Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,541,042	A→G	Y517H (TAT→CAT)	narZ ←	nitrate reductase Z subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,541,042	0	A	G	100.0%	181.6 / NA	51	Y517H (TAT→CAT)	narZ	nitrate reductase Z subunit alpha
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (28/23); total (28/23)

CAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCT  >  NC_000913/1540908‑1541177

cAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGgc                                                                                                                                    >  1:415873/1‑140 (MQ=255)

      atTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCa                                                                                                                              <  2:828219/140‑1 (MQ=255)

             atcCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCgg                                                                                                                                >  1:723467/1‑131 (MQ=255)

             atcCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCgg                                                                                                                                <  2:723467/131‑1 (MQ=255)

             atcCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGt                                                                                                                       >  2:859858/1‑140 (MQ=255)

               cccGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGGACGCCGGTaa                                                                                                                     >  1:1170861/1‑140 (MQ=255)

               cccGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTaa                                                                                                                     >  2:21360/1‑140 (MQ=255)

                     gTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCt                                                                                                               <  1:859858/140‑1 (MQ=255)

                     gTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCt                                                                                                               <  1:1192647/140‑1 (MQ=255)

                        gTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCAc                                                                                                            >  2:404579/1‑140 (MQ=255)

                         ttCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCAcc                                                                                                           <  2:97951/140‑1 (MQ=255)

                         ttCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCAcc                                                                                                           >  2:1068027/1‑140 (MQ=255)

                          tCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCAccc                                                                                                          <  1:1068027/140‑1 (MQ=255)

                                ccATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGc                                                                                                                >  2:1017221/1‑128 (MQ=255)

                                ccATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGc                                                                                                                <  1:1017221/128‑1 (MQ=255)

                                ccATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCt                                                                                                               <  2:1000799/129‑1 (MQ=255)

                                ccATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCt                                                                                                               >  1:1000799/1‑129 (MQ=255)

                                ccATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCt                                                                                                    <  1:1080344/140‑1 (MQ=255)

                                ccATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCt                                                                                                    >  2:721762/1‑140 (MQ=255)

                                   tgtgATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTggg                                                                                                 >  1:387734/1‑140 (MQ=255)

                                         aCCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTAc                                                                                           >  1:429039/1‑140 (MQ=255)

                                                gTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGa                                                                                    >  1:426343/1‑140 (MQ=255)

                                                 ttAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGAt                                                                                   >  1:913607/1‑140 (MQ=255)

                                                   aaCACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGAttt                                                                                 <  2:996792/140‑1 (MQ=255)

                                                        cTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCg                                                                            >  1:1058908/1‑140 (MQ=255)

                                                        cTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCg                                                                            >  2:1058180/1‑140 (MQ=255)

                                                            gcCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGCTTGATTTCAGCGTAAt                                                                        <  2:949448/140‑1 (MQ=255)

                                                                aGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCttt                                                                    >  2:475998/1‑140 (MQ=255)

                                                                  gATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCg                                                                  <  2:1058908/140‑1 (MQ=255)

                                                                    tAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCa                                                                <  1:1058180/140‑1 (MQ=255)

                                                                    tAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCa                                                                <  1:21360/140‑1 (MQ=255)

                                                                    tAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCa                                                                <  2:387734/140‑1 (MQ=255)

                                                                     aaTCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCAc                                                               >  1:569510/1‑140 (MQ=255)

                                                                       tcatcGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTg                                                             <  2:301052/140‑1 (MQ=255)

                                                                            gAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTc                                                        >  2:1199187/1‑140 (MQ=255)

                                                                            gAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTc                                                        >  1:1131445/1‑140 (MQ=255)

                                                                                 cccATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCtt                                                   >  1:991474/1‑140 (MQ=255)

                                                                                 cccATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCtt                                                   >  1:375395/1‑140 (MQ=255)

                                                                                       cGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTAAGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTc                                                  <  1:766204/135‑1 (MQ=255)

                                                                                       cGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTAAGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTc                                                  >  2:766204/1‑135 (MQ=255)

                                                                                            tATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGa                                        >  1:260135/1‑140 (MQ=255)

                                                                                              tGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATc                                      >  1:952103/1‑140 (MQ=255)

                                                                                              tGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATc                                      <  1:512965/140‑1 (MQ=255)

                                                                                               gggCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCg                                     <  2:429039/140‑1 (MQ=255)

                                                                                                ggCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGa                                    >  2:243385/1‑140 (MQ=255)

                                                                                                          gCAAATTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAAtt                          >  1:187432/1‑140 (MQ=255)

                                                                                                             aaTTCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGc                       <  2:1131445/140‑1 (MQ=255)

                                                                                                               ttCACGGGCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCa                     >  2:695572/1‑140 (MQ=255)

                                                                                                                      gCGATGGTTTCAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCaa              <  1:430764/140‑1 (MQ=255)

                                                                                                                                cAATATGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACg    <  1:693867/140‑1 (MQ=255)

                                                                                                                                  atatGCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCt  <  2:260135/140‑1 (MQ=255)

CAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCT  >  NC_000913/1540908‑1541177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A