Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F28 I1 R1 180 66.5 2263572 99.4% 2249990 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,628,250 T→C V245A (GTC→GCC)  ydfG → 3‑hydroxy acid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,628,2500TC100.0% 177.2 / NA 51V245A (GTC→GCC) ydfG3‑hydroxy acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/25);  total (26/25)

CTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTAATCCCACACAGC  >  NC_000913/1628116‑1628385
                                                                                                                                      |                                                                                                                                       
ttATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCAcc                                                                                                                                    >  2:629313/2‑140 (MQ=255)
             gTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTaa                                                                                                                            >  1:547317/1‑135 (MQ=255)
             gTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTaa                                                                                                                            <  2:547317/135‑1 (MQ=255)
             gTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAAttttt                                                                                                                       >  1:397638/1‑140 (MQ=255)
              ttGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTta                                                                                                                      >  2:613000/1‑140 (MQ=255)
                 cATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATAc                                                                                                                   <  1:1031654/140‑1 (MQ=255)
                 cATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATAc                                                                                                                   <  1:613000/140‑1 (MQ=255)
                    tGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCg                                                                                                                >  1:47342/1‑140 (MQ=255)
                      aCGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGc                                                                                                              >  1:295539/1‑140 (MQ=255)
                        gCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGt                                                                                                            <  1:939586/140‑1 (MQ=255)
                             aagaTGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTg                                                                                                       <  2:595235/140‑1 (MQ=255)
                              agaTGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGc                                                                                                      <  1:386431/140‑1 (MQ=255)
                              agaTGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGc                                                                                                      <  1:258580/140‑1 (MQ=255)
                              agaTGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGc                                                                                                      <  2:797136/140‑1 (MQ=255)
                                       cGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTAtt                                                                                             >  2:620579/1‑140 (MQ=255)
                                       cGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTAtt                                                                                             >  1:253683/1‑140 (MQ=255)
                                          aGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCt                                                                                          >  2:661230/1‑140 (MQ=255)
                                             cGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGt                                                                                       <  1:905956/140‑1 (MQ=255)
                                                    tgggtgTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACaaaa                                                                                <  2:85121/140‑1 (MQ=255)
                                                        tgtCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCt                                                                                          <  1:53736/126‑1 (MQ=255)
                                                        tgtCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCt                                                                                          >  2:53736/1‑126 (MQ=255)
                                                                 tgcctgcTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTc                                                                   <  2:1055619/140‑1 (MQ=255)
                                                                 tgcctgcTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTc                                                                   >  2:649505/1‑140 (MQ=255)
                                                                     tgcTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGc                                                               <  2:47342/140‑1 (MQ=255)
                                                                            gTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAac                                                        >  1:238417/1‑140 (MQ=255)
                                                                                   tcaataCCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAActcactca                                                 <  1:620579/140‑1 (MQ=255)
                                                                                       taCCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCAcaag                                             <  2:238417/140‑1 (MQ=255)
                                                                                         cccTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTcaca                                               <  2:672993/136‑1 (MQ=255)
                                                                                         cccTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTcaca                                               >  1:672993/1‑136 (MQ=255)
                                                                                          ccTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCAcaagcaa                                          >  2:659230/1‑140 (MQ=255)
                                                                                          ccTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCCTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCAcaagcaa                                          <  2:253683/140‑1 (MQ=255)
                                                                                          ccTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAAGGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCCGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCAcaagcaa                                          >  2:1123653/1‑140 (MQ=255)
                                                                                            tGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGa                                        >  1:526036/1‑140 (MQ=255)
                                                                                             ggAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGaa                                       >  1:557703/1‑140 (MQ=255)
                                                                                                      gCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAAt                                  >  2:880169/1‑136 (MQ=255)
                                                                                                      gCCGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAAt                                  <  1:880169/136‑1 (MQ=255)
                                                                                                        cGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGt                            >  2:247122/1‑140 (MQ=255)
                                                                                                        cGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGt                            >  1:964671/1‑140 (MQ=255)
                                                                                                        cGGTTACCCAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGt                            >  2:865989/1‑140 (MQ=255)
                                                                                                               ccAAAGCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACg                     <  2:964671/140‑1 (MQ=255)
                                                                                                                    gCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGACCGAATGACCGTCGAAACGCAACt                <  2:798219/140‑1 (MQ=255)
                                                                                                                    gCTATGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACt                <  2:295539/140‑1 (MQ=255)
                                                                                                                       aTGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTaa             <  1:247122/140‑1 (MQ=255)
                                                                                                                        tGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTAAt            >  2:514580/1‑140 (MQ=255)
                                                                                                                        tGCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTAAt            >  2:175989/1‑140 (MQ=255)
                                                                                                                         gCCGGACTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTAATc           >  1:424460/1‑140 (MQ=255)
                                                                                                                              aCTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTAATCCcaca      <  2:397638/140‑1 (MQ=255)
                                                                                                                              aCTGAATGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTAATCCcaca      <  1:661230/140‑1 (MQ=255)
                                                                                                                                  aaTGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTAAt            >  2:362940/1‑130 (MQ=255)
                                                                                                                                  aaTGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTAAt            <  1:362940/130‑1 (MQ=255)
                                                                                                                                  aaTGCCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTAATCCCACACAGc  >  2:95248/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                       
CTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTAATCCCACACAGC  >  NC_000913/1628116‑1628385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A