Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,879,225	G→A	L11F (CTT→TTT)	yeaQ ←	PF04226 family protein YeaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,879,225	0	G	A	100.0%	169.9 / NA	49	L11F (CTT→TTT)	yeaQ	PF04226 family protein YeaQ
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/22); total (27/22)

ATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTTTT  >  NC_000913/1879088‑1879360

aTCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAAcc                                                                                                                                       <  2:482495/140‑1 (MQ=255)

              aGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCAt                                                                                                                         <  1:219932/140‑1 (MQ=255)

               gCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATg                                                                                                                        >  2:676983/1‑140 (MQ=255)

               gCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATg                                                                                                                        >  2:181325/1‑140 (MQ=255)

                   aaCAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAg                                                                                                                    <  2:870464/140‑1 (MQ=255)

                         gTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGa                                                                                                                   >  1:163379/1‑135 (MQ=255)

                         gTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGa                                                                                                                   <  2:163379/135‑1 (MQ=255)

                          tGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATg                                                                                                                        >  2:609870/1‑129 (MQ=255)

                          tGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATg                                                                                                                        <  1:609870/129‑1 (MQ=255)

                          tGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGccc                                                                                                             <  2:575554/140‑1 (MQ=255)

                          tGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGccc                                                                                                             >  1:575554/1‑140 (MQ=255)

                                    tccGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGaa                                                                                                                  >  2:83656/1‑125 (MQ=255)

                                    tccGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGaa                                                                                                                  <  1:83656/125‑1 (MQ=255)

                                           aCTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTAt                                                                                            <  1:140111/140‑1 (MQ=255)

                                                   aCCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTg                                                                                    <  2:799490/140‑1 (MQ=255)

                                                     cGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCa                                                                                  <  2:470819/140‑1 (MQ=255)

                                                     cGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCa                                                                                  >  2:847679/1‑140 (MQ=255)

                                                       aCTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTAt                                                                                            >  2:457007/1‑128 (MQ=255)

                                                       aCTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTAt                                                                                            <  1:457007/128‑1 (MQ=255)

                                                                  cagGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGt                                                                     <  2:581680/140‑1 (MQ=255)

                                                                     gATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGc                                                                  >  1:477813/1‑140 (MQ=255)

                                                                     gATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGc                                                                  <  2:797986/140‑1 (MQ=255)

                                                                       tAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAg                                                                              <  1:401475/126‑1 (MQ=255)

                                                                       tAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAg                                                                              >  2:401475/1‑126 (MQ=255)

                                                                       tAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTg                                                                <  1:181325/140‑1 (MQ=255)

                                                                            aTAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATaaa                                                           <  2:700642/140‑1 (MQ=255)

                                                                            aTAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATaaa                                                           >  2:569201/1‑140 (MQ=255)

                                                                               aaGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGc                                                                  >  1:810537/1‑130 (MQ=255)

                                                                               aaGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGc                                                                  <  2:810537/130‑1 (MQ=255)

                                                                                    tCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCg                                                                   >  2:589184/1‑124 (MQ=255)

                                                                                    tCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCg                                                                   <  1:589184/124‑1 (MQ=255)

                                                                                       cccTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGcac                                                <  2:353802/139‑1 (MQ=255)

                                                                                            cATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACAt                                           >  1:796524/1‑140 (MQ=255)

                                                                                            cATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACAt                                           >  2:656273/1‑140 (MQ=255)

                                                                                                   aCCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAgg                                    >  2:206992/1‑140 (MQ=255)

                                                                                                   aCCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAgg                                    >  1:572964/1‑140 (MQ=255)

                                                                                                        gCATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAg                               >  1:214885/1‑140 (MQ=255)

                                                                                                         cATGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGa                              >  1:52158/1‑140 (MQ=255)

                                                                                                          aTGATCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGAt                             <  1:676983/140‑1 (MQ=255)

                                                                                                             aTCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTtg                          >  1:631830/1‑140 (MQ=255)

                                                                                                              tCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTtgt                         >  2:772/1‑140 (MQ=255)

                                                                                                              tCCACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTtgt                         >  2:315514/1‑140 (MQ=255)

                                                                                                               ccACTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTtgtg                        <  1:847679/140‑1 (MQ=255)

                                                                                                                  cTTCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACg                     >  1:791230/1‑140 (MQ=255)

                                                                                                                   ttCGCCAGAATACCGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGa                    >  2:472589/1‑140 (MQ=255)

                                                                                                                               ccGGCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAgg        <  1:123685/140‑1 (MQ=255)

                                                                                                                                 ggCAATAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGtt      >  2:606504/1‑140 (MQ=255)

                                                                                                                                     aTAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGtttttt  >  2:349031/1‑140 (MQ=255)

                                                                                                                                     aTAAACCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGtttttt  >  2:322177/1‑140 (MQ=255)

ATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTTTT  >  NC_000913/1879088‑1879360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A