Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,108,796	G→A	C120C (TGC→TGT)	speC ←	ornithine decarboxylase, biosynthetic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,108,796	0	G	A	100.0%	111.7 / NA	33	C120C (TGC→TGT)	speC	ornithine decarboxylase, biosynthetic
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/18); total (15/18)

GAATAAGCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTGGCAGCAAATTCTCTTCATACTGACAGGCTGCGGATTCCAGCTCCAGCCACTGCTGCTCGTTGCCGTTGATTACCGCC  >  NC_000913/3108666‑3108931

gAATAAGCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAg                                                                                                                                <  1:693286/140‑1 (MQ=255)

   tAAGCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTg                                                                                                                             >  2:903801/1‑140 (MQ=255)

     aGCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCt                                                                                                                           >  1:482109/1‑140 (MQ=255)

          aTCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGc                                                                                                                      >  1:326470/1‑140 (MQ=255)

          aTCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGAATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGc                                                                                                                      >  1:243993/1‑140 (MQ=255)

           tCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGcc                                                                                                                     <  2:678863/140‑1 (MQ=255)

            cGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGccc                                                                                                                    >  2:317211/1‑140 (MQ=255)

             gCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCa                                                                                                                   <  2:722444/140‑1 (MQ=255)

                       aCGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTa                                                                                                         <  2:891440/140‑1 (MQ=255)

                       aCGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTa                                                                                                         <  2:625053/140‑1 (MQ=255)

                       aCGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGAATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTa                                                                                                         <  2:243993/140‑1 (MQ=255)

                                        atCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCAt                                                                                        >  1:268142/1‑140 (MQ=255)

                                        atCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCAt                                                                                        <  1:903801/140‑1 (MQ=255)

                                        atCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTT‑AAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATa                                                                                       >  1:615451/1‑140 (MQ=255)

                                             cgcgAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCgt                                                                                             <  1:427706/130‑1 (MQ=255)

                                             cgcgAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCgt                                                                                             >  2:427706/1‑130 (MQ=255)

                                             cgcgAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAAc                                                                                   >  1:70595/1‑140 (MQ=255)

                                               cgAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACgg                                                                                 >  2:770849/1‑140 (MQ=255)

                                                 aaaGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTg                                                                               <  1:317211/140‑1 (MQ=255)

                                                 aaaGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTg                                                                               <  1:174031/140‑1 (MQ=255)

                                                   aGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTGgc                                                                             <  1:221963/140‑1 (MQ=255)

                                                               cAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTGGCAGCAAATTctct                                                                 <  1:678508/140‑1 (MQ=255)

                                                                  aGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTGGCAGCAAATTCTCTTCa                                                              <  2:268142/140‑1 (MQ=255)

                                                                         gTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTGGCAGCAAATTCTCTTCATACTGAc                                                       <  2:482109/140‑1 (MQ=255)

                                                                         gTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTGGCAGCAAATTCTCTTCATACTGAc                                                       <  2:70595/140‑1 (MQ=255)

                                                                          tAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTGGCAGCAAATTCTCTTCATACTGACa                                                      >  1:334963/1‑140 (MQ=255)

                                                                                           aGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTGGCAGCAAATTCTCTTCATACTGACAGGCTGCGGATtccagct                                     <  1:770849/140‑1 (MQ=255)

                                                                                                  tttttAAAAAACGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTGGCAGCAAATTCTCTTCATACTGACAGGCTGCGGATTCCAGCTCCAGCCa                              >  1:449892/1‑140 (MQ=255)

                                                                                                          aaaCGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTGGCAGCAAATTCTCTTCATACTGACAGGCTGCGGATTCCAGCTCCAGCCACTgctgct                      <  2:449892/140‑1 (MQ=255)

                                                                                                           aaCGCACCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTGGCAGCAAATTCTCTTCATACTGACAGGCTGCGGATTCCAGCTCCAGCCACTGCTGCTc                     >  1:970244/1‑140 (MQ=255)

                                                                                                                aCCATGTTGATGTCCAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTGGCAGCAAATTCTCTTCATACTGACAGGCTGCGGATTCCAGCTCCAGCCACTGCTGCTCGTTGc                <  2:326470/140‑1 (MQ=255)

                                                                                                                              cAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTGGCAGCAAATTCTCTTCATACTGACAGGCTGCGGATTCCAGCTCCAGCCACTGCTGCTCGTTGCCGTTGATTAccgcc  >  1:29541/1‑140 (MQ=255)

                                                                                                                              cAGGACAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTGGCAGCAAATTCTCTTCATACTGACAGGCTGCGGATTCCAGCTCCAGCCACTGCTGCTCGTTGCCGTTGATTAccgcc  >  1:253264/1‑140 (MQ=255)

GAATAAGCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTGGCAGCAAATTCTCTTCATACTGACAGGCTGCGGATTCCAGCTCCAGCCACTGCTGCTCGTTGCCGTTGATTACCGCC  >  NC_000913/3108666‑3108931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A