Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,859,835	T→C	L94L (TTA→TTG)	yidK ←	putative transporter YidK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,859,835	0	T	C	100.0%	113.0 / NA	33	L94L (TTA→TTG)	yidK	putative transporter YidK
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/20); total (13/20)

AGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAG  >  NC_000913/3859704‑3859971

agaACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGAt                                                                                                                                  >  2:402724/1‑140 (MQ=255)

agaACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGAt                                                                                                                                  <  1:402724/140‑1 (MQ=255)

       tCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGcgcg                                                                                                                           >  2:798214/1‑140 (MQ=255)

       tCGGCAGAAACCAGTCGCCGGTGGCAATTAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGcgcg                                                                                                                           <  2:696918/140‑1 (MQ=255)

         ggCAGAAAGCAGACGCCGGTGGCAATTAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAg                                                                                                                         <  2:597716/140‑1 (MQ=255)

                       gCCGGTGGCAATTAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCa                                                                                                           <  1:996928/140‑1 (MQ=255)

                                 aTTAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGt                                                                                                 <  1:407009/140‑1 (MQ=255)

                                 aTTAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGt                                                                                                 >  1:888109/1‑140 (MQ=255)

                                 aTTAGGAAGCAGAAGTCGATGATAATACGCGTCGGTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGTGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGt                                                                                                 <  2:518467/140‑1 (MQ=255)

                                   tAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCa                                                                                               <  1:889293/140‑1 (MQ=255)

                                          cAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCa                                                                                        >  1:249474/1‑140 (MQ=255)

                                           aGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAg                                                                                       <  1:295957/140‑1 (MQ=255)

                                                tCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCAt                                                                                  <  2:597775/140‑1 (MQ=255)

                                                          taCGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATg                                                                        >  2:689673/1‑140 (MQ=255)

                                                          taCGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATg                                                                        >  1:886956/1‑140 (MQ=255)

                                                           aCGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGc                                                                       <  2:249474/140‑1 (MQ=255)

                                                              cgtcgtTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGc                                                                    <  2:1060154/140‑1 (MQ=255)

                                                                tcgtTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCtt                                                                  >  2:1029549/1‑140 (MQ=255)

                                                                 cgtTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGc                                                                    <  1:730429/137‑1 (MQ=255)

                                                                 cgtTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGc                                                                    >  2:730429/1‑137 (MQ=255)

                                                                   ttttATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTg                                                               >  2:340542/1‑140 (MQ=255)

                                                                     ttATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGc                                                                    >  2:680986/1‑133 (MQ=255)

                                                                     ttATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGc                                                                    <  1:680986/133‑1 (MQ=255)

                                                                        tCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGc                                                          <  1:194748/140‑1 (MQ=255)

                                                                                  cctccAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGaa                                                <  1:798214/140‑1 (MQ=255)

                                                                                  cctccAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGaa                                                >  2:699811/1‑140 (MQ=255)

                                                                                  cctccAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGaa                                                <  1:293686/140‑1 (MQ=255)

                                                                                   ctccAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGaaa                                               <  2:460436/140‑1 (MQ=255)

                                                                                                                 gcgcTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttgg                 <  2:888109/140‑1 (MQ=255)

                                                                                                                  cgcTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttggt                <  2:127765/140‑1 (MQ=255)

                                                                                                                  cgcTTCAGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttggt                >  1:127765/1‑140 (MQ=255)

                                                                                                                        aGATAGCGCGGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAt          >  1:808976/1‑140 (MQ=255)

                                                                                                                                cgGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAg  <  1:699811/140‑1 (MQ=255)

                                                                                                                                cgGCAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAg  <  2:808976/140‑1 (MQ=255)

AGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAG  >  NC_000913/3859704‑3859971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A