Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,948,307	T→C	Y235C (TAC→TGC)	amiC ←	N‑acetylmuramoyl‑L‑alanine amidase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,948,307	0	T	C	100.0%	281.2 / NA	79	Y235C (TAC→TGC)	amiC	N‑acetylmuramoyl‑L‑alanine amidase C
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (39/40); total (39/40)

AAACACAGAGGAACCGCTCGGCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCACCGTGGCCAGGGTCAAGC  >  NC_000913/2948171‑2948438

aaaCACAGAGGAACCGCTCGGCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGcaca                                                                                                                                  >  1:523560/1‑140 (MQ=255)

 aaCACAGAGGAACCGCTCGGCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCacac                                                                                                                                 <  2:500240/140‑1 (MQ=255)

  acacAGAGGAACCGCTCGGCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACAcc                                                                                                                                <  1:930470/140‑1 (MQ=255)

       gagGAACCGCTCGGCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCat                                                                                                                           >  2:141326/1‑140 (MQ=255)

        agGAACCGCTCGGCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCata                                                                                                                          >  2:593920/1‑140 (MQ=255)

        agGAACCGCTCGGCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTAATGCACACCTTCata                                                                                                                          >  1:1047030/1‑140 (MQ=255)

         ggAACCGCTCGGCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCatat                                                                                                                         >  1:696753/1‑140 (MQ=255)

            aCCGCTCGGCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGc                                                                                                                      >  1:1118764/1‑140 (MQ=255)

             ccGCTCGGCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGcc                                                                                                                     >  1:319890/1‑140 (MQ=255)

             ccGCTCGGCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGcc                                                                                                                     >  1:388784/1‑140 (MQ=255)

             ccGCTCGGCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGcc                                                                                                                     <  2:388784/140‑1 (MQ=255)

               gCTCGGCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCct                                                                                                                   <  1:509325/140‑1 (MQ=255)

               gCTCGGCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCct                                                                                                                   <  2:523560/140‑1 (MQ=255)

                cTCGGCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCctc                                                                                                                  >  1:1140358/1‑140 (MQ=255)

                cTCGGCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCctc                                                                                                                  >  1:830169/1‑140 (MQ=255)

                 tCGGCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAGCAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCctct                                                                                                                 <  1:607665/140‑1 (MQ=255)

                 tCGGCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCctct                                                                                                                 <  2:1041184/140‑1 (MQ=255)

                   ggCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCttt                                                                                                               <  1:272437/140‑1 (MQ=255)

                   ggCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCttt                                                                                                               <  2:1140358/140‑1 (MQ=255)

                   ggCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCttt                                                                                                               <  2:830169/140‑1 (MQ=255)

                     cTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTtct                                                                                                             >  1:1000335/1‑140 (MQ=255)

                               gTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCagaga                                                                                                   >  2:296823/1‑140 (MQ=255)

                               gTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCagaga                                                                                                   >  2:447007/1‑140 (MQ=255)

                                     gCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCaa                                                                                             <  2:1151469/140‑1 (MQ=255)

                                      cGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCaaa                                                                                            >  1:459482/1‑140 (MQ=255)

                                         cGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACgg                                                                                         >  1:514305/1‑140 (MQ=255)

                                           gCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCg                                                                                       <  2:1199235/140‑1 (MQ=255)

                                             aTGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAg                                                                                     <  2:514305/140‑1 (MQ=255)

                                             aTGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAg                                                                                     <  1:1131317/140‑1 (MQ=255)

                                               ggATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCt                                                                                   <  2:1100166/140‑1 (MQ=255)

                                                      gaCAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCa                                                                            >  2:1099272/1‑140 (MQ=255)

                                                          aaCAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAg                                                                                     <  2:992671/127‑1 (MQ=255)

                                                          aaCAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAg                                                                                     >  1:992671/1‑127 (MQ=255)

                                                          aaCAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATAc                                                                        <  2:1118764/140‑1 (MQ=255)

                                                          aaCAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATAc                                                                        >  1:144446/1‑140 (MQ=255)

                                                          aaCAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATAc                                                                        <  1:249979/140‑1 (MQ=255)

                                                          aaCAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATAc                                                                        >  1:293100/1‑140 (MQ=255)

                                                          aaCAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATAc                                                                        >  1:359764/1‑140 (MQ=255)

                                                          aaCAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATAc                                                                        >  2:512203/1‑140 (MQ=255)

                                                          aaCAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATAc                                                                        >  1:642161/1‑140 (MQ=255)

                                                           aCAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATAcc                                                                       >  2:757126/1‑140 (MQ=255)

                                                               gTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAg                                                                                     <  2:977414/122‑1 (MQ=255)

                                                               gTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAg                                                                                     >  1:977414/1‑122 (MQ=255)

                                                               gTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACAt                                                                   >  2:540316/1‑140 (MQ=255)

                                                               gTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACAt                                                                   >  2:1098990/1‑140 (MQ=255)

                                                                        cTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTAATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTcgc                                                          <  2:1047030/140‑1 (MQ=255)

                                                                         tGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATc                                                                  <  1:1141975/131‑1 (MQ=255)

                                                                         tGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATc                                                                  >  2:1141975/1‑131 (MQ=255)

                                                                         tGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTcgcg                                                         >  1:197896/1‑140 (MQ=255)

                                                                         tGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTcgcg                                                         >  2:71621/1‑140 (MQ=255)

                                                                           tttCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTcgcgcg                                                       <  2:1254762/140‑1 (MQ=255)

                                                                             tCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGtt                                                     >  2:165625/1‑140 (MQ=255)

                                                                              cTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGttt                                                    <  2:319890/140‑1 (MQ=255)

                                                                                   cTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTAt                                               >  2:670896/1‑140 (MQ=255)

                                                                                        gCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTccc                                          <  2:773042/140‑1 (MQ=255)

                                                                                          tACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCca                                        <  2:1000335/140‑1 (MQ=255)

                                                                                                           gAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCg                       >  2:759430/1‑140 (MQ=255)

                                                                                                           gAATGAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCg                       <  1:670896/140‑1 (MQ=255)

                                                                                                               gAAGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCAc                   <  1:1088184/140‑1 (MQ=255)

                                                                                                                aaGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCAcc                  <  1:1099272/140‑1 (MQ=255)

                                                                                                                aaGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCAcc                  <  2:696753/140‑1 (MQ=255)

                                                                                                                aaGATGTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCAcc                  <  1:1029065/140‑1 (MQ=255)

                                                                                                                     gTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCACCGTGGc             >  2:861195/1‑140 (MQ=255)

                                                                                                                     gTCTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCACCGTGGc             >  1:750480/1‑140 (MQ=255)

                                                                                                                       cTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCACCGTGGCCa           <  2:684485/140‑1 (MQ=255)

                                                                                                                       cTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCACCGTGGCCa           <  1:757126/140‑1 (MQ=255)

                                                                                                                       cTTCATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCACCGTGGCCa           <  1:1152535/140‑1 (MQ=255)

                                                                                                                          cATTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCACCGTGGCCAggg        >  2:1051228/1‑140 (MQ=255)

                                                                                                                           aTTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCACCGTGGCCAGGGt       >  1:1215089/1‑140 (MQ=255)

                                                                                                                           aTTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCACCGTGGCCAGGGt       <  2:359764/140‑1 (MQ=255)

                                                                                                                           aTTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCACCGTGGCCAGGGt       <  2:293100/140‑1 (MQ=255)

                                                                                                                           aTTGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCACCGTGGCCAGGGt       <  1:593920/140‑1 (MQ=255)

                                                                                                                            ttGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCACCGTGGCCAGGGTc      <  2:508977/140‑1 (MQ=255)

                                                                                                                            ttGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCACCGTGGCCAGGGTc      <  2:1215089/140‑1 (MQ=255)

                                                                                                                            ttGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCACCGTGGCCAGGGTc      <  1:447007/140‑1 (MQ=255)

                                                                                                                             tGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCACCGTGGCCAGGGTCa     >  2:746032/1‑140 (MQ=255)

                                                                                                                             tGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCACCGTGGCCAGGGTCa     <  1:296823/140‑1 (MQ=255)

                                                                                                                             tGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCACCGTGGCCAGGGTCa     >  1:31294/1‑140 (MQ=255)

                                                                                                                             tGCGCGTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCACCGTGGCCAGGGTCa     >  1:1072140/1‑140 (MQ=255)

                                                                                                                                gcgTCATGCACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCACCGTGGCCAGGGTCAAGc  >  1:181581/1‑140 (MQ=255)

AAACACAGAGGAACCGCTCGGCTGACGACTGGTAAAGGCGTCGGCATGGATAGAGACAAACAGGTCAGCACGCTGTTTCTGGGCTTTTGCTACGCGCACTTGCAATGGAATGAAGATGTCTTCATTGCGCGTCATGTACACCTTCATATTGCCCTCTTTCTCGATCAGAGAGCGCAAACGGCGAGCTATTTGCAATACCACATCTTTTTCGCGCGTTTTGTATTTCCCCACCGCACCGGAGTCTTCGCCACCGTGGCCAGGGTCAAGC  >  NC_000913/2948171‑2948438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A