Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,425,603	T→C	V76A (GTT→GCT)	ynaK →	Rac prophage; ParB‑like nuclease domain‑containing protein YnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,425,603	0	T	C	100.0%	136.6 / NA	42	V76A (GTT→GCT)	ynaK	Rac prophage; ParB‑like nuclease domain‑containing protein YnaK
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/25); total (17/25)

GTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGTTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATGGATGCCAGTTTGCACGTCGGAACGAAGGAGTTTGAT  >  NC_000913/1425476‑1425728

gTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCt                                                                                                                   >  1:306555/1‑140 (MQ=255)

      aGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGa                                                                                                             <  2:306555/140‑1 (MQ=255)

        tATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATg                                                                                                           >  1:63818/1‑140 (MQ=255)

                 aTTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAagc                                                                                                  <  2:218122/140‑1 (MQ=255)

                  ttCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGGTTCTGTTCCGGTCATTGCTCTGTCTGGGCCGACGGGTGAGCAGAagca                                                                                                 >  2:860696/1‑140 (MQ=255)

                  ttCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAagca                                                                                                 >  2:280747/1‑140 (MQ=255)

                  ttCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAagca                                                                                                 >  1:845691/1‑140 (MQ=255)

                       cTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGAt                                                                                            >  2:725226/1‑140 (MQ=255)

                        tGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATa                                                                                           <  1:152057/140‑1 (MQ=255)

                        tGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATa                                                                                           <  1:725226/140‑1 (MQ=255)

                            cTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGa                                                                                       <  1:280747/140‑1 (MQ=255)

                             tAATCCGGTGTTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGAt                                                                                      <  2:151362/140‑1 (MQ=255)

                             tAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGAt                                                                                      <  2:420642/140‑1 (MQ=255)

                                   ggTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTAt                                                                                <  2:664746/140‑1 (MQ=255)

                                   ggTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTAt                                                                                <  2:420314/140‑1 (MQ=255)

                                    gTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGTTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGt                                                                                  <  2:773244/137‑1 (MQ=255)

                                    gTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGt                                                                                  >  1:773244/1‑137 (MQ=255)

                                    gTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATc                                                                               >  1:564085/1‑140 (MQ=255)

                                    gTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATc                                                                               >  1:452499/1‑140 (MQ=255)

                                     tGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATca                                                                              <  1:656855/140‑2 (MQ=255)

                                     tGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCg                                                                              <  2:452499/140‑1 (MQ=255)

                                      gCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGc                                                                             <  2:564085/140‑1 (MQ=255)

                                        tGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTc                                                                           <  2:733923/140‑1 (MQ=255)

                                           ttGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCg                                                                        <  2:101363/140‑1 (MQ=255)

                                           ttGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCg                                                                        <  2:162282/140‑1 (MQ=255)

                                               cGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAGATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAAt                                                                    >  1:555854/1‑140 (MQ=255)

                                                  aaaGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCa                                                                 <  2:454546/140‑1 (MQ=255)

                                                      ggCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTaa                                                             <  1:400907/140‑1 (MQ=255)

                                                        cGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATc                                                           <  2:704107/140‑1 (MQ=255)

                                                                   cGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACAt                                                <  2:63818/140‑1 (MQ=255)

                                                                     ggTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTg                                              >  1:161154/1‑140 (MQ=255)

                                                                            gTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAAt                                       <  1:410555/140‑1 (MQ=255)

                                                                                tgtTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATGGAt                                   >  1:459164/1‑140 (MQ=255)

                                                                                tgtTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATGGAt                                   >  1:99806/1‑140 (MQ=255)

                                                                                     tGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGGCATTGCTCTGTCTGGCCTGACGGCAGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATGGATGCCAg                              >  1:300575/1‑140 (MQ=255)

                                                                                       gcggcTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATGGATGCCAGtt                            >  2:488442/1‑140 (MQ=255)

                                                                                          gcTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATGGATGCCAGTTTGc                         >  1:479685/1‑140 (MQ=255)

                                                                                          gcTGAAAAGCTCAAAATGGATTCTGTTTCGGTCATTGCTCTGTCTGGCCTTACGGATGAGCAGAAGCAGCGATAACGATCAATATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAAAGGATGCCCGTTTGc                         >  2:773531/1‑140 (MQ=255)

                                                                                           cTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATGGATGCCAGTTTGCa                        <  1:722024/140‑1 (MQ=255)

                                                                                              aaaTGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATGGATGCCAGTTTGCACGt                     >  2:429148/1‑140 (MQ=255)

                                                                                              aaaTGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATGGATGCCAGTTTGCACGt                     <  1:291699/140‑1 (MQ=255)

                                                                                              aaaTGCTCAAAATGGATTCTGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATGGATGCCAGTTTGCACGt                     <  2:845691/140‑1 (MQ=255)

                                                                                                                 tGTTCCGGTCATTGCTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATGGATGCCAGTTTGCACGTCGGAACGAAGGAGTTTGat  <  1:32061/140‑1 (MQ=255)

GTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGTTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATGGATGCCAGTTTGCACGTCGGAACGAAGGAGTTTGAT  >  NC_000913/1425476‑1425728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A