Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,886,730	G→A	D296N (GAT→AAT)	yeaX →	carnitine monooxygenase subunit YeaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,886,730	0	G	A	100.0%	133.9 / NA	38	D296N (GAT→AAT)	yeaX	carnitine monooxygenase subunit YeaX
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (12/26); total (12/26)

ATGACTATTTTGCAGGTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTA  >  NC_000913/1886598‑1886866

aTGACTATTTTGCAGGTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAaga                                                                                                                                   <  1:871739/140‑1 (MQ=255)

        tttGCAGGTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTgcca                                                                                                                           <  1:838667/140‑1 (MQ=255)

        tttGCAGGTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTgcca                                                                                                                           >  2:838667/1‑140 (MQ=255)

          tGCAGGTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTgccagc                                                                                                                         <  1:48651/140‑1 (MQ=255)

            cAGGTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTgccagcca                                                                                                                       <  1:356336/140‑1 (MQ=255)

            cAGGTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTgccagcca                                                                                                                       <  1:5448/140‑1 (MQ=255)

             aGGTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGccagccag                                                                                                                      <  1:988287/140‑1 (MQ=255)

               gTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCa                                                                                                                    <  2:798963/140‑1 (MQ=255)

               gTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCa                                                                                                                    <  2:1125151/140‑1 (MQ=255)

               gTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCa                                                                                                                    <  2:111770/140‑1 (MQ=255)

               gTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCa                                                                                                                    <  1:623084/140‑1 (MQ=255)

                ttATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCaa                                                                                                                   <  2:820121/140‑1 (MQ=255)

                 tATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCaaa                                                                                                                  <  1:700876/140‑1 (MQ=255)

                 tATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCaaa                                                                                                                  >  1:305928/1‑140 (MQ=255)

                    tGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTa                                                                                                               <  1:643959/140‑1 (MQ=255)

                            ataaAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATc                                                                                                       <  1:936684/140‑1 (MQ=255)

                                  ccGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCtgttgt                                                                                                 >  1:724576/1‑140 (MQ=255)

                                            ggAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGa                                                                                       <  1:1216419/140‑1 (MQ=255)

                                                   ttATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTaa                                                                                <  2:305928/140‑1 (MQ=255)

                                                    tATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATA‑TTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGGGTGCGAAGGGTAAAc                                                                              <  1:388355/140‑1 (MQ=255)

                                                       gTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGc                                                                            <  1:422903/140‑1 (MQ=255)

                                                        tCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGcc                                                                           >  1:219100/1‑140 (MQ=255)

                                                            gAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGgt                                                                       >  2:1110278/1‑140 (MQ=255)

                                                                   tATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGAt                                                                <  2:724576/140‑1 (MQ=255)

                                                                               gCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCCATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGcc                                                    >  2:620657/1‑140 (MQ=255)

                                                                               gCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTAcc                                                    <  2:457614/140‑1 (MQ=255)

                                                                                cGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCg                                                   <  2:516508/140‑1 (MQ=255)

                                                                                                gAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGgcgc                                   <  2:219100/140‑1 (MQ=255)

                                                                                                  aGGTGAAGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTg                                 >  2:537086/1‑140 (MQ=255)

                                                                                                       aaGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAg                               <  1:252653/137‑1 (MQ=255)

                                                                                                       aaGCTGACCATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAg                               >  2:252653/1‑137 (MQ=255)

                                                                                                              ccATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGgcgc                                   >  2:261635/1‑126 (MQ=255)

                                                                                                              ccATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGgcgc                                   <  1:261635/126‑1 (MQ=255)

                                                                                                               cATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAgcgcgc                          >  1:488711/1‑134 (MQ=255)

                                                                                                               cATCGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAgcgcgc                          <  2:488711/134‑1 (MQ=255)

                                                                                                                  cGGGATCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCgg                 >  1:214011/1‑140 (MQ=255)

                                                                                                                       tCAATATTTTAGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCg            >  1:899843/1‑140 (MQ=255)

                                                                                                                                 aGCAATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTa  <  1:537086/140‑1 (MQ=255)

ATGACTATTTTGCAGGTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTA  >  NC_000913/1886598‑1886866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A