Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,806,989	T→C	V327A (GTT→GCT)	proW →	glycine betaine ABC transporter membrane subunit ProW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,806,989	0	T	C	100.0%	231.7 / NA	65	V327A (GTT→GCT)	proW	glycine betaine ABC transporter membrane subunit ProW
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (37/28); total (37/28)

CGCCTCGATGATTGCCGTCGGCGGGTTGGGTCAGATGGTACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGA  >  NC_000913/2806852‑2807125

cGCCTCGATGATTGCCGTCGGCGGGTTGGGTCAGATGGTACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTg                                                                                                                                        >  1:374369/1‑140 (MQ=255)

    tCGATGATTGCCGTCGGCGGGTTGGGTCAGATGGTACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGgcg                                                                                                                                    <  2:942564/140‑1 (MQ=255)

             gCCGTCGGCGGGTTGGGTCAGATGGTACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACg                                                                                                                           >  2:639866/1‑140 (MQ=255)

             gCCGTCGGCGGGTTGGGTCAGATGGTACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACg                                                                                                                           >  1:890551/1‑140 (MQ=255)

                gTCGGCGGGTTGGGTCAGATGGTACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAg                                                                                                                        <  2:1132726/140‑1 (MQ=255)

                        gTTGGGTCAGATGGTACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCa                                                                                                                <  2:374369/140‑1 (MQ=255)

                          tGGGTCAGATGGTACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAAc                                                                                                              <  2:569002/140‑1 (MQ=255)

                             gTCAGATGGTACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGt                                                                                                           <  2:435859/140‑1 (MQ=255)

                                aGATGGTACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGc                                                                                                        <  1:192919/140‑1 (MQ=255)

                                  aTGGTACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTg                                                                                                      >  1:1187365/1‑140 (MQ=255)

                                      tACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTac                                                                                                  >  1:385411/1‑140 (MQ=255)

                                           cgcgGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTa                                                                                                   <  1:987853/134‑1 (MQ=255)

                                           cgcgGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTa                                                                                                   >  2:987853/1‑134 (MQ=255)

                                           cgcgGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTAcaccac                                                                                             >  2:147179/1‑140 (MQ=255)

                                           cgcgGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTAcaccac                                                                                             >  2:411684/1‑140 (MQ=255)

                                           cgcgGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGACCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTAcaccac                                                                                             >  2:25408/1‑140 (MQ=255)

                                            gcgGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACt                                                                                            <  1:639866/140‑1 (MQ=255)

                                              ggTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTc                                                                                                          >  2:758494/1‑124 (MQ=255)

                                              ggTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTc                                                                                                          <  1:758494/124‑1 (MQ=255)

                                                   cGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTg                                                                                     <  1:766013/140‑1 (MQ=255)

                                                     gtcgtcTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGtt                                                                                   >  1:1136955/1‑140 (MQ=255)

                                                               tatGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCCCTGGCCCTGTTGGTCTGCTGa                                                                         <  2:890551/140‑1 (MQ=255)

                                                                 tGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGAcc                                                                       <  1:141080/140‑1 (MQ=255)

                                                                   gggCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCg                                                                     <  1:231118/140‑1 (MQ=255)

                                                                    ggCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGc                                                                    >  2:1253027/1‑140 (MQ=255)

                                                                     gCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGcc                                                                   <  1:857531/140‑1 (MQ=255)

                                                                       ttGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCca                                                                 >  2:495442/1‑140 (MQ=255)

                                                                        tGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGcc                                                                   >  1:557669/1‑137 (MQ=255)

                                                                        tGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGcc                                                                   <  2:557669/137‑1 (MQ=255)

                                                                        tGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCcat                                                                >  2:869147/1‑140 (MQ=255)

                                                                         gCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCcatt                                                               <  2:385411/140‑1 (MQ=255)

                                                                         gCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCcatt                                                               >  2:590904/1‑140 (MQ=255)

                                                                         gCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCcatt                                                               >  1:316518/1‑140 (MQ=255)

                                                                              cGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTa                                                          >  2:235013/1‑140 (MQ=255)

                                                                              cGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTa                                                          >  1:1107162/1‑140 (MQ=255)

                                                                                   gcggcgTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGACCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTaa                                                     <  1:25408/140‑1 (MQ=255)

                                                                                    cggcgTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAAc                                                    <  1:1253027/140‑1 (MQ=255)

                                                                                     ggcgTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAAct                                                   <  1:237368/140‑1 (MQ=255)

                                                                                       cgTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAActct                                                 >  1:664625/1‑140 (MQ=255)

                                                                                         tCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGc                                               >  2:1249594/1‑140 (MQ=255)

                                                                                              aTTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTg                                          <  1:1249594/140‑1 (MQ=255)

                                                                                                     tCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGt                                    <  1:245021/139‑1 (MQ=255)

                                                                                                     tCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGt                                    >  2:245021/1‑139 (MQ=255)

                                                                                                      ccTCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGa                                  >  2:410907/1‑140 (MQ=255)

                                                                                                        tCGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACg                                >  2:1198080/1‑140 (MQ=255)

                                                                                                         cGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACg                                >  2:1182505/1‑139 (MQ=255)

                                                                                                         cGCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACg                                <  1:1182505/139‑1 (MQ=255)

                                                                                                          gCCATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGcc                              <  1:301963/140‑1 (MQ=255)

                                                                                                           ccATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCg                             <  2:316518/140‑1 (MQ=255)

                                                                                                           ccATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCg                             <  1:151746/140‑1 (MQ=255)

                                                                                                           ccATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCg                             >  2:151746/1‑140 (MQ=255)

                                                                                                            cATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCgg                            >  1:543117/1‑140 (MQ=255)

                                                                                                            cATTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCgg                            <  2:1107162/140‑1 (MQ=255)

                                                                                                             aTTATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCggg                           <  1:1198080/140‑1 (MQ=255)

                                                                                                              ttATCCTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGc                          >  1:656463/1‑140 (MQ=255)

                                                                                                                   cTCGATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTAt                     >  2:471590/1‑140 (MQ=255)

                                                                                                                      gATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCAc                  >  1:267597/1‑140 (MQ=255)

                                                                                                                      gATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCAc                  >  2:973501/1‑140 (MQ=255)

                                                                                                                      gATCGTCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCAc                  >  2:32571/1‑140 (MQ=255)

                                                                                                                           tCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGc             >  1:213350/1‑140 (MQ=255)

                                                                                                                           tCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGc             >  2:399773/1‑140 (MQ=255)

                                                                                                                           tCTGACGCAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGc             >  1:856026/1‑140 (MQ=255)

                                                                                                                                  cAGGCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCaaaaaa      <  1:147179/140‑1 (MQ=255)

                                                                                                                                     gCCGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAgg   >  1:1067795/1‑140 (MQ=255)

                                                                                                                                      ccGCTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGa  >  2:891496/1‑140 (MQ=255)

CGCCTCGATGATTGCCGTCGGCGGGTTGGGTCAGATGGTACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGA  >  NC_000913/2806852‑2807125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A