Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F28 I1 R1 180 66.5 2263572 99.4% 2249990 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,951,982 G→A P196P (CCG→CCA)  yecE → DUF72 domain‑containing protein YecE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,951,9820GA100.0% 207.1 / NA 59P196P (CCG→CCA) yecEDUF72 domain‑containing protein YecE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/27);  total (32/27)

CAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAGACATTGCCCAGGCAC  >  NC_000913/1951848‑1952118
                                                                                                                                      |                                                                                                                                        
cAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGAt                                                                                                                                     >  2:146436/1‑140 (MQ=255)
cAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGAt                                                                                                                                     <  2:538876/140‑1 (MQ=255)
   cgcgGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCg                                                                                                                                  >  1:551880/1‑140 (MQ=255)
   cgcgGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCg                                                                                                                                  >  1:402888/1‑140 (MQ=255)
   cgcgGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCg                                                                                                                                  >  2:475156/1‑140 (MQ=255)
   cgcgGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCg                                                                                                                                  >  2:102214/1‑140 (MQ=255)
       gcgTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGtttt                                                                                                                              >  1:365699/1‑140 (MQ=255)
        cgTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCT‑AAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTAt                                                                                                                            <  2:803660/140‑1 (MQ=255)
         gTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTAt                                                                                                                            <  1:102214/140‑1 (MQ=255)
            aaTCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCgg                                                                                                                         <  2:665082/140‑1 (MQ=255)
            aaTCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCgg                                                                                                                         <  2:792829/140‑1 (MQ=255)
            aaTCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCgg                                                                                                                         <  2:872744/140‑1 (MQ=255)
            aaTCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCgg                                                                                                                         <  2:200736/140‑1 (MQ=255)
            aaTCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCgg                                                                                                                         <  2:365699/140‑1 (MQ=255)
             aTCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGt                                                                                                                        <  2:804910/140‑1 (MQ=255)
             aTCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGt                                                                                                                        <  2:402888/140‑1 (MQ=255)
                gggTGATTTTAGACAGCCGCCCGGTCCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGAt                                                                                                                                     <  2:987677/124‑1 (MQ=255)
                gggTGATTTTAGACAGCCGCCCGGTCCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGAt                                                                                                                                     >  1:987677/1‑124 (MQ=255)
                  gTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTAt                                                                                                                            <  2:482218/131‑1 (MQ=255)
                  gTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTAt                                                                                                                            >  1:482218/1‑131 (MQ=255)
                  gTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGtga                                                                                                                   >  1:534385/1‑140 (MQ=255)
                                ccgccCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCaaa                                                                                                     >  1:158622/1‑140 (MQ=255)
                                ccgccCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCaaa                                                                                                     >  1:642864/1‑140 (MQ=255)
                                 cgccCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCaaaa                                                                                                    >  2:813378/1‑140 (MQ=255)
                                   cccGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAATGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTCATCCGTTTTATCGGAAGTGATGATATGACGCAAAAcc                                                                                                  <  2:421908/140‑1 (MQ=255)
                                                         cacaGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGtt                                                                            >  1:1086273/1‑140 (MQ=255)
                                                              tGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACaaa                                                                       >  1:874712/1‑140 (MQ=255)
                                                              tGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACaaa                                                                       >  1:285544/1‑140 (MQ=255)
                                                              tGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACaaa                                                                       >  1:190040/1‑140 (MQ=255)
                                                                 aGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAAt                                                                    <  2:1086273/140‑1 (MQ=255)
                                                                 aGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAAt                                                                    <  2:158622/140‑1 (MQ=255)
                                                                     aTTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAgc                                                                <  1:146436/140‑1 (MQ=255)
                                                                      ttCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAg                                                                 >  1:1006448/1‑138 (MQ=255)
                                                                      ttCGCGACGCTCAACCAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAg                                                                 <  2:1006448/138‑1 (MQ=255)
                                                                         gcgACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAg                                                            <  1:813378/140‑1 (MQ=255)
                                                                           gACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTg                                                          >  2:239561/1‑140 (MQ=255)
                                                                            aCGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTgg                                                         <  1:247454/140‑1 (MQ=255)
                                                                               cTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCAt                                                      <  2:874712/140‑1 (MQ=255)
                                                                                tCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATc                                                     >  2:677914/1‑140 (MQ=255)
                                                                                tCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATc                                                     >  2:932296/1‑140 (MQ=255)
                                                                                    cGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGAc                                                 <  1:1019924/140‑1 (MQ=255)
                                                                                       aaaaaaCCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCAc                                              <  2:534385/140‑1 (MQ=255)
                                                                                       aaaaaaCCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCAc                                              <  2:551880/140‑1 (MQ=255)
                                                                                               tAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCtt                                      >  1:1072077/1‑140 (MQ=255)
                                                                                                      ccGGTACATGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCtttt                               >  2:60929/1‑140 (MQ=255)
                                                                                                             aTGCTGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACAt                        <  1:60929/140‑1 (MQ=255)
                                                                                                                 tGTACTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGc                    >  1:948179/1‑140 (MQ=255)
                                                                                                                    aCTGACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAg                 >  2:603210/1‑140 (MQ=255)
                                                                                                                       gACGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAGACa              <  1:932296/140‑1 (MQ=255)
                                                                                                                        aCGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAGACAt             >  2:1054579/1‑140 (MQ=255)
                                                                                                                        aCGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAGACAt             >  1:177460/1‑140 (MQ=255)
                                                                                                                        aCGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAGACAt             >  1:22505/1‑140 (MQ=255)
                                                                                                                        aCGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAGACAt             >  1:301727/1‑140 (MQ=255)
                                                                                                                        aCGGCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAGACAt             >  2:1066650/1‑140 (MQ=255)
                                                                                                                          ggCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAGACATTg           >  2:829294/1‑140 (MQ=255)
                                                                                                                           gCGACAAATCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAGACATTGc          >  1:1027369/1‑140 (MQ=255)
                                                                                                                             gataaaTCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAGACATTGccc        >  1:659737/4‑140 (MQ=255)
                                                                                                                                   tCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAATTTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAGACATTGCCCAGGCAc  <  2:1027369/140‑1 (MQ=255)
                                                                                                                                   tCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAGACATTGCCCAGGCAc  <  1:677914/140‑1 (MQ=255)
                                                                                                                                   tCCACTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAGACATTGCCCAGGCAc  <  2:642864/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                        
CAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAGCACGTCCACACAGTGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAGACATTGCCCAGGCAC  >  NC_000913/1951848‑1952118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A