Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 260 73.5 2514320 99.3% 2496719 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,198,685 A→G intergenic (+1387/‑1594) yehF → / → yehI putative protein YehF/DUF4132 domain‑containing protein YehI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,198,6850AG96.1% 169.1 / ‑4.8 51intergenic (+1387/‑1594)yehF/yehIputative protein YehF/DUF4132 domain‑containing protein YehI
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (22/27);  total (22/29)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAACGCTACGCGAAAACGCCCGTAGCTGGCTGTTGAAATACCCGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTACCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCGATCCCTTAGATAATCACCCGACAAAAATCCCCACTCTTCCGGC  >  NC_000913/2198553‑2198821
                                                                                                                                    |                                                                                                                                        
aaaaCGCTACGCGAAAACGCCCGTAGCTGGCTGTTGAAATACCCGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGaaaa                                                                                                                                   <  2:1004519/140‑1 (MQ=255)
    cGCTACGCGAAAACGCCCGTAGCTGGCTGTTGAAATACCCGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGt                                                                                                                               <  2:693870/140‑1 (MQ=255)
      cTACGCGAAAACGCCCGTAGCTGGCTGTTGAAATACCCGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGtca                                                                                                                             <  2:884671/140‑1 (MQ=255)
      cTACGCGAAAACGCCCGTAGCTGGCTGTTGAAATACCCGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGtca                                                                                                                             <  1:393080/140‑1 (MQ=255)
      cTACGCGAAAACGCCCGTAGCTGGCTGTTGAAATACCCGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTACCGAAAACGGtca                                                                                                                             <  2:1178519/140‑1 (MQ=255)
        aCGCGAAAACGCCCGTAGCTGGCTGTTGAAATACCCGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGtcatc                                                                                                                           <  1:837633/140‑1 (MQ=255)
        aCGCGAAAACGCCCGTAGCTGGCTGTTGAAATACCCGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGtcatc                                                                                                                           >  2:209974/1‑140 (MQ=255)
         cgcgAAAACGCCCGTAGCTGGCTGTTGAAATACCCGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGtcatca                                                                                                                          <  2:892848/140‑1 (MQ=255)
          gcgAAAACGCCCGTAGCTGGCTGTTGAAATACCCGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAg                                                                                                                         >  1:500966/1‑140 (MQ=255)
          gcgAAAACGCCCGTAGCTGGCTGTTGAAATACCCGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAg                                                                                                                         >  2:511873/1‑140 (MQ=255)
                   cccGTAGCTGGCTGTTGAAATACCCGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACg                                                                                                                                 <  2:1129552/123‑1 (MQ=255)
                   cccGTAGCTGGCTGTTGAAATACCCGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACg                                                                                                                                 >  1:1129552/1‑123 (MQ=255)
                    ccGTAGCTGGCTGTTGAAATACCCGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGc                                                                                                               <  2:1251045/140‑1 (MQ=255)
                     cGTAGCTGGCTGTTGAAATACCCGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCa                                                                                                              >  2:700699/1‑140 (MQ=255)
                         gctggctgTTGAAATACCCGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGaa                                                                                                          <  2:500966/140‑1 (MQ=255)
                               tgTTGAAATACCCGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGcc                                                                                                    <  1:514397/140‑1 (MQ=255)
                                gTTGAAATACCCGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGccc                                                                                                   >  2:917872/1‑140 (MQ=255)
                                 ttGAAATACCCGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCg                                                                                                  <  1:938701/140‑1 (MQ=255)
                                 gggAAATACCCGGAACATGCCCTTACCGGCCGGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTACCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCg                                                                                                  <  2:426135/138‑1 (MQ=255)
                                          ccGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATaa                                                                                         <  1:5738/140‑1 (MQ=255)
                                            ggAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAAcc                                                                                       >  2:174050/1‑140 (MQ=255)
                                              aaCATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAg                                                                                     >  2:228148/1‑140 (MQ=255)
                                                   gCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCTGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGa                                                                                >  1:1016545/1‑140 (MQ=255)
                                                   gCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGa                                                                                >  2:465398/1‑140 (MQ=255)
                                                        tACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTaa                                                                           >  2:494778/1‑140 (MQ=255)
                                                              ccTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGc                                                                                    >  2:980190/1‑125 (MQ=255)
                                                              ccTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGc                                                                                    <  1:980190/125‑1 (MQ=255)
                                                                       tgcGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACg                                                            >  2:203238/1‑140 (MQ=255)
                                                                       tgcGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACg                                                            >  1:1017819/1‑140 (MQ=255)
                                                                               tCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCtt                                                    >  2:456237/1‑140 (MQ=255)
                                                                                      aGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCg                                             <  2:1017819/140‑1 (MQ=255)
                                                                                      aGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCg                                             <  1:700699/140‑1 (MQ=255)
                                                                                      aGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCg                                             <  1:228148/140‑1 (MQ=255)
                                                                                      aGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCg                                             <  2:568910/140‑1 (MQ=255)
                                                                                       gctggTGAAGCACAGGATAACGCCCGCGCTCCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCGa                                            <  2:770757/137‑1 (MQ=255)
                                                                                           gTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCGATccc                                        <  1:511873/140‑1 (MQ=255)
                                                                                             gAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCGATCCCtt                                      <  1:494778/140‑1 (MQ=255)
                                                                                                           cGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCTATCCCTTAGATAATCACCCGa                        >  2:566715/1‑140 (MQ=255)
                                                                                                           cGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCGATCCCTTAGATAATCACCCGa                        >  1:22723/1‑140 (MQ=255)
                                                                                                           cGCCCGCGCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCGATCCCTTAGATAATCACCCGa                        >  1:472334/1‑140 (MQ=255)
                                                                                                               cgcgCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCGATCCCTTAGATAATCACCCGACaaa                    <  2:1239476/140‑1 (MQ=255)
                                                                                                               cgcgCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCGATCCCTTAGATAATCACCCGACaaa                    <  2:884477/140‑1 (MQ=255)
                                                                                                               cgcgCTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCGATCCCTTAGATAATCACCCGACaaa                    <  1:771964/140‑1 (MQ=255)
                                                                                                                gcgcTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCGATCCCTTAGATAATCACCCGACaaaa                   >  1:860139/1‑140 (MQ=255)
                                                                                                                gcgcTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCGATCCCTTAGATAATCACCCGACaaaa                   >  2:326672/1‑140 (MQ=255)
                                                                                                                gcgcTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCGATCCCTTAGATAATCACCCGACaaaa                   >  2:265968/1‑140 (MQ=255)
                                                                                                                  gcTGCCTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCGATCCCTTAGATAATCACCCGACAAAAAt                 <  1:209974/140‑1 (MQ=255)
                                                                                                                       cTTGCGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCGATCCCTTAGATAATCACCCGACAAAAATCCCCa            <  1:203238/140‑1 (MQ=255)
                                                                                                                           cGTATGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCGATCCCTTAGATAATCACCCGACAAAAATCCCCActct        >  2:517711/1‑140 (MQ=255)
                                                                                                                             tttGCTTGCCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCGATCCCTTAGATAATCACCCGACAAAAATCCCCACTCTTc      <  2:850705/138‑1 (MQ=255)
                                                                                                                                 cTTGCCGAAAACGGTCATCAGCCATTACTACAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCGATCCCTTAGATAATCACCCGACAAAAATCCCCACTCTTCCGGc  <  2:620012/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                        
AAAACGCTACGCGAAAACGCCCGTAGCTGGCTGTTGAAATACCCGGAACATGCCCTTACCGGCCTGCTGCCTGCGGCGCTCGGCAAAGCCGGTGAAGCACAGGATAACGCCCGCGCTGCCTTGCGTATGCTTACCGAAAACGGTCATCAGCCATTACTGCAAGAAATCGCCCGACGTTATAACCAGCCGGAAGTAACCGATGCGGTGAACGCTCTGCTTGCGCTCGATCCCTTAGATAATCACCCGACAAAAATCCCCACTCTTCCGGC  >  NC_000913/2198553‑2198821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A