Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	129,575	A→G	R562R (CGT→CGC)	yacH ←	DUF3300 domain‑containing protein YacH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	129,575	0	A	G	100.0%	174.3 / NA	49	R562R (CGT→CGC)	yacH	DUF3300 domain‑containing protein YacH
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (25/24); total (25/24)

CGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGATGCGGACTGAATACGCTCACGCGCTGCGTCTCTTCGCTGCTGGTTCTGC  >  NC_000913/129444‑129707

cGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTggcg                                                                                                                              <  2:838387/140‑1 (MQ=255)

cGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTggcg                                                                                                                              >  1:678936/1‑140 (MQ=255)

cGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTggcg                                                                                                                              <  1:547698/140‑1 (MQ=255)

  ttCGCCTACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCCGTTCTCCTGGACTTTCTCCCGCAAAACATGACGGTGTGCAGGTGATGCCGATTGAACGCGCTggcggg                                                                                                                            <  1:462526/140‑1 (MQ=255)

  ttCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTggcggg                                                                                                                            <  2:36444/140‑1 (MQ=255)

          ctgctgACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCa                                                                                                                    <  2:794570/140‑1 (MQ=255)

           tgctgACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTATTGTTGTTTAGACGTTGTGGGCGGCTCTCCTGAATTTTCTCCCGAAAAACCTTACGTTGTTCAGGTGATGCCGATTGAACGCGCTggcgggcgcttacgg                                                                                                                   >  2:462526/1‑133 (MQ=255)

           tgctgACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCAc                                                                                                                   >  2:813887/1‑140 (MQ=255)

           tgctgACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCAc                                                                                                                   >  1:457640/1‑140 (MQ=255)

            gctgACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACg                                                                                                                  >  1:32542/1‑140 (MQ=255)

             ctgACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGt                                                                                                                 >  2:856815/1‑140 (MQ=255)

             ctgACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGt                                                                                                                 >  1:5620/1‑140 (MQ=255)

              tgACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGtt                                                                                                                <  1:813887/140‑1 (MQ=255)

               gACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGt                                                                                                                 <  2:59218/138‑1 (MQ=255)

               gACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGt                                                                                                                 >  1:59218/1‑138 (MQ=255)

                aCCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGc                                                                                                              <  1:856815/140‑1 (MQ=255)

                  cGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTg                                                                                                            <  2:355918/140‑1 (MQ=255)

                     tGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATc                                                                                                                     >  2:368738/1‑128 (MQ=255)

                     tGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATc                                                                                                                     <  1:368738/128‑1 (MQ=255)

                     tGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTg                                                                                                         >  1:195282/1‑140 (MQ=255)

                                      gACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCgctgct                                                                                        >  2:483769/1‑140 (MQ=255)

                                      gACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCgctgct                                                                                        >  2:719124/1‑140 (MQ=255)

                                      gACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCgctgct                                                                                        >  2:713495/1‑140 (MQ=255)

                                      gACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCgctgc                                                                                         <  1:272309/139‑1 (MQ=255)

                                      gACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCgctgc                                                                                         >  2:272309/1‑139 (MQ=255)

                                        cAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGt                                                                                      >  2:37998/1‑140 (MQ=255)

                                             tGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACttt                                                                                 >  1:596783/1‑140 (MQ=255)

                                                ttACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTc                                                                              <  2:674759/140‑1 (MQ=255)

                                                ttACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTc                                                                              <  1:37998/140‑1 (MQ=255)

                                                  aCTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCt                                                                            >  1:598271/1‑140 (MQ=255)

                                                  aCTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCt                                                                            >  1:265128/1‑140 (MQ=255)

                                                     gTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACttt                                                                                 <  1:449089/132‑1 (MQ=255)

                                                     gTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACttt                                                                                 >  2:449089/1‑132 (MQ=255)

                                                     gTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTaa                                                                         >  2:763375/1‑140 (MQ=255)

                                                     gTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTaa                                                                         >  1:237235/1‑140 (MQ=255)

                                                     gTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTaa                                                                         >  1:570751/1‑140 (MQ=255)

                                                         tttAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGTGCGCTGCTGTACTTTTTCCTTAAacac                                                                     <  2:237235/140‑1 (MQ=255)

                                                          ttAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACAcc                                                                    <  1:483769/140‑1 (MQ=255)

                                                           tAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCt                                                                   >  1:309009/1‑140 (MQ=255)

                                                             gACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTgg                                                                 <  2:678936/140‑1 (MQ=255)

                                                              aCGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGgc                                                                <  2:32542/140‑1 (MQ=255)

                                                               cGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGgcg                                                               <  2:596783/140‑1 (MQ=255)

                                                               cGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGgcg                                                               <  2:457640/140‑1 (MQ=255)

                                                                gTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGgcgc                                                              <  1:248760/140‑1 (MQ=255)

                                                                    tgGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCgctgct                                                          >  1:562047/1‑140 (MQ=255)

                                                                                 tGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGATGCGGa                                             <  2:562047/140‑1 (MQ=255)

                                                                                                        aCGTTGTTCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGATGCGGACTGAATACGCTCACGCGCTGCGt                      <  1:719124/140‑1 (MQ=255)

                                                                                                          gttgttCAGGTGATGCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGATGCGGACTGAATACGCTCACGCGCTGCGtct                    <  2:570751/140‑1 (MQ=255)

                                                                                                                        gCCGATTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGATGCGGACTGAATACGCTCACGCGCTGCGTCTCTTCGCTGCTGGtt      >  1:227409/1‑140 (MQ=255)

                                                                                                                            aTTGAACGCGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGATGCGGACTGAATACGCTCACGCGCTGCGTCTCTTCGCTGCTGGTTCTGc  >  2:652011/1‑140 (MQ=255)

CGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGATGCGGACTGAATACGCTCACGCGCTGCGTCTCTTCGCTGCTGGTTCTGC  >  NC_000913/129444‑129707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A