Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,015,611	T→A	D244E (GAT→GAA)	fliG →	flagellar motor switch protein FliG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,015,611	0	T	A	100.0%	165.7 / NA	48	D244E (GAT→GAA)	fliG	flagellar motor switch protein FliG
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (20/28); total (20/28)

GAAATTATCAACCTGATGAAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTG  >  NC_000913/2015480‑2015737

gAAATTATCAACCTGATGAAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCAt                                                                                                                        <  2:1096322/140‑1 (MQ=255)

gAAATTATCAACCTGATGAAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCAt                                                                                                                        <  1:721146/140‑1 (MQ=255)

   aTTATCAACCTGATGAAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCa                                                                                                                     <  1:767509/140‑1 (MQ=255)

   aTTATCAACCTGATGAAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCa                                                                                                                     <  2:487172/140‑1 (MQ=255)

     tATCAACCTGATGAAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGc                                                                                                                   <  1:814507/140‑1 (MQ=255)

              gatgaAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGt                                                                                                                 >  2:218888/1‑133 (MQ=255)

              gatgaAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGt                                                                                                                 <  1:218888/133‑1 (MQ=255)

              gatgaAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGc                                                                                                          >  1:1020653/1‑140 (MQ=255)

              gatgaAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGc                                                                                                          <  2:423946/140‑1 (MQ=255)

                 gaAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAgg                                                                                                       <  2:1127762/140‑1 (MQ=255)

                    aaCTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCt                                                                                                               >  1:1021347/1‑129 (MQ=255)

                    aaCTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCt                                                                                                               <  2:1021347/129‑1 (MQ=255)

                     aCTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGt                                                                                                   <  2:1020653/140‑1 (MQ=255)

                     aCTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGt                                                                                                   <  2:120151/140‑1 (MQ=255)

                      cTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTg                                                                                                  >  1:1179790/1‑140 (MQ=255)

                         agcagGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGAt                                                                                               >  1:352005/1‑140 (MQ=255)

                         agcagGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGAt                                                                                               >  1:1058616/1‑140 (MQ=255)

                               aagaagCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACACAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGaa                                                                                         <  1:799628/140‑1 (MQ=255)

                                 gaagCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATc                                                                                       <  1:1072689/140‑1 (MQ=255)

                                   agCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGc                                                                                     >  2:1013428/1‑140 (MQ=255)

                                   agCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGc                                                                                     >  2:59502/1‑140 (MQ=255)

                                    gCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCt                                                                                    >  2:1245710/1‑140 (MQ=255)

                                        ttattaCCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTg                                                                                >  2:899919/1‑140 (MQ=255)

                                        ttattaCCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTg                                                                                >  1:1133825/1‑140 (MQ=255)

                                        ttattaCCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTg                                                                                >  2:8090/1‑140 (MQ=255)

                                            taCCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATcg                                                                            >  2:966152/1‑140 (MQ=255)

                                              ccgccgTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATcgcg                                                                          <  1:1013428/140‑1 (MQ=255)

                                              ccgccgTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATcgcg                                                                          <  2:1058616/140‑1 (MQ=255)

                                              ccgccgTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATcgcg                                                                          <  1:59502/140‑1 (MQ=255)

                                                  cgtgcgtgAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGa                                                                      >  1:1190946/1‑140 (MQ=255)

                                                  cgtgcgtgAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGa                                                                      >  2:145709/1‑140 (MQ=255)

                                                      cgtgAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAgg                                                                  >  2:616124/1‑140 (MQ=255)

                                                        tgAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAg                                                                >  2:720175/1‑140 (MQ=255)

                                                        tgAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAg                                                                <  1:696216/140‑1 (MQ=255)

                                                         gAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGc                                                               <  1:1245710/140‑1 (MQ=255)

                                                                aCGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGagcag                                                        >  2:388301/1‑140 (MQ=255)

                                                                          ggCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGcgcg                                              <  1:720175/140‑1 (MQ=255)

                                                                                       atcGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTgcg                                 <  1:145709/140‑1 (MQ=255)

                                                                                       atcGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTgcg                                 <  1:1064244/140‑1 (MQ=255)

                                                                                         cgacgaGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCa                               <  2:352005/140‑1 (MQ=255)

                                                                                         cgacgaGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCa                               <  1:966152/140‑1 (MQ=255)

                                                                                         cgacgaGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCa                               <  1:899919/140‑1 (MQ=255)

                                                                                           acgaGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAat                             <  1:480716/140‑1 (MQ=255)

                                                                                               gaTGTTCCTGTTCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGt                         >  2:749182/1‑140 (MQ=255)

                                                                                                         ttCGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTgc               <  1:8090/140‑1 (MQ=255)

                                                                                                           cGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTgccg             >  2:594077/1‑140 (MQ=255)

                                                                                                           cGAGAATCTGGTGGATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTgccg             <  2:1179790/140‑1 (MQ=255)

                                                                                                                      tggATGTCGACGAACGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTg  <  1:749182/140‑1 (MQ=255)

GAAATTATCAACCTGATGAAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTG  >  NC_000913/2015480‑2015737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A