Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,430,190	T→C	I17V (ATT→GTT)	purF ←	amidophosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,430,190	0	T	C	100.0%	190.7 / NA	54	I17V (ATT→GTT)	purF	amidophosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (30/24); total (30/24)

CTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAAC  >  NC_000913/2430053‑2430324

cTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCg                                                                                                                                      >  2:1115294/1‑140 (MQ=255)

         gCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTaa                                                                                                                             >  1:1030772/1‑140 (MQ=255)

         gCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTaa                                                                                                                             >  2:1098786/1‑140 (MQ=255)

          cGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAAc                                                                                                                            <  1:1115294/140‑1 (MQ=255)

                  aaaTACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATaa                                                                                                                    >  2:961703/1‑140 (MQ=255)

                        aTCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAAc                                                                                                                            >  1:847918/1‑126 (MQ=255)

                        aTCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAAc                                                                                                                            <  2:847918/126‑1 (MQ=255)

                        aTCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCgg                                                                                                              >  1:104551/1‑140 (MQ=255)

                         tCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGc                                                                                                             >  1:214212/1‑140 (MQ=255)

                            cTCACCAGCCCGTTCGCTTTTCGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGAt                                                                                                          <  1:1104051/140‑1 (MQ=255)

                            cTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGAt                                                                                                          >  2:917710/1‑140 (MQ=255)

                            cTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGAt                                                                                                          >  2:754383/1‑140 (MQ=255)

                                 cAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGa                                                                                                     <  1:122071/140‑1 (MQ=255)

                                         tCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCg                                                                                             <  2:477960/140‑1 (MQ=255)

                                         tCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCg                                                                                             <  2:214212/140‑1 (MQ=255)

                                               tACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATa                                                                                       <  1:961703/140‑1 (MQ=255)

                                                               gTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGtt                                                                       <  1:797772/140‑1 (MQ=255)

                                                                ttATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCt                                                                           <  1:27096/135‑1 (MQ=255)

                                                                ttATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCt                                                                           >  2:27096/1‑135 (MQ=255)

                                                                ttATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTa                                                                      <  1:1098786/140‑1 (MQ=255)

                                                                ttATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTa                                                                      <  1:917710/140‑1 (MQ=255)

                                                                     ggCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCca                                                                 <  2:1030772/140‑1 (MQ=255)

                                                                          cTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACAtct                                                            >  2:1052194/1‑140 (MQ=255)

                                                                          cTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACAtct                                                            >  1:496877/1‑140 (MQ=255)

                                                                            aTGGTGATGATGCCGGCGGCATCCTGACCGAGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGcc                                                                  >  1:594764/1‑132 (MQ=255)

                                                                            aTGGTGATGATGCCGGCGGCATCCTGACCGAGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGcc                                                                  <  2:594764/132‑1 (MQ=255)

                                                                               gTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCagag                                                       <  2:496877/140‑1 (MQ=255)

                                                                               gTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCagag                                                       <  2:637676/140‑1 (MQ=255)

                                                                                tgatgaTGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCagag                                                       <  2:455502/139‑1 (MQ=255)

                                                                                tgatgaTGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCagag                                                       >  1:455502/1‑139 (MQ=255)

                                                                                   tgatgCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACt                                                   >  2:1101504/1‑140 (MQ=255)

                                                                                    gatgCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACtt                                                  >  1:103677/1‑140 (MQ=255)

                                                                                     atgCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTa                                                 >  2:701669/1‑140 (MQ=255)

                                                                                     atgCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTa                                                 >  1:26600/1‑140 (MQ=255)

                                                                                     atgCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTa                                                 >  2:166000/1‑140 (MQ=255)

                                                                                     atgCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTa                                                 >  2:60851/1‑140 (MQ=255)

                                                                                     atgCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTa                                                 >  1:43763/1‑140 (MQ=255)

                                                                                     atgCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTa                                                 >  1:215356/1‑140 (MQ=255)

                                                                                     atgCCGGCGGCATCCTGACCGCGATGCTGAAGAACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTa                                                 >  1:712345/1‑140 (MQ=255)

                                                                                         cggcggCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGct                                             <  2:104551/140‑1 (MQ=255)

                                                                                         cggcggCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGct                                             <  1:754383/140‑1 (MQ=255)

                                                                                         cggcggCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGct                                             <  1:166000/140‑1 (MQ=255)

                                                                                                    tGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGaa                                  <  1:899936/140‑1 (MQ=255)

                                                                                                    tGACCGCGATGCTGAAGAACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGaa                                  <  2:712345/140‑1 (MQ=255)

                                                                                                           gATGCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGAc                           <  2:755212/140‑1 (MQ=255)

                                                                                                              gCTGAAGCACCGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAg                        <  2:215356/140‑1 (MQ=255)

                                                                                                                       ccGTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGa               >  1:27827/1‑140 (MQ=255)

                                                                                                                         gTTAAGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATa             >  2:20469/1‑140 (MQ=255)

                                                                                                                            aaGGCATCATAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATc          <  1:1101787/140‑1 (MQ=255)

                                                                                                                                  tcatAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCaaaaaa    >  1:737447/1‑140 (MQ=255)

                                                                                                                                   catAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTCTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCaaaaaaa   >  1:252080/1‑140 (MQ=255)

                                                                                                                                    atAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAAc  >  2:121563/1‑140 (MQ=255)

                                                                                                                                    atAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAAc  >  2:960255/1‑140 (MQ=255)

                                                                                                                                    atAAACCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAAc  >  1:993658/1‑140 (MQ=255)

CTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAAC  >  NC_000913/2430053‑2430324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A