Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,327,508	A→G	G212G (GGT→GGC)	yfaP ←	DUF2135 domain‑containing protein YfaP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,327,508	0	A	G	100.0%	139.9 / NA	40	G212G (GGT→GGC)	yfaP	DUF2135 domain‑containing protein YfaP
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/16); total (24/16)

GTCAAAACTTTT‑CACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGG  >  NC_000913/2327373‑2327641

gTCAAAACTTTT‑CACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCGCCAt                                                                                                                                   >  1:770027/1‑140 (MQ=255)

agaaaaaCTTTTCCCCCGGCGTCAGCCCCCCACCATTCCGCATCGGTAAAAAAATTTTTTCTTTTTTCTCATTGCCCGACCCTTCATCGGTGATCAGCGTGAGTGGGGTGGTGTTTATTTCCTTTCCGCGGCGCccgcca                                                                                                                                    <  2:439477/137‑1 (MQ=255)

 tCAAAACTTTT‑CACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCGCCAta                                                                                                                                  >  1:1254881/1‑140 (MQ=255)

 tCAAAACTTTT‑CACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCGCCAta                                                                                                                                  >  1:522769/1‑140 (MQ=255)

        tttt‑CACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTAACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCGCCATAGTAGTTg                                                                                                                           >  2:277111/1‑140 (MQ=255)

           t‑CACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCGCCATAGTAGTTGata                                                                                                                        >  1:1240547/1‑140 (MQ=255)

                                            ggTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCggg                                                                                        >  2:1060935/1‑140 (MQ=255)

                                                   tAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCg                                                                                 >  2:591882/1‑140 (MQ=255)

                                                   tAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCATGAATTGGCGCGGGCATGGCg                                                                                 >  2:885127/1‑140 (MQ=255)

                                                      aTGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGaaa                                                                              >  2:1009691/1‑140 (MQ=255)

                                                       tGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCCTgtctctta                                                                             >  1:762708/1‑133 (MQ=255)

                                                          ttcttttttCTCATTGCCCGTTCTTCCATCGGTGATCGGCGTGGGTGGGGGGGTTTTTATTTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAAtct                                                                          <  2:1045440/134‑1 (MQ=255)

                                                          ttCCTTTTTCTCATTGACCGATCCTTCATGGGTGATCAGCGTCAGTGGGGGGGTGTTTATTTCCTTTTCGCTGCACCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAAtct                                                                          <  2:1105176/140‑1 (MQ=255)

                                                          tccttttttCTCTTTGCCCGTTCTTTCAGGGGTGAGCGGGGGGAGTGGGGGGGTGTTAATTTCCGTCTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAAtct                                                                          <  2:671838/134‑1 (MQ=255)

                                                           tCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAAtctc                                                                         >  2:562779/1‑140 (MQ=255)

                                                                  tctcATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCg                                                                  >  1:1045440/1‑140 (MQ=255)

                                                                       ttGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTAccc                                                             >  1:215763/1‑140 (MQ=255)

                                                                              aTCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTg                                                      >  1:1004164/1‑140 (MQ=255)

                                                                              aTCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTg                                                      >  1:1090779/1‑140 (MQ=255)

                                                                               tctttCATGGGTGACCAGCGTCGGTGGGGGGGTGTTTATTTCCTTTCCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGa                                                     <  1:799568/137‑1 (MQ=255)

                                                                               tCCTTCATGGGTGATCAGGGTCAGTGGGGGGGTGTTTATTTCCTTTTCGCGGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGa                                                     <  2:380581/140‑1 (MQ=255)

                                                                               tCCTTCATGGGTGATCAGCGTGGGTGGGGGGGTGTTTATTTCCTTTCCGCGCCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGa                                                     <  2:932471/140‑1 (MQ=255)

                                                                               tCCTTCATGGGTGATCAGCGTCGGTGGGGGGGTGTTTATTCCTGTTCCGCGGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGa                                                     <  2:700820/140‑1 (MQ=255)

                                                                               cctttCAGCGGGGATCAGCGTGGGTGGGGGGGTGTTTATTTCCGTTCCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGa                                                     <  2:1004164/137‑1 (MQ=255)

                                                                                 cTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACa                                                   >  1:914726/1‑140 (MQ=255)

                                                                                  ttcaggggTGATCAGCGTGGGTGGGGGGGTTTTTATTTCCTTTTCGCTGCCCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACAt                                                  <  2:914726/134‑1 (MQ=255)

                                                                                      ggggTGATCAGCGTCGGTGGGGGGGTGTTTATTTCCGTTCCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCat                                              <  2:215763/138‑1 (MQ=255)

                                                                                         gTGATCGGCGTGGGTGGGGGGGTGTTTATTTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATc                                           <  2:225129/140‑1 (MQ=255)

                                                                                         gTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATc                                           >  1:1155682/1‑140 (MQ=255)

                                                                                          tGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATcc                                          >  2:913475/1‑140 (MQ=255)

                                                                                                 cGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGcac                                   >  1:1149065/1‑140 (MQ=255)

                                                                                                            cGGTGGTTAATTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTc                        >  2:1003840/1‑140 (MQ=255)

                                                                                                              gtggtTAATTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAg                      <  1:1009691/140‑1 (MQ=255)

                                                                                                              gtggtTAATTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAg                      >  2:558782/1‑140 (MQ=255)

                                                                                                                ttttAATTCTGTTCCGCGGCACCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCa                    <  2:961339/138‑1 (MQ=255)

                                                                                                                ggtTAATTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCa                    <  1:591882/140‑1 (MQ=255)

                                                                                                                     aTTCCGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGgtg               >  1:621336/1‑140 (MQ=255)

                                                                                                                        ccttttCGCCGCCCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTa            <  1:246836/137‑1 (MQ=255)

                                                                                                                         cGTTTCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTAc           >  1:841842/1‑140 (MQ=255)

                                                                                                                           tttCGCTGCGCCCGCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACACCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCg         >  1:261804/1‑140 (MQ=255)

                                                                                                                                  gcgcCCGCCATAGTAGTTGATATACACCTGATAGCGCCCATGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGGACGGTGTTACCGTACCAgg  <  1:885127/140‑1 (MQ=255)

GTCAAAACTTTT‑CACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGG  >  NC_000913/2327373‑2327641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A