Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,124,657	C→T	V364I (GTC→ATC)	wcaI ←	putative colanic biosynthesis glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,124,657	0	C	T	100.0%	178.1 / NA	51	V364I (GTC→ATC)	wcaI	putative colanic biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/25); total (26/25)

ATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGC  >  NC_000913/2124525‑2124784

aTCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTcc                                                                                                                          >  2:544639/1‑140 (MQ=255)

       aaTATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCaa                                                                                                                   >  1:725579/1‑140 (MQ=255)

        atatCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAAc                                                                                                                  >  1:893753/1‑140 (MQ=255)

                 ataAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGc                                                                                                         >  1:139475/1‑140 (MQ=255)

                 ataAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGc                                                                                                         <  1:634844/140‑1 (MQ=255)

                                          tATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGc                                                                                >  1:1090816/1‑140 (MQ=255)

                                          tATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGc                                                                                <  1:152856/140‑1 (MQ=255)

                                          tATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGc                                                                                <  2:69746/140‑1 (MQ=255)

                                            tCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGc                                                                                >  2:90230/1‑138 (MQ=255)

                                            tCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGc                                                                                <  1:90230/138‑1 (MQ=255)

                                              gAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGcc                                                                            <  1:1096600/140‑1 (MQ=255)

                                               aGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGccc                                                                           <  1:128190/140‑1 (MQ=255)

                                               aGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGccc                                                                           <  2:1090816/140‑1 (MQ=255)

                                                gcgtgcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGc                                                                                >  1:1058851/1‑134 (MQ=255)

                                                gcgtgcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGc                                                                                <  2:1058851/134‑1 (MQ=255)

                                                gcgtgcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGcccc                                                                          >  1:818733/1‑140 (MQ=255)

                                                gcgtgcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGcccc                                                                          >  2:304723/1‑140 (MQ=255)

                                                gcgtgcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGcccc                                                                          >  1:954904/1‑140 (MQ=255)

                                                 cgtgcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCa                                                                         >  2:1058459/1‑140 (MQ=255)

                                                  gtgcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAg                                                                        >  2:124827/1‑140 (MQ=255)

                                                  gtgcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAg                                                                        >  2:263929/1‑140 (MQ=255)

                                                    gcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGtt                                                                      <  1:444784/140‑1 (MQ=255)

                                                    gcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGtt                                                                      <  1:356800/140‑1 (MQ=255)

                                                    gcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGtt                                                                      <  2:725579/140‑1 (MQ=255)

                                                         cGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGc                                                                                <  2:253850/125‑1 (MQ=255)

                                                         cGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGc                                                                                >  1:253850/1‑125 (MQ=255)

                                                         cGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCtgtg                                                                 <  2:714984/140‑1 (MQ=255)

                                                         cGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCtgtg                                                                 <  2:954904/140‑1 (MQ=255)

                                                                      tGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTcagcag                                                    >  1:665979/1‑140 (MQ=255)

                                                                          aCCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAGCCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCtt                                                          <  2:315822/130‑1 (MQ=255)

                                                                          aCCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCtt                                                          >  1:315822/1‑130 (MQ=255)

                                                                                   tgttTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGtt                                       >  1:155781/1‑140 (MQ=255)

                                                                                   tgttTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGtt                                       >  1:724046/1‑140 (MQ=255)

                                                                                     tttGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTAc                                     <  2:818733/140‑1 (MQ=255)

                                                                                     tttGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTAc                                     >  1:533312/1‑140 (MQ=255)

                                                                                        gggCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGc                                  >  1:763977/1‑140 (MQ=255)

                                                                                           cagcagGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTa                               <  1:283247/140‑1 (MQ=255)

                                                                                             gcagGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTa                               >  2:1004421/1‑138 (MQ=255)

                                                                                             gcagGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTa                               <  1:1004421/138‑1 (MQ=255)

                                                                                               agGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGc                           <  1:544639/140‑1 (MQ=255)

                                                                                                        tGACGGATCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATAtt                  >  2:99990/1‑140 (MQ=255)

                                                                                                               tCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGaa                      <  2:183767/129‑1 (MQ=255)

                                                                                                               tCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGaa                      >  1:183767/1‑129 (MQ=255)

                                                                                                               tCCCCGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGt           <  2:724046/140‑1 (MQ=255)

                                                                                                                 cccGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGttt         >  2:1043928/1‑140 (MQ=255)

                                                                                                                 cccGCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGttt         >  1:444783/1‑140 (MQ=255)

                                                                                                                    gCCACCAGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGa      <  1:263929/140‑1 (MQ=255)

                                                                                                                     ccaccaGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGAc     <  2:665979/140‑1 (MQ=255)

                                                                                                                     ccaccaGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGAc     <  1:99990/140‑1 (MQ=255)

                                                                                                                       accaGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACgg   <  2:763977/140‑1 (MQ=255)

                                                                                                                        ccaGCGCCTCGATCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGc  >  2:227029/1‑140 (MQ=255)

ATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGC  >  NC_000913/2124525‑2124784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A