Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F28 I1 R1
|
227 |
52.0 |
1764248 |
99.3% |
1751898 |
138.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,880,220 |
C→G |
Q180H (CAG→CAC) |
leuE ← |
leucine exporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,880,220 | 0 | C | G | 100.0%
| 90.3
/ NA
| 26 | Q180H (CAG→CAC) | leuE | leucine exporter |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (8/18); total (8/18) |
AGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATT > NC_000913/1880086‑1880327
|
aGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGt > 1:392368/1‑140 (MQ=255)
gggAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAGCCCACGAACATCAAACCAATCAGTGAGTTGCCGACTTTACCCAGTTTTTTTTTGGTACGGATGTAGTGCGTGACa > 2:822290/1‑140 (MQ=255)
gggAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACa > 2:94107/1‑140 (MQ=255)
gggAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACa > 2:584742/1‑140 (MQ=255)
gCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGc > 1:201735/1‑140 (MQ=255)
cccGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCa < 2:437276/140‑1 (MQ=255)
ccGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCAc < 1:284341/140‑1 (MQ=255)
ccGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCAc < 1:20631/140‑1 (MQ=255)
ccGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCAc < 1:254854/140‑1 (MQ=255)
ccGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCAc < 2:582962/140‑1 (MQ=255)
ccGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCAc < 1:576559/140‑1 (MQ=255)
ccGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCAc < 2:56285/140‑1 (MQ=255)
ccGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCAc < 1:678050/140‑1 (MQ=255)
ccGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCAc < 1:96632/140‑1 (MQ=255)
ccGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCAc < 2:386924/140‑1 (MQ=255)
cgcgGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCAc > 1:184597/1‑131 (MQ=255)
cgcgGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCAc < 2:184597/131‑1 (MQ=255)
aaaGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCACCAGATATAATCAGGaggc < 1:584742/140‑4 (MQ=255)
tCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAAt > 1:571379/1‑140 (MQ=255)
gCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGaaa > 1:36472/1‑140 (MQ=255)
cAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAAc < 1:42772/140‑1 (MQ=255)
tCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTcgccg < 2:41957/126‑1 (MQ=255)
tCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTcgccg > 1:41957/1‑126 (MQ=255)
aGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTAtgt < 2:36472/140‑1 (MQ=255)
ttGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTgg < 2:392368/140‑1 (MQ=255)
aCTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCAtt < 1:94107/140‑1 (MQ=255)
aCTTTAGCCAGTTTCTTTTTGGTACGTATGTAGTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCAtt < 2:571379/140‑1 (MQ=255)
|
AGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATT > NC_000913/1880086‑1880327
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
