >  1:372919/1‑140 (MQ=255)
                                                                                                  ccGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCAc                      <  1:1013423/140‑1 (MQ=255)
                                                                                                     ggTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGc                    <  2:944208/139‑1 (MQ=255)
                                                                                                     ggTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGc                    >  1:944208/1‑139 (MQ=255)
                                                                                                      gtgtAGAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGc                    >  2:709692/1‑138 (MQ=255)
                                                                                                      gtgtAGAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGc                    <  1:709692/138‑1 (MQ=255)
                                                                                                         tACAAATTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCt               >  1:105008/1‑140 (MQ=255)
                                                                                                            aaaTTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATg            <  2:1088991/140‑1 (MQ=255)
                                                                                                              aTTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGAc          <  2:105008/140‑1 (MQ=255)
                                                                                                              aTTTTGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGAc          >  1:869230/1‑140 (MQ=255)
                                                                                                               ttttGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACg         >  2:27838/1‑140 (MQ=255)
                                                                                                               ttttGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACg         <  2:682877/140‑1 (MQ=255)
                                                                                                                tttGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGa        >  1:301695/1‑140 (MQ=255)
                                                                                                                tttGCGTTCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGa        >  1:152143/1‑140 (MQ=255)
                                                                                                                      ttCAGCGGAAAACCTGGTGCCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCa  >  1:219766/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                         
ATTGCACCGCAAATGCTCGTTTTCGAATTACCAGGTGCAGAACGAAGGGCAGCGATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCA  >  NC_000913/1422180‑1422437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A