Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,942,298	A→G	R90R (CGT→CGC)	gcvA ←	DNA‑binding transcriptional dual regulator GcvA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,942,298	0	A	G	100.0%	148.7 / NA	43	R90R (CGT→CGC)	gcvA	DNA‑binding transcriptional dual regulator GcvA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/24); total (19/24)

CTTCCTGACGATCAACCGCCTGGATTCGAACGTCAATTCCCGGATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGAGTGAACGATTACGGCGGCGGAACAGTTTTAGCCCCAAAAAATCCT  >  NC_000913/2942165‑2942434

cTTCCTGGCGATCAACCGCCTGGATTCGAACGTCAATTCCCGGATAAGCTGAATTAAAGCTGGAGAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGcc                                                                                                                                    <  2:635104/140‑1 (MQ=255)

     tGACGATCAACCGCCTGGATTCGAACGTCAATTCCCGGATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCt                                                                                                                                   >  2:345035/1‑136 (MQ=255)

     tGACGATCAACCGCCTGGATTCGAACGTCAATTCCCGGATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCt                                                                                                                                   <  1:345035/136‑1 (MQ=255)

            cAACCGCCTGGATTCGAACGTCAATTCCCGGATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTAcgc                                                                                                                        >  2:349420/1‑140 (MQ=255)

             aaCCGCCTGGATTCGAACGTCAATTCCCGGATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTAcgcg                                                                                                                       <  1:382050/140‑1 (MQ=255)

             aaCCGCCTGGATTCGAACGTCAATTCCCGGATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTAcgcg                                                                                                                       <  2:748026/140‑1 (MQ=255)

              aCCGCCTGGATTCGAACGTCAATTCCCGGATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGt                                                                                                                      <  2:811940/140‑1 (MQ=255)

                  ccTGGATTCGAACGTCAATTCCCGGATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCt                                                                                                                  <  2:167765/140‑1 (MQ=255)

                   cTGGATTCGAACGTCAATTCCCGGATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCg                                                                                                                    >  2:728404/1‑137 (MQ=255)

                   cTGGATTCGAACGTCAATTCCCGGATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCg                                                                                                                    <  1:728404/137‑1 (MQ=255)

                    tGGATTCGAACGTCAATTCCCGGATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTc                                                                                                                >  1:322206/1‑140 (MQ=255)

                     ggATTCGAACGTCAATTCCCGGATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCg                                                                                                               >  1:1033871/1‑140 (MQ=255)

                         tCGAACGTCAATTCCCGGATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTa                                                                                                           <  2:827421/140‑1 (MQ=255)

                               gTCAATTCCCGGATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCg                                                                                                     <  2:255554/140‑1 (MQ=255)

                                tCAATTCCCGGATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGa                                                                                                    >  2:937035/1‑140 (MQ=255)

                                  aaTTCCCGGATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGaaa                                                                                                  >  1:204711/1‑140 (MQ=255)

                                      cccGGATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAatat                                                                                              <  1:615691/140‑1 (MQ=255)

                                      cccGGATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAatat                                                                                              <  1:356940/140‑1 (MQ=255)

                                      cccGGATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAatat                                                                                              <  1:1016445/140‑1 (MQ=255)

                                          gATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATctct                                                                                          <  2:683230/140‑1 (MQ=255)

                                                    aTTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAatat                                                                                              >  1:707352/1‑126 (MQ=255)

                                                    aTTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAatat                                                                                              <  2:707352/126‑1 (MQ=255)

                                                    aTTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGa                                                                                <  1:712749/140‑1 (MQ=255)

                                                         aGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGaaa                                                                           >  2:21218/1‑140 (MQ=255)

                                                         aGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGaaa                                                                           >  1:1024608/1‑140 (MQ=255)

                                                             ggAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGc                                                                       >  2:914094/1‑140 (MQ=255)

                                                             ggAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGc                                                                       >  1:928617/1‑140 (MQ=255)

                                                             ggAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCATCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGc                                                                       >  1:192983/1‑140 (MQ=255)

                                                              gAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCt                                                                      <  1:21218/140‑1 (MQ=255)

                                                               aaaGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCtt                                                                     <  1:937035/140‑1 (MQ=255)

                                                                           ccaaccaaTGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCg                                                         <  1:349420/140‑1 (MQ=255)

                                                                           ccaaccaaTGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCg                                                         <  1:187403/140‑1 (MQ=255)

                                                                              accaaTGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTc                                                      <  2:98033/140‑1 (MQ=255)

                                                                                   tgaatgGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTcagcag                                                 >  1:475575/1‑140 (MQ=255)

                                                                                        ggCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGAGTg                                            <  2:1033871/140‑1 (MQ=255)

                                                                                        ggCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGAGTg                                            >  1:670032/1‑140 (MQ=255)

                                                                                        ggCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGAGTg                                            >  1:1066606/1‑140 (MQ=255)

                                                                                                              aCCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGAGTGAACGATTACGGCGGCGGAACAg                      >  1:904130/1‑140 (MQ=255)

                                                                                                              aCCGTCAACGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGAGTGAACGATTACGGCGGCGGAACAg                      >  1:534614/1‑140 (MQ=255)

                                                                                                                      cGCCCCCTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGAGTGAACGATTACGGCGGCGGAACAGTTTTAGcc              <  2:204711/140‑1 (MQ=255)

                                                                                                                            cTTGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGAGTGAACGATTACGGCGGCGGAACAGTTTTAGCCCCaaaa        >  2:576669/1‑140 (MQ=255)

                                                                                                                             ttGGCGCTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGAGTGAACGATTACGGCGGCGGAACAGTTTTAGCCCCaaaaa       <  2:904130/140‑1 (MQ=255)

                                                                                                                                  gcTGCGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGAGTGAACGATTACGGCGGCGGAACAGTTTTAGCCCCAAAAAATCCt  <  1:576669/140‑1 (MQ=255)

CTTCCTGACGATCAACCGCCTGGATTCGAACGTCAATTCCCGGATAAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGAGTGAACGATTACGGCGGCGGAACAGTTTTAGCCCCAAAAAATCCT  >  NC_000913/2942165‑2942434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A