Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F28 I1 R1
|
180 |
66.5 |
2263572 |
99.4% |
2249990 |
138.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,767,507 |
(C)7→8 |
intergenic (+152/‑203) |
rnlB → / → yfjP |
CP4‑57 prophage; antitoxin RnlB/CP4‑57 prophage; putative GTP‑binding protein YfjP |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,767,500 | 1 | . | C | 97.2%
| 118.9
/ ‑3.9
| 37 | intergenic (+145/‑210) | rnlB/yfjP | CP4‑57 prophage; antitoxin RnlB/CP4‑57 prophage; putative GTP‑binding protein YfjP |
| Reads supporting (aligned to +/- strand): ref base . (1/0); major base C (16/19); minor base A (1/0); total (18/19) |
| Fisher's exact test for biased strand distribution p-value = 4.72e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.40e-01 |
TTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCA > NC_000913/2767365‑2767634
|
ttctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑ccc‑c < 2:714338/140‑1 (MQ=255)
aCCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTaaa < 1:938337/140‑1 (MQ=255)
aCCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCACCTCAAAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCACCCCATTaaa > 2:833148/1‑140 (MQ=255)
taGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCACCCTCACAACGTCCTGCATAGCGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCa > 2:200/1‑140 (MQ=255)
aaTTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCaata > 2:691854/1‑140 (MQ=255)
gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTaaa < 1:257504/125‑1 (MQ=255)
gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTaaa > 2:257504/1‑125 (MQ=255)
ttAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAAt < 1:1083484/140‑1 (MQ=255)
tGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCGGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTAACCAATTTTCTGGAAATCCTatg > 2:788319/1‑140 (MQ=255)
tCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTTTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgat > 2:947600/1‑140 (MQ=255)
tCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTGAACTTCTCATTAATGTCACCAATTTTCTGGAAATCCTatgat > 2:757898/1‑140 (MQ=255)
tCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgat > 1:1034231/1‑140 (MQ=255)
tatGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCtt < 1:3633/140‑1 (MQ=255)
tCAAATAGCATTAACCCCCCCTCCCAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCCCCCCCGGCTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAAATTTCTGGAAATCCTATGATGCCTTTCCCg < 1:570435/140‑1 (MQ=255)
aTTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGt > 2:725356/1‑140 (MQ=255)
aCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTCTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCtg > 1:791851/1‑140 (MQ=255)
cccTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGt > 2:535293/1‑140 (MQ=255)
tGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTg > 2:30260/1‑140 (MQ=255)
gCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGc < 2:947932/140‑1 (MQ=255)
gCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGc > 1:639076/1‑140 (MQ=255)
gCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGc < 2:303871/140‑1 (MQ=255)
gCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGc < 2:60058/140‑1 (MQ=255)
ggAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGa < 1:456556/128‑1 (MQ=255)
ggAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGa > 2:456556/1‑128 (MQ=255)
ggAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTa > 2:48400/1‑140 (MQ=255)
gAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTAt < 2:1034231/140‑1 (MQ=255)
ttCCCCTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGa > 1:337420/1‑140 (MQ=255)
ccccTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGaaa < 1:725356/140‑1 (MQ=255)
cccTGTGCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGaaaa < 1:540279/140‑1 (MQ=255)
gtgCACCCACGACTAAATTTACCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATc > 2:736827/1‑140 (MQ=255)
gCACCCACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCACTGAAAAAATCtt < 2:97621/140‑1 (MQ=255)
ccACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCaga < 2:709201/140‑1 (MQ=255)
cACGACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCagat < 2:791851/140‑1 (MQ=255)
gACTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCagataga < 1:833148/140‑1 (MQ=255)
aCTAAATTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCagatagat > 1:228324/1‑140 (MQ=255)
aaTTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCGGATAGATATCa < 1:200/140‑1 (MQ=255)
aaTTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCa < 2:228324/140‑1 (MQ=255)
aaTTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCa < 1:535293/140‑1 (MQ=255)
aaTTTCCCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCa < 1:48400/140‑1 (MQ=255)
|
TTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCC‑CCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCA > NC_000913/2767365‑2767634
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
