Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,824,706	T→C	T78A (ACC→GCC)	ves ←	HutD family protein Ves

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,824,706	0	T	C	100.0%	162.0 / NA	46	T78A (ACC→GCC)	ves	HutD family protein Ves
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/27); total (19/27)

TTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAA  >  NC_000913/1824569‑1824836

ttCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCAt                                                                                                                                  >  1:1143484/1‑140 (MQ=255)

ttCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCAt                                                                                                                                  >  2:247006/1‑140 (MQ=255)

    gCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGtt                                                                                                                              >  2:1062936/1‑140 (MQ=255)

     cAATTCTGACTTTCCCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTa                                                                                                                             >  2:1188278/1‑140 (MQ=255)

        ttCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAg                                                                                                                          <  1:1233228/140‑1 (MQ=255)

               tttCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTg                                                                                                                   <  1:1062936/140‑1 (MQ=255)

                 tCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTgcg                                                                                                                 <  1:29102/140‑1 (MQ=255)

                 tCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTgcg                                                                                                                 <  2:1236773/140‑1 (MQ=255)

                   gCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCt                                                                                                               <  2:342058/140‑1 (MQ=255)

                              ttCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCa                                                                                                    <  1:543470/139‑1 (MQ=255)

                               tCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCAt                                                                                                   >  2:520036/1‑140 (MQ=255)

                                 cAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCAtct                                                                                                 >  2:174244/1‑140 (MQ=255)

                                    gCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTcgc                                                                                              <  2:242118/140‑1 (MQ=255)

                                      gtgtCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTcgccg                                                                                            <  1:520036/140‑1 (MQ=255)

                                      gtgtCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTcgccg                                                                                            >  2:82354/1‑140 (MQ=255)

                                                gTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCa                                                                                  <  1:62101/140‑1 (MQ=255)

                                                gTTGAAATCCATCGACATCTGCCCTGCCGTCAGATTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGC‑GGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCaa                                                                                 <  2:46616/140‑1 (MQ=255)

                                                 ttGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCaa                                                                                 <  2:1143484/140‑1 (MQ=255)

                                                 ttGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCaa                                                                                 <  1:1084836/140‑1 (MQ=255)

                                                 ttGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCaa                                                                                 <  1:446654/140‑1 (MQ=255)

                                                   gAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGACTTCCAGCAACg                                                                               <  2:65702/140‑1 (MQ=255)

                                                      aTCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCa                                                                            >  1:418408/1‑140 (MQ=255)

                                                        ccATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACt                                                                          <  2:444318/140‑1 (MQ=255)

                                                        ccATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACt                                                                          <  2:623450/140‑1 (MQ=255)

                                                         cATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTa                                                                         >  1:888573/1‑140 (MQ=255)

                                                          aTCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTAt                                                                        >  2:595327/1‑140 (MQ=255)

                                                          aTCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTAt                                                                        <  2:671489/140‑1 (MQ=255)

                                                           tCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGc                                                                                   >  2:983711/1‑128 (MQ=255)

                                                           tCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGc                                                                                   <  1:983711/128‑1 (MQ=255)

                                                                               aGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACaa                                                   <  2:418408/140‑1 (MQ=255)

                                                                                    cGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGaaa                                              <  1:595327/140‑1 (MQ=255)

                                                                                    cGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGaaa                                              >  2:673194/1‑140 (MQ=255)

                                                                                               ccTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTc                                   >  1:733527/1‑140 (MQ=255)

                                                                                                   gTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTcgccg                               >  1:170720/1‑140 (MQ=255)

                                                                                                     ccGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCcgcg                             <  1:673194/140‑1 (MQ=255)

                                                                                                       gCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCgat                           >  1:827539/1‑140 (MQ=255)

                                                                                                       gCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCgat                           <  2:733760/140‑1 (MQ=255)

                                                                                                        cTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCgatg                          >  2:843731/1‑140 (MQ=255)

                                                                                                          gCAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCgatgga                        >  1:1204440/1‑140 (MQ=255)

                                                                                                           cAAAGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCgatggat                       <  1:857286/140‑1 (MQ=255)

                                                                                                              aGGCAAAAGGCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCa                    >  2:1172014/1‑140 (MQ=255)

                                                                                                                       gCTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCa           <  2:170720/140‑1 (MQ=255)

                                                                                                                        cTGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCAc          <  1:1172014/140‑1 (MQ=255)

                                                                                                                         tGGAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACg         <  2:827539/140‑1 (MQ=255)

                                                                                                                          ggAACGGTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGc        <  2:733527/140‑1 (MQ=255)

                                                                                                                                gTTTTAAGGCATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTaa  >  1:917814/1‑140 (MQ=255)

TTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAA  >  NC_000913/1824569‑1824836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A