Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 260 73.5 2514320 99.3% 2496719 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,209,379 T→C G452G (GGT→GGC)  ttdT → L‑tartrate:succinate antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,209,3790TC100.0% 210.1 / NA 59G452G (GGT→GGC) ttdTL‑tartrate:succinate antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/32);  total (27/32)

ATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTC  >  NC_000913/3209242‑3209514
                                                                                                                                         |                                                                                                                                       
atgatgatTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTa                                                                                                                                       >  1:836645/1‑140 (MQ=255)
atgatgatTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTa                                                                                                                                       >  1:200771/1‑140 (MQ=255)
          ctgctgcGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAAcg                                                                                                                             >  2:1213652/1‑140 (MQ=255)
             ctgcGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCCAcggcg                                                                                                                          >  1:707778/1‑140 (MQ=255)
             ctgcGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAAcggcg                                                                                                                          <  1:1229394/140‑1 (MQ=255)
               gcgcTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGa                                                                                                                        <  1:430313/140‑1 (MQ=255)
                     gCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTg                                                                                                                  >  1:1232260/1‑140 (MQ=255)
                               aaaTCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTATTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTgggg                                                                                                        >  2:867669/1‑140 (MQ=255)
                                            ggTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGc                                                                                           <  2:200771/140‑1 (MQ=255)
                                                 tCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGata                                                                                      <  2:903488/140‑1 (MQ=255)
                                                     ccTGATGGTTGGTGCGGCAATTGGTCT‑GGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCt                                                                                 >  1:847715/1‑140 (MQ=255)
                                                      cTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCt                                                                                 <  2:556110/140‑1 (MQ=255)
                                                        gATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCg                                                                               <  1:930913/140‑1 (MQ=255)
                                                          tggttggtGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTa                                                                             <  2:609618/140‑1 (MQ=255)
                                                                 tGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTgg                                                                      <  1:53488/140‑1 (MQ=255)
                                                                       aaTTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTa                                                                <  1:787611/140‑1 (MQ=255)
                                                                          tGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCg                                                             <  1:198754/140‑1 (MQ=255)
                                                                             tCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCt                                                          <  1:97919/140‑1 (MQ=255)
                                                                               tGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTa                                                        <  2:1232260/140‑1 (MQ=255)
                                                                               ctgggAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTa                                                        <  2:847715/138‑1 (MQ=255)
                                                                                      cATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGa                                                 <  2:1000988/140‑1 (MQ=255)
                                                                                      cATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGa                                                 >  2:268574/1‑140 (MQ=255)
                                                                                      cATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGGGATTACCGGCTTACTGTGGa                                                 >  2:743612/1‑140 (MQ=255)
                                                                                       aTTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGAt                                                >  1:1043624/1‑140 (MQ=255)
                                                                                              cacCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCgtg                                         <  1:45509/140‑1 (MQ=255)
                                                                                                  aTACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGgtgt                                     <  2:1043624/140‑1 (MQ=255)
                                                                                                   tACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGGTTTTTGGACTGATATTCCTCGTATTGCTGGGGATTACCGGCTTACTGTGGATGCCCGTGGTGtt                                    >  1:1014418/1‑140 (MQ=255)
                                                                                                   tACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACtgtg                                                   >  2:295291/1‑125 (MQ=255)
                                                                                                   tACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACtgtg                                                   <  1:295291/125‑1 (MQ=255)
                                                                                                   tACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGa                                                 <  1:1077142/127‑1 (MQ=255)
                                                                                                   tACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGa                                                 >  2:1077142/1‑127 (MQ=255)
                                                                                                   tACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGAt                                                >  2:316829/1‑128 (MQ=255)
                                                                                                   tACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGAt                                                <  1:316829/128‑1 (MQ=255)
                                                                                                   tACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGtt                                    >  1:755540/1‑140 (MQ=255)
                                                                                                   tACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGtt                                    >  2:484467/1‑140 (MQ=255)
                                                                                                   tACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGtt                                    >  2:1243893/1‑140 (MQ=255)
                                                                                                     cGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCgtggt                                       <  2:956801/135‑1 (MQ=255)
                                                                                                     cGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCgtggt                                       >  1:956801/1‑135 (MQ=255)
                                                                                                     cGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGgtgt                                     >  1:554885/1‑137 (MQ=255)
                                                                                                     cGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGgtgt                                     <  2:554885/137‑1 (MQ=255)
                                                                                                     cGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGc                                  >  1:192450/1‑140 (MQ=255)
                                                                                                     cGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGc                                  >  1:735119/1‑140 (MQ=255)
                                                                                                     cGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGc                                  <  2:707778/140‑1 (MQ=255)
                                                                                                       ccACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCtt                                <  2:735119/140‑1 (MQ=255)
                                                                                                       ccACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCtt                                <  1:1243893/140‑1 (MQ=255)
                                                                                                       ccACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCtt                                <  1:484467/140‑1 (MQ=255)
                                                                                                        cACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCgtggt                                       <  2:1013381/132‑1 (MQ=255)
                                                                                                        cACCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCgtggt                                       >  1:1013381/1‑132 (MQ=255)
                                                                                                         aCCGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTa                              <  1:1078664/140‑1 (MQ=255)
                                                                                                          ccGGCCCCAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGTCCGTGGTGTTGCTTTaa                             <  2:836645/140‑1 (MQ=255)
                                                                                                              ccccAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGa                         >  2:764982/1‑140 (MQ=255)
                                                                                                                 cAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGTGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGa                      <  1:586687/140‑1 (MQ=255)
                                                                                                                 cAGTCCGATTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGa                      <  2:502869/140‑1 (MQ=255)
                                                                                                                        aTTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGcc               >  1:1047185/1‑140 (MQ=255)
                                                                                                                        aTTTACTACGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGcc               >  1:836622/1‑140 (MQ=255)
                                                                                                                           tactacGGCAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGa            <  1:1213652/140‑1 (MQ=255)
                                                                                                                                   cAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGctc    >  2:691067/1‑140 (MQ=255)
                                                                                                                                   cAGTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGctc    >  2:109160/1‑140 (MQ=255)
                                                                                                                                     gTGGCTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCtctc  <  1:867669/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
ATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTC  >  NC_000913/3209242‑3209514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A