Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 260 73.5 2514320 99.3% 2496719 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,460,937 T→C intergenic (‑3/‑369) yfcZ ← / → fadL DUF406 domain‑containing protein YfcZ/long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,460,9370TC100.0% 214.9 / NA 60intergenic (‑3/‑369)yfcZ/fadLDUF406 domain‑containing protein YfcZ/long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/32);  total (28/32)

GCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCGGATAAGTGACCGAAATCACACTTAAAAATGATC  >  NC_000913/2460801‑2461068
                                                                                                                                        |                                                                                                                                   
gccAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGa                                                                                                                                  <  2:937694/140‑1 (MQ=255)
 ccAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGAt                                                                                                                                 <  1:1077591/140‑1 (MQ=255)
          gCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGt                                                                                                                        >  1:726988/1‑140 (MQ=255)
             ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTAtg                                                                                                                     <  2:432154/140‑1 (MQ=255)
                 ttCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCa                                                                                                                 <  1:631136/140‑1 (MQ=255)
                    tGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCAccc                                                                                                              >  2:408400/1‑140 (MQ=255)
                         ggTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGt                                                                                                         <  2:928835/140‑1 (MQ=255)
                         ggTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGt                                                                                                         <  1:560677/140‑1 (MQ=255)
                          gTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGt                                                                                                         >  2:433213/1‑139 (MQ=255)
                          gTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGt                                                                                                         <  1:433213/139‑1 (MQ=255)
                          gTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTg                                                                                                        >  2:268171/1‑140 (MQ=255)
                          gTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTg                                                                                                        >  1:119421/1‑140 (MQ=255)
                             tGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCa                                                                                                     >  2:42114/1‑140 (MQ=255)
                                  acacGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATaa                                                                                                <  2:960979/140‑1 (MQ=255)
                                   cacGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATaaa                                                                                               <  1:259569/140‑1 (MQ=255)
                                    acGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAAc                                                                                              <  2:752732/140‑1 (MQ=255)
                                     cgcgGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACg                                                                                             >  1:1246578/1‑140 (MQ=255)
                                       cgGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGtt                                                                                           >  1:789508/1‑140 (MQ=255)
                                          cTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTtgtg                                                                                        <  1:268171/140‑1 (MQ=255)
                                          cTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTtgtg                                                                                        <  2:726988/140‑1 (MQ=255)
                                           tGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTgg                                                                                       <  1:408400/140‑1 (MQ=255)
                                           tGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTgg                                                                                       <  1:836761/140‑1 (MQ=255)
                                              aaGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGata                                                                                    >  1:586523/1‑140 (MQ=255)
                                                  cgcgGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATAtttt                                                                                >  1:1056083/1‑140 (MQ=255)
                                                    cgGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTAc                                                                              >  1:323105/1‑140 (MQ=255)
                                                     ggTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACg                                                                             <  1:910854/140‑1 (MQ=255)
                                                        gCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCtt                                                                          <  2:119421/140‑1 (MQ=255)
                                                        gCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCtt                                                                          <  1:885618/140‑1 (MQ=255)
                                                              ggAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGaa                                                                    <  2:789508/140‑1 (MQ=255)
                                                                  ttGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGtg                                                                <  2:269026/140‑1 (MQ=255)
                                                                   tGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGtgc                                                               <  2:279125/140‑1 (MQ=255)
                                                                        aTAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTc                                                          <  2:323105/140‑1 (MQ=255)
                                                                               tGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTc                                                          <  1:66634/133‑1 (MQ=255)
                                                                               tGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTc                                                          >  2:66634/1‑133 (MQ=255)
                                                                               tGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAgttgt                                                   >  2:445575/1‑140 (MQ=255)
                                                                               tGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAgttgt                                                   >  1:480546/1‑140 (MQ=255)
                                                                                 ccAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTa                                                 >  1:102248/1‑140 (MQ=255)
                                                                                          aTACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCaa                                        <  1:900389/140‑1 (MQ=255)
                                                                                            aCAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCaaaa                                      <  1:42114/140‑1 (MQ=255)
                                                                                                cagcaAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCgg                                  >  2:222733/1‑140 (MQ=255)
                                                                                                cagcaAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCgg                                  >  2:7846/1‑140 (MQ=255)
                                                                                                cagcaAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCgg                                  >  1:403788/1‑140 (MQ=255)
                                                                                                cagcaAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCgg                                  >  1:715738/1‑140 (MQ=255)
                                                                                                 agcaAACCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCGGa                                 >  2:538996/1‑140 (MQ=255)
                                                                                                    aaaCCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCGGATaa                              <  1:7846/140‑1 (MQ=255)
                                                                                                    aaaCCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCGGATaa                              <  2:1056083/140‑1 (MQ=255)
                                                                                                     aaCCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCGGATAAg                             >  1:834716/1‑140 (MQ=255)
                                                                                                     aaCCGGGGTTTCATCAGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCGGATAAg                             >  2:952977/1‑140 (MQ=255)
                                                                                                                catcaGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCGGATAAGTGACCGAAATc                  <  1:222733/140‑1 (MQ=255)
                                                                                                                catcaGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCGGATAAGTGACCGAAATc                  >  1:1117051/1‑140 (MQ=255)
                                                                                                                  tcaGCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGCGCTGCTCCAGTTGTTAATTCTGCAAAATCGGATAAGTGACCGAAATcac                <  2:1246578/140‑1 (MQ=255)
                                                                                                                     gCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCGGATAAGTGACCGAAAt                   <  1:855060/134‑1 (MQ=255)
                                                                                                                     gCACTACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCGGATAAGTGACCGAAAt                   >  2:855060/1‑134 (MQ=255)
                                                                                                                         tACATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCGGATAAGTGACCGAAATCACACTTaaa         >  2:1041205/1‑140 (MQ=255)
                                                                                                                           cATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCGGATAAGTGACCGAAATCACACTTaaaaa       <  2:403788/140‑1 (MQ=255)
                                                                                                                           cATTTACTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCGGATAAGTGACCGAAATCACACTTaaaaa       <  1:445575/140‑1 (MQ=255)
                                                                                                                                aCTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCGGATAAGTGACCGAAATCACACTTAAAAATGATc  >  1:1031312/1‑140 (MQ=255)
                                                                                                                                aCTCATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCGGATAAGTGACCGAAATCACACTTAAAAATGATc  >  2:1246520/1‑140 (MQ=255)
                                                                                                                                   cATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCGGATAAGTGACCGAAATca                 <  1:185265/122‑1 (MQ=255)
                                                                                                                                   cATCGCTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCGGATAAGTGACCGAAATca                 >  2:185265/1‑122 (MQ=255)
                                                                                                                                        |                                                                                                                                   
GCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAAGGTGCCAGATAAACGTTGTGGATATTTTACGCTTCCGGAAAGTGCTGCTCCAGTTGTTAATTCTGCAAAATCGGATAAGTGACCGAAATCACACTTAAAAATGATC  >  NC_000913/2460801‑2461068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A