Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,446,045	G→A	D56N (GAC→AAC)	ydbL →	DUF1318 domain‑containing protein YdbL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,446,045	0	G	A	100.0%	178.3 / NA	52	D56N (GAC→AAC)	ydbL	DUF1318 domain‑containing protein YdbL
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/31); total (21/31)

CTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCTCGGGCCAAACCGGGGGAATATGTACAAGGGATAAA  >  NC_000913/1445910‑1446181

cTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACAt                                                                                                                                      <  2:886133/140‑1 (MQ=255)

      gggCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGc                                                                                                                                <  1:668282/140‑1 (MQ=255)

       ggCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGcc                                                                                                                               <  1:533648/140‑1 (MQ=255)

        gCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCg                                                                                                                              >  1:950580/1‑140 (MQ=255)

                  agcaATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGc                                                                                                                    <  1:891829/140‑1 (MQ=255)

                     aaTGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAg                                                                                                                 <  2:950580/140‑1 (MQ=255)

                     aaTGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAg                                                                                                                 <  1:1119066/140‑1 (MQ=255)

                      aTGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGt                                                                                                                >  2:807365/1‑140 (MQ=255)

                      aTGTAATGACATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGt                                                                                                                >  2:575557/1‑140 (MQ=255)

                           aTGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGGTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACca                                                                                                           >  1:393799/1‑140 (MQ=255)

                                  tGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCGACAACTg                                                                                                    >  2:373653/1‑140 (MQ=255)

                                     ctctGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCGACAACTGgca                                                                                                 <  1:373653/140‑1 (MQ=255)

                                     ctctGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGgca                                                                                                 >  2:650622/1‑140 (MQ=255)

                                      tctGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGgcaa                                                                                                <  1:575557/140‑1 (MQ=255)

                                          gATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGgcaaagca                                                                                            <  2:685666/140‑1 (MQ=255)

                                           aTGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGgcaaagcaa                                                                                           <  2:271251/140‑1 (MQ=255)

                                              aaGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAaat                                                                                        >  2:762933/1‑140 (MQ=255)

                                                gCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGGTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAaataa                                                                                      >  2:776053/1‑140 (MQ=255)

                                                     aaCTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATgtgt                                                                                 <  1:297891/140‑1 (MQ=255)

                                                     aaCTCAGGGGCGGGGAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATgtgt                                                                                 >  1:257822/1‑140 (MQ=255)

                                                       cTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCg                                                                               <  1:842263/140‑1 (MQ=255)

                                                          aGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTa                                                                            >  2:171171/1‑140 (MQ=255)

                                                            gggCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAga                                                                          <  1:762933/140‑1 (MQ=255)

                                                                 ggTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGata                                                                     >  1:929506/1‑140 (MQ=255)

                                                                      gTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAgatga                                                                       <  1:81573/133‑1 (MQ=255)

                                                                      gTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAgatga                                                                       >  2:81573/1‑133 (MQ=255)

                                                                      gTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATcgcg                                                                >  1:882834/1‑140 (MQ=255)

                                                                             aTTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCg                                                         >  2:106373/1‑140 (MQ=255)

                                                                                        aTCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGc                                                        >  2:439323/1‑130 (MQ=255)

                                                                                        aTCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGc                                                        <  1:439323/130‑1 (MQ=255)

                                                                                        aTCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAg                                              >  2:583597/1‑140 (MQ=255)

                                                                                            ggTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCg                                          <  1:171171/140‑1 (MQ=255)

                                                                                             gTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGt                                         >  1:564445/1‑140 (MQ=255)

                                                                                                gcgcTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGc                                      >  2:1114886/1‑140 (MQ=255)

                                                                                                 cgcTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCt                                     <  1:583597/140‑1 (MQ=255)

                                                                                                 cgcTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCt                                     <  2:548144/140‑1 (MQ=255)

                                                                                                 cgcTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCt                                     <  1:776053/140‑1 (MQ=255)

                                                                                                      aaaaCGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCTCGGGc                                <  1:106373/140‑1 (MQ=255)

                                                                                                      aaaaCGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCTCGGGc                                <  2:302927/140‑1 (MQ=255)

                                                                                                        aaCGGATGCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCTCGGGCCa                              <  2:564445/140‑1 (MQ=255)

                                                                                                               gCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCTCGGGCCAAACCggg                       <  2:28164/140‑1 (MQ=255)

                                                                                                               gCTGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCTCGGGCCAAACCggg                       <  1:863706/140‑1 (MQ=255)

                                                                                                                 tGAAACAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCTCGGGCCAAACCggggg                     <  1:578285/140‑1 (MQ=255)

                                                                                                                     aCAGAGAAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCTCGGGCCAAACCGGGGGAata                 >  1:231031/1‑140 (MQ=255)

                                                                                                                         agaAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCTCGGGCCAAACCGGGGGAATATGTa             >  2:120529/1‑140 (MQ=255)

                                                                                                                         agaAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCTCGGGCCAAACCGGGGGAATATGTa             >  2:11782/1‑140 (MQ=255)

                                                                                                                          gaAATTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCTCGGGCTAAACCGGGGGAATATGTAc            <  2:929506/140‑1 (MQ=255)

                                                                                                                            aaTTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCTCGGGCCAAACCGGGGGAATATGTACaa          <  2:710940/140‑1 (MQ=255)

                                                                                                                             aTTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCTCGGGCCAAACCGGGGGa                    <  2:218547/129‑1 (MQ=255)

                                                                                                                             aTTAGTAGCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCTCGGGCCAAACCGGGGGa                    >  1:218547/1‑129 (MQ=255)

                                                                                                                                    gCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCTCGGGCCAAACCGGGGGAATATGTACAAGGGATaaa  <  2:393799/140‑1 (MQ=255)

                                                                                                                                    gCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCTCGGGCCAAACCGGGGGAATATGTACAAGGGATaaa  <  1:11782/140‑1 (MQ=255)

                                                                                                                                    gCCAACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCTCGGGCCAAACCGGGGGAATATGTACAAGGGATaaa  <  1:650622/140‑1 (MQ=255)

CTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCGAAACTCGCCGGGCAAAAGCTCGTAGCTCGGGCCAAACCGGGGGAATATGTACAAGGGATAAA  >  NC_000913/1445910‑1446181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A